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- EMDB-73261: Tra1 module of ctSAGA -

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Basic information

Entry
Database: EMDB / ID: EMD-73261
TitleTra1 module of ctSAGA
Map data
Sample
  • Complex: Tra1 module of ctSAGA complex
    • Protein or peptide: SCA7 domain-containing protein
    • Protein or peptide: Spt20-like SEP domain-containing protein
    • Protein or peptide: Chains: C
    • Protein or peptide: Putative transcriptional coactivator HFI1 protein
    • Protein or peptide: Transcription initiation factor TFIID subunit 12
    • Protein or peptide: Non-specific serine/threonine protein kinase
KeywordsctSAGA complex / Histone Acetyl Transferase (HAT) Module / Tra1 module and Core module / TRANSCRIPTION
Function / homology
Function and homology information


RITS complex assembly / regulatory ncRNA-mediated heterochromatin formation / SAGA complex / transcription factor TFIID complex / NuA4 histone acetyltransferase complex / RNA polymerase II preinitiation complex assembly / TBP-class protein binding / transcription coregulator activity / transcription by RNA polymerase II / transcription coactivator activity ...RITS complex assembly / regulatory ncRNA-mediated heterochromatin formation / SAGA complex / transcription factor TFIID complex / NuA4 histone acetyltransferase complex / RNA polymerase II preinitiation complex assembly / TBP-class protein binding / transcription coregulator activity / transcription by RNA polymerase II / transcription coactivator activity / protein heterodimerization activity / DNA repair / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / DNA binding / nucleus
Similarity search - Function
Transcription factor Spt20 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Spt20-like, SEP domain / Spt20, SEP domain / Transcriptional regulator of RNA polII, SAGA, subunit / SAGA-associated factor 73 / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region ...Transcription factor Spt20 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Spt20-like, SEP domain / Spt20, SEP domain / Transcriptional regulator of RNA polII, SAGA, subunit / SAGA-associated factor 73 / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Transcription initiation factor IID, subunit 13 / Transcription initiation factor IID, 18kD subunit / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / Transcription initiation factor TFIID subunit 12 domain / : / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Histone-fold / Armadillo-like helical / Armadillo-type fold / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Spt20-like SEP domain-containing protein / Transcription initiation factor TFIID subunit 12 domain-containing protein / Uncharacterized protein / SCA7 domain-containing protein / Non-specific serine/threonine protein kinase / Putative transcriptional coactivator HFI1 protein
Similarity search - Component
Biological speciesThermochaetoides thermophila (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsMattoo RUH / Chen DH / Bushnell DA / Tamir S / Kornberg RD
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM049985 United States
CitationJournal: Mol Cell / Year: 2025
Title: Structure of the transcriptional co-activator SAGA complex, including the histone acetyltransferase module.
Authors: Rayees U H Mattoo / Dong-Hua Chen / David A Bushnell / Sagi Tamir / Roger D Kornberg /
Abstract: The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four ...The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four modules: transcription-associated protein 1 (Tra1), core, deubiquitination (DUB), and histone acetyltransferase (HAT). Although the structures of the Tra1, core, and DUB modules have been determined, the overall architecture of the HAT module remained elusive due to its inherent flexibility. To address this, we conducted cryo-electron microscopy (cryo-EM) analyses on SAGA purified from the thermophilic fungus Chaetomium thermophilum, yielding structures of Tra1 and core modules at 2.6 Å and three of the four HAT subunits at 3.7 Å. The structure of the HAT module was informative about the aspects of histone acetylation and the interface of HAT-core modules, contradicting earlier AlphaFold predictions. Our structure-guided genetic and biochemical analyses confirmed the roles of Ada1 and Spt7 in anchoring the HAT module within the SAGA complex.
History
DepositionOct 13, 2025-
Header (metadata) releaseDec 3, 2025-
Map releaseDec 3, 2025-
UpdateDec 3, 2025-
Current statusDec 3, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_73261.png

