Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of the transcriptional co-activator SAGA complex, including the histone acetyltransferase module.
Journal, issue, pages	Mol Cell, Vol. 85, Issue 23, Page 4333-44346.e4, Year 2025
Publish date	Dec 4, 2025
Authors	Rayees U H Mattoo / Dong-Hua Chen / David A Bushnell / Sagi Tamir / Roger D Kornberg /
PubMed Abstract	The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four ...The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four modules: transcription-associated protein 1 (Tra1), core, deubiquitination (DUB), and histone acetyltransferase (HAT). Although the structures of the Tra1, core, and DUB modules have been determined, the overall architecture of the HAT module remained elusive due to its inherent flexibility. To address this, we conducted cryo-electron microscopy (cryo-EM) analyses on SAGA purified from the thermophilic fungus Chaetomium thermophilum, yielding structures of Tra1 and core modules at 2.6 Å and three of the four HAT subunits at 3.7 Å. The structure of the HAT module was informative about the aspects of histone acetylation and the interface of HAT-core modules, contradicting earlier AlphaFold predictions. Our structure-guided genetic and biochemical analyses confirmed the roles of Ada1 and Spt7 in anchoring the HAT module within the SAGA complex.
External links	Mol Cell / PubMed:41260211 / PubMed Central
Methods	EM (single particle)
Resolution	2.6 - 4.4 Å
Structure data	73233 9ynu EMDB-73233, PDB-9ynu: Tra1 and core modules including core tip of ctSAGA complex Method: EM (single particle) / Resolution: 3.2 Å EMDB-73234: Tra1 and Core Module of ctSAGA Method: EM (single particle) / Resolution: 2.7 Å 73235 9ynv EMDB-73235, PDB-9ynv: Histone Acetyl Transferase (HAT) module of ctSAGA Method: EM (single particle) / Resolution: 3.7 Å 73236 9ynw EMDB-73236, PDB-9ynw: Tra1, Core and minimal HAT modules of ctSAGA, composite. Method: EM (single particle) / Resolution: 3.5 Å 73237 9yzy EMDB-73237: Focused map of HAT module Bromodomain region PDB-9yzy: ctSAGA Focused on HAT Bromodomain region Method: EM (single particle) / Resolution: 4.4 Å EMDB-73238: Focused map of Tra1 module of ctSAGA Method: EM (single particle) / Resolution: 3.7 Å EMDB-73239: Focused map of Tra1 module of ctSAGA Method: EM (single particle) / Resolution: 3.4 Å EMDB-73240: Focused map of Tra1 module of ctSAGA complex Method: EM (single particle) / Resolution: 3.0 Å EMDB-73241: Focused map of core module of ctSAGA complex Method: EM (single particle) / Resolution: 2.9 Å EMDB-73242: Focused map of core module of ctSAGA complex Method: EM (single particle) / Resolution: 2.8 Å EMDB-73243: Focused map of core module of ctSAGA complex Method: EM (single particle) / Resolution: 2.9 Å 73261 9yol EMDB-73261, PDB-9yol: Tra1 module of ctSAGA Method: EM (single particle) / Resolution: 2.6 Å 73269 9yoq EMDB-73269, PDB-9yoq: Core module of ctSAGA Method: EM (single particle) / Resolution: 2.6 Å
Source	thermochaetoides thermophila (fungus)
Keywords	TRANSCRIPTION / ctSAGA complex / Histone Acetyl Transferase (HAT) Module / Tra1 module and Core module