Downloads & links

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Map

FileDownload / File: emd_73261.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 360 pix.
= 374.76 Å		1.04 Å/pix.
x 360 pix.
= 374.76 Å		1.04 Å/pix.
x 360 pix.
= 374.76 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.041 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.8
Minimum - Maximum-5.619441 - 8.598271
Average (Standard dev.)0.0067339637 (±0.15551855)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 374.76 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_73261_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_73261_half_map_2.map
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Sample components

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Entire : Tra1 module of ctSAGA complex

EntireName: Tra1 module of ctSAGA complex
Components
  • Complex: Tra1 module of ctSAGA complex
    • Protein or peptide: SCA7 domain-containing protein
    • Protein or peptide: Spt20-like SEP domain-containing protein
    • Protein or peptide: Chains: C
    • Protein or peptide: Putative transcriptional coactivator HFI1 protein
    • Protein or peptide: Transcription initiation factor TFIID subunit 12
    • Protein or peptide: Non-specific serine/threonine protein kinase

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Supramolecule #1: Tra1 module of ctSAGA complex

SupramoleculeName: Tra1 module of ctSAGA complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Thermochaetoides thermophila (fungus)

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Macromolecule #1: SCA7 domain-containing protein

MacromoleculeName: SCA7 domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 137.082672 KDa
SequenceString: MDSRKDEEAT IKVAPAKGTV LASKPHIWGK LKLKKPPVKQ IKPGNWKEAT LVEDKKKDKD GGAINAASPG PDVNQLDDRA RDTFSNGRP LEDVLELLQC KLCRKGISRT AARDHIAKCL QIKKEKAQRK KEAREARERL KEAAREEEAR RLEEEGAAKG D DDSDDDDG ...String:
MDSRKDEEAT IKVAPAKGTV LASKPHIWGK LKLKKPPVKQ IKPGNWKEAT LVEDKKKDKD GGAINAASPG PDVNQLDDRA RDTFSNGRP LEDVLELLQC KLCRKGISRT AARDHIAKCL QIKKEKAQRK KEAREARERL KEAAREEEAR RLEEEGAAKG D DDSDDDDG KEGAGKAGKK TKKTDVAGKK RKAEGELDKG KAKKKKDEPK PKVAKPKGPV DVERQCGVPL PNGQLCARSL TC KSHSMSA KRAVPGRSLP YDMLLAAYQK KNQAKQQKAA LDANAPLEDE DDLNAGAVDS DEETAAVMSA LANWNPQPVV PQP VFAPLK RQYQLARLHE QLQAATNGGR TNIFKVVGFG AQRLSDGHLG LYDAEDAPGE PDPVGLGSLS RRSSSIVAGE DDTT QQAQI AAVDHESQKE DAPLAEVCKK PCWKPKDRPG SVLDQEYVPM SGRSLSKVTK LKATGRAAAN RQPKQSRRAA SAIPE SSKP LADAPVNTIV SKSSPALPTA TPLYAFANLP TTDLRLQPAE LAIQEMRKRL IAASSSSQSS ISSTGVRAPR GRKRKE DRS MPGIHGSQPM ENIHRATDGV RQTPENMAPT PKESPSDSTV EMRVLELGQL LAQEDSLAKS SPVLPNTPVV SLLGTSQ NG ATNGLKQGVQ HIQRQMATKP TSVSRAISHS PMSSRPLFSA PSPPMMSHSL SPLTRAATPK ALSSSPLVMS MLLPQQPP S HPFVSLPSGS TLSQAMQQSP IMTSMSKSGL TPAATRQPSP GVMPHSPPPR QGPGRPPKPR PADPVSPVNP TRSPNLPNA SYLTTLGDLS TGMQPPQLPH MPQQQVPRVK LNQMRNQALA STFSPRPSYL PSQRLDPIRE QAIKRAAERA AGLRMAQAQR QPQMQVHMN VQAQRTPQLQ ARTSPPTASP AETQGQMLSA RAQAEDQGQN RLRMGQPQAN TQLMRIDGPG MAAGMRQPIG N SQMDTMQP QRQVMNTPVV PAPQSSSPPS AERSSSQPQP VRDQIDSASV QAEAAQDETM NLDNDEMDDI LREVKAQAER EA ERQMAAL SRTRPQAQQQ AQNLRSTQNQ SQMHALSPVA SESSSPSLVM SASLPGLNEV NVRATVAAAA KKVAGSISPV ANI SSAATA LSSPLDCRPY TTTSIPRPRL ATTAPVPLMG ATGPPAASGT TQNIGSFHVQ QHRQRRQQQQ LLQQHAQQHQ QRLQ QAAVH NNQQPFRFTY PAESMAAAVA IAGAHNLRPV VPLPPQAYLL SASQGGGRSG SRGSNIRNGS QARTQEQG

UniProtKB: SCA7 domain-containing protein

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Macromolecule #2: Spt20-like SEP domain-containing protein

MacromoleculeName: Spt20-like SEP domain-containing protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 128.449367 KDa
SequenceString: MAPTVTAAAP PVNPPSATKV KRPIPPGIQT NGVAPSRSSP SPSMAAKRAP SAVRQPSNPP SAGGTTPGAT RPTIRIRRDP GPGPPLGRG QRSAGLRSAS IATDPVTSPT FDAPPYVITP EYILKKFAGH PPSLIVHLYQ NHFRFDQQEG MFQYKSPMRI F IEHLRNRT ...String:
MAPTVTAAAP PVNPPSATKV KRPIPPGIQT NGVAPSRSSP SPSMAAKRAP SAVRQPSNPP SAGGTTPGAT RPTIRIRRDP GPGPPLGRG QRSAGLRSAS IATDPVTSPT FDAPPYVITP EYILKKFAGH PPSLIVHLYQ NHFRFDQQEG MFQYKSPMRI F IEHLRNRT VPHEIMEYLI QGGVPFYEGC LIVQVFDHRT VNQTKEAAKP SSESSKTVPF SIHNHNPYIT PSPYVPYPKE DT PADGSQT PASEKPSEDK DKENMPAPPV PADGQKAKGE PAKPTVYTVV LMPTAQALHT DLLLKTVTPR GASDSGGPPP TPH TAGPPP TPTAAGMPPP AKKQKRDHME LDPKNIYEVE AKILLATYPK LDLEPTKNAE ETIAKLEKLA HPEHSHKPPE PKVR KRTVA EVAHDEALAA EQERYMLTLD ERLSSKLAGA QAGANGADGD GQAPSASWEP RFERFKLIEN IKQEHAEKKE QERIK QAEQ ERKLLQQKQE AAILQARQQQ AEMERKRQEQ IVLQQRQEAQ RRLQQAQAAP VAQAQAQAQA QAQAQAQAQA QAQARA QAR AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAAQAQ AQ AQAQAQAQAQ AQAQAQAQAQ AQQAATAATM GQTPHAHPMQ PSPLPAGMTA GMPVSMAQQA QARFSQVSQP QQMSSPIV Q QGTPQAASSP MPPMAAVAMQ HSNSNMGQAG SPPRPPSVVQ NHPMGVPMAP SMSARGSQQS HAAGTPRVPS ATPNIPHAT PITRPQVVPT PRMTQASPPA GVMTPTPQQM GQAMLMNNQG IAQMPNVNIN SLAAAQQMAA QQRLIQQYQQ QLQQSGMVPG NVQANMLQQ QLAARQQMMI QQQLLAQQQQ RAGQMMNPNI QAQLAAQYQQ SLNNMQQQQL AQMNPMQAAH MRQLLAAQQA Q RQGMMGVN VNVNGMTPQQ LAVMQQQIQL QQQAQLIQQQ QQQQQQLQQQ QQQQQQQQQQ QQQQQQQQQQ QQAAQQQPQP GM PGHPPQA AVAPTAPQVP IAQLQPPQNQ LAALMMNPAV QNQIRNHSNA LYSKNLPALA ERFGGNAQNI PPEALAEFRQ KCL NQAKVQ VLSMLQQQQA GGRLAAGLPP QVQAAQVQAM QQQAQALAVA QAQVQAAQQA AAAQAAQQQG GMGQGM

UniProtKB: Spt20-like SEP domain-containing protein

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Macromolecule #3: Chains: C

MacromoleculeName: Chains: C / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 48.541484 KDa
SequenceString: MALSDNNPEE VDGMFLSNLR SDLELEEIWR MLQSGVTSPE DSQPTVSLDA SSSGDKTMSS ESQVALQDDA DDELLVSILA GKVPLSELL ANDNELLQSD GLSSAVCSAD SDQEEDDNND AGYGEEPDPR DVIANWFELC QMMYVSGETG EPSLETTGII E DIVRQQVI ...String:
MALSDNNPEE VDGMFLSNLR SDLELEEIWR MLQSGVTSPE DSQPTVSLDA SSSGDKTMSS ESQVALQDDA DDELLVSILA GKVPLSELL ANDNELLQSD GLSSAVCSAD SDQEEDDNND AGYGEEPDPR DVIANWFELC QMMYVSGETG EPSLETTGII E DIVRQQVI EIIRHDAPKV SRLRTFLAWK DVRKNVKDSD DKGADADIAP GDDPVGAVVP GATVDDTARK TKKSRVGLPW EP ASFYSVE VPEREDEEDE EEEEMNYITL QRLRKADERT KAMTKEEYVT WSEFRQASFT YRKGKRFREW AGFGLVTDSK PSD DIIDIL GFLTFEMVQT LTEEALKIKE QEDLHRERTG ENQSNANNPN GSANSSLSKK RKLTGGGGLF DPPNEGRTPV EPRH IQEAF RRLQQRPKKA RAMLNGTKLQ QRTQLKLF

UniProtKB: Uncharacterized protein

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Macromolecule #4: Putative transcriptional coactivator HFI1 protein

MacromoleculeName: Putative transcriptional coactivator HFI1 protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 52.133355 KDa
SequenceString: MVDINPAALS RPSIGLSTPS FPGKSGSINV SVPSSASASA SASSAHSKPQ KTSLVPPRID LEPIYTALKA AIGAEQWPVY KETLTNFLI GRLNQAELSE RIDPILASQD GQKEHLHNQL IAAIYANVTR EMPDPGLAPW VSANDKPTTA QTKPVTGDAN E RRLKGEVM ...String:
MVDINPAALS RPSIGLSTPS FPGKSGSINV SVPSSASASA SASSAHSKPQ KTSLVPPRID LEPIYTALKA AIGAEQWPVY KETLTNFLI GRLNQAELSE RIDPILASQD GQKEHLHNQL IAAIYANVTR EMPDPGLAPW VSANDKPTTA QTKPVTGDAN E RRLKGEVM QLPARDRRRI KELAHNDFDP YDALANVLME HARAKPPKIA EPPASAGGLK MNFDLEIRKR YAQPLAVESG EF PDTSNIE SRMLPFCYEA GLSSGHAPDA AQFMSIATET FIKEVMSAIF SRTRSNGPGD SGSAGFGLGS GWIQTHRYRK QLA KEEEAF QRGEISRDKM GLLPVEAKAA SERGPLGMAD VRLALEMGDC GLAGFPVIVK SVIYGWREGE LENWEDYTYV DPSR PDGIA IPSRGDGDVE MGGVGVNGVV KGEPEPQQQP LANGVHDVDA MDIDGVSVAG GAANEIWWEG AEPETSDLLN AVLDS CLAV N

UniProtKB: Putative transcriptional coactivator HFI1 protein

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Macromolecule #5: Transcription initiation factor TFIID subunit 12

MacromoleculeName: Transcription initiation factor TFIID subunit 12 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 83.091188 KDa
SequenceString: MNQTPAQGQG QGQPGQGMAN RPPNAPSAAN VAAPGGAPRQ QGTPMYRPEM MRNIPLLSDE EKTKYERGLQ QLWLMHDNTA PGSAENIEA KKKIAEFGRV LLTKLQQRRQ QQQLQQQRQQ QQQQQQQQQQ QQQQQQQVQQ QMQQQAQQPQ TQPQQGQPQQ Q LQQAQVQQ ...String:
MNQTPAQGQG QGQPGQGMAN RPPNAPSAAN VAAPGGAPRQ QGTPMYRPEM MRNIPLLSDE EKTKYERGLQ QLWLMHDNTA PGSAENIEA KKKIAEFGRV LLTKLQQRRQ QQQLQQQRQQ QQQQQQQQQQ QQQQQQQVQQ QMQQQAQQPQ TQPQQGQPQQ Q LQQAQVQQ QVQQQQQIPQ QQQQAPQQQQ QQQQPQQLGQ PGQQVQMMPQ NQQPQVAQNV QPVAPTTVPQ QQPAPATQTA PN QMGAPNS AAAQLRSLPA HIVSHVKDVT FLAPNNVVDK AGWIDQLKLQ YAQKLFVMDS AGRQIKQIAA SLADQSLPPE ERK KLEEKK QTLEKQYNDA ISFANTVRKQ YMQKPAQNGA AVQNAANVAR PQSAQGASAM TQPGAGVGNG TPVAAPVNPM QSSA AAINT AIETAKKQQM AAGRMAGAVN AMAAQVQSPA TPVQAQVSPA TQVHATQQSV PQQPQQIQQA QPTQQPQAQV KIEPG TAPH PAPLNTTMAG PTGVPSAAAP AQNAARMQTP TSATPTSANP NIRPLTHAAA MNLASQQRPG MPAVPTAASG AAPGTP MGI MGAAQQQQQQ QPGHSHAHPP QPTQAPPPHS TNPKLPIPKV LHDKAIQMPQ PVPNIGGAGS GRPTYTSGVM NQPALPK TP AFQLEGEGER VLNKKKLDEL VRQVCGGTAE GQDGNMLTPE VEESVLNMAD AFVDNVLHQA CRNAKERGSK VLEIRDIQ L VLERVYNIRI PGYASEELRT VRKVQPNSNW IKKMSAVQAA KVVPGKGDI

UniProtKB: Transcription initiation factor TFIID subunit 12 domain-containing protein

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Macromolecule #6: Non-specific serine/threonine protein kinase

MacromoleculeName: Non-specific serine/threonine protein kinase / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 444.670094 KDa
SequenceString: MATSLIEDVV KKLSVAETGT GNSLSERSLL VLPLMQVSCY TELKVKAEAA CALRDSLDHY TAGPAYPAFL KKVMPVFIHI LRQQPVFQT TGEQGHMQKL RNCILEILHR LPTSQTPPEH FEPYAHEIVD LLLELVRTDN EDNVILCVKI LSDIMRHQHK I MSGKVQTF ...String:
MATSLIEDVV KKLSVAETGT GNSLSERSLL VLPLMQVSCY TELKVKAEAA CALRDSLDHY TAGPAYPAFL KKVMPVFIHI LRQQPVFQT TGEQGHMQKL RNCILEILHR LPTSQTPPEH FEPYAHEIVD LLLELVRTDN EDNVILCVKI LSDIMRHQHK I MSGKVQTF LNLIQELFEQ LDKVVRDQLD NTSTTGPPGP PSTPGSTQSS FLPHQQSPRP GSPVTTGVTD FGADANQQAN RL LPRGMYS FKVLAECPII VVSIFQVHRS AVTPNVKAFV PLIKSALLCQ AKAQDQAHRE AAARGTIHIG VSPNIKNRAA FGD FITAQV KTMSFLAYLL RQYSSQLSDF LPHLPDIIVR LLRDCPRDKS SARKELFVAI RHIINFNFRK IFLPKIDELL DERT LVGDG LTVHETMRPL AYSMLADLIH HVRDQLSPEQ IRKTVEVYTR NLQDNFPGTS FQTMSAKLLL NMAECIARLP NKVDA RHYL IMILNAIADK FAAMNRQYPN AVKLSKQYAQ QAAKNVPETY LADKENPPDW DEIDIFNATP IKISNPRDRA ADPVVD NKF LFRNLMTGLK NTFYQLKSCN IPGAVDLTGA PAHWTDVAYG FTAEEVKVIA KLFREGAYVF RYYEIEKPAA ESPYSSP VE YMANFYMVSS SKEEKELLET FATVFHCIDP ATFHEVFQQE IPRLYEMIHE HTALLHIPQF FLASEATSPS FCGILLRF L MERIEDVGSA DIKKSSILLR LFKLAFMAVT LFANQNEQVL LPHVVDIVTK SIELSTKAEE PMNYFLLLRS LFRSIGGGK FEHLYKQILP LLEMLLDVLN NLLMAARKPA ERDLYVELCL TVPARLSNLL PHLSFLMRPL VVALRAGTEL VGQGLRTLEL CVDNLTADY LDPIMAPVID ELMTALFDHL KPHPYSHFHA HTTLRILGKL GGRNRKFMTE ALPVTFQQYV DDRASFDLRL I GSKRDRAF PAHLGIDLAI QKLTEIPKPV KGGVPQLSRQ YDPYYKRQAL NLIIAQTKLR VGFDSVPEDL PRLVRLQVED LV KKNRDVD ISLFEVSDKE RSIAKKNEEQ ALLKRLLKAL LYAESIPEFK QEVDALLVNL ARHFTLIEIG RSVIDMKKAY TPF DTKSGE GPLFIDNRVL SEAILESLAN ENLEVREAAK RLIKEMHNAA VTVFGSPMHV PRMTFWSSLA STFCHGCYEE EWFT KQGGA LGIQTLLTEI DLGDLWVAAK QIEFVRALMY VVKDMPQDLP EKTRRTAQVT LEQLLARITR NAKKQDCLPP PPPPA PPGS HPPQPPPRPP RLAQLVMMLN SELAHMNRHV RATARRSIEA IAAAAQAEVW ELLEPHKKHI LGPIYAKPLR ALPFAI QIG YIDAVTYYMS LKRDFVPFDE NLNRLLMESL ALADASDESL AGKTLEFRTH DFIVNLRVSC IKILSRAMSF EEFGSGS NN PTRPKVVSVF FKCLYSDSQP TIDAANDALK AVLQHTTKLP KDLLQSGLRP VLASLQDAKR LTVHSLDNLG RLLRLLTT Y FKVEIGTRLL EHVKQIADPN FLQQLSFTFF EQNNAMKVIA AVFNIFHLLP DAARQFKERV IDNVLDLEQK LRRTHHSPF RLPLYKYLNK YPSDIWAFTL QKLEDLRYGR FLAQVLRHPD SQVLRDYGAA NVEFIIKTCN TVVVSGKETK FIAIINTINM LDALCQFPT ANSQSWLDNK EHIEWLKTVG KELELQQKKN SLTPNLRLPA HQASEQLMNI LTKALDRNPN DLDALFSLVE G ITSDDFRE TPALLSHIYN KIIRSDSIDF WRITILRCLD IYSGKTASQR MKWYLLHNIV NPIIAMDVMR TWDDPNKASR LL DRTVIDS INTKIWKVNL PDPQEDLLQP RVDHTRMEVL QLSAMLVKYR HALLQDARKD FIKFAWTYIR LDDVINKHAA YVV IGYFIA HYETPAKIVS QIYFSLLKTN QNEGRALVTQ ALELMAPVMP KRCPFPPGDR NPVWALAPRR ILAEESQNVQ QMTA IFHYL VRHPDLFYDT RDKFAMLIIQ CLRKVASPPN PSNDNRKLAL NMMWLIWQWE QRRVESKLSE PLRAPSESPN SNKRK LDEQ QGLSPSATRQ AEFQIPSVGR HKMIRYLVEF IAQLNERYPL PSARPSDPPT TTSFLLPSPS NELCKKATAL LYNLLQ PQY WGDLVEEMDL FPNVTDLVLA GEKSATLLSA DPSDPEKCDD KLITSIINTL QVVRIVLHFK SDRWILDNMP QIQKILE KC LKSANPEIQD CLHMADRKYD GDRDVKPTIK RILDAVPEDI PMEDADGDIE PESQTSELIA WLSGIATESM AAGNYVSG I NILWSLGHRK PSIIDQHIPA IMRSLQSKLA RDHVSHYAAL GHQASGLRPQ ADPNAPTEMK AYDLEIQTNL ILKAIEVTA MRMEILGDNR RPFLSVLATI VEKSLHIPLC EKILEMVEGW VFRSEGTWPT LKEKTAVLHK MISFEHRTDP TMLHKFLNLV LRIYEDPKI TRTELTVRME HAFLIGTRAQ DVEMRNKFMS IFDKCLSKTA SARLAYVILG QNWDTLADSY WLAQASQLLL G AVDMNPTI QLHQDDFRTL PISQLVAPYA KDSREPSVMI DDKFEAFMAN HRRFLAELGD VRARDVLEPL MQLQHIDANF AH ELWVALF PMFWSAVPKE DRPDLERGLV TLLTKDYHSR QMDKRPNVVQ SLMAGAVKTW PHCKIPPHVL KFEAKTYDAW YTA LHQLEN AAIKPEIDSA AVRESNLDAL VDLYSTLGED DLFYGTWRRR CQFVETNAGL SYEQHGMWEK AQRMYESAQI KART GVIPF SEAEYMLWED HWVLCAQKLQ QWEILQDFAK HENFQDLLLE CAWRNTEYWQ NQENRDQLDT VIKGVMDAPT PRRYF FQAF MSLLKLHNKQ ETPQDFNRVV DEAIQLSIRK WHQLPKRLTA AHVPLLQNFQ QLVELHDASV ICQSLAQTNA SNLDVK SGE LKLLLGSWRD RLPNMWDDIV AWQDLVTWRQ HVFGLINATY LQLIPQQAQN AGGASFAYRG YHETAWIINR FAHVARK HA LPEVCISQLS RIYTLPNIEI QEAFLKLREQ AKCHYQNPDE LTSGLDVINN TNLNYFSAPQ KAEFYTLKGM FLEKLGQK E EADTAFGTAL YFDITAAKAW AEWGYFNDRK FKENPSDLPS AKQALTSYLQ AASSYKNHKS RKLIARILWL LSLDDANGT IAAGFDDFKG EIYVWWWIIF IPQLLVGLGY KEAPRVQAIL LKIAKTYPQA LYFLLRTNRE DMLTIKKNQE AKERARQRAA QAAIPNKPN GSPQLTKKDG AAGAADGRPP TANGETPKPP EIKIEGAAPN APTAATPQAA ANAPQVPGAA PAEQQTGGVQ K KPPWELTE EIMSVLKTAF PLLALSMETM VDQIQKHFKC PPDEDAYRLI VALLNDGLSY VSRMPASYAK DVKLPAATEN NI TRFAETI LPNHIRPTFE ADFVKVKPTM YEYIHNLRKW RDKFEEKLDR RASPAPLESF AHYSPHLSEF RYQKFDDVEI PGQ YLQHKD KNQDFIRIDR FLPNVDLVRT IGASHRRLRI RGHDGSVHAF AVQHPAARHC RREERVLQLF RQLNQTLANK KESR RRDLQ FTLPLMVPLA PHIRIVQEDT SYITLQSVYE DHCRRHGMNK DEPILFTMEK LRGLMDAKAL QKPEQAVTAK LEVYR AIQE KFVDHTIVLE YFQGAYPNFA DFWLFRRRFS YQLAALTFMT YTMHIDKRYP HKMNIARGSG NIWGSEAVSY MAPSRP LFQ NTEPVPFRLT PNLQTLMGPL ATEGIFAASV MAIARCLTEP EQHLEHALTL FVRDEMMFWF TNSHRTMSLT ENQLRET VQ ANCDMIVKKA LALAQTPQGN LPAHQTVTDL IAKATNPMNL ALCDALWMPY L

UniProtKB: Non-specific serine/threonine protein kinase

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Sugar embeddingMaterial: HEPES Buffer
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 68.9 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND / Type: NONE
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 1886619
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: PROJECTION MATCHING

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