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- EMDB-73236: Tra1, Core and minimal HAT modules of ctSAGA, composite. -

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Basic information

Entry
Database: EMDB / ID: EMD-73236
TitleTra1, Core and minimal HAT modules of ctSAGA, composite.
Map data
Sample
  • Complex: Tra1 module of ctSAGA complex
    • Protein or peptide: x 6 types
  • Protein or peptide: x 8 types
KeywordsctSAGA complex / Histone Acetyl Transferase (HAT) Module / Tra1 module and Core module / TRANSCRIPTION
Function / homology
Function and homology information


SAGA-type complex / RITS complex assembly / regulatory ncRNA-mediated heterochromatin formation / SLIK (SAGA-like) complex / histone H3 acetyltransferase activity / SAGA complex / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / NuA4 histone acetyltransferase complex / histone acetyltransferase complex ...SAGA-type complex / RITS complex assembly / regulatory ncRNA-mediated heterochromatin formation / SLIK (SAGA-like) complex / histone H3 acetyltransferase activity / SAGA complex / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / NuA4 histone acetyltransferase complex / histone acetyltransferase complex / histone acetyltransferase / RNA polymerase II preinitiation complex assembly / translation initiation factor activity / TBP-class protein binding / transcription coregulator activity / transcription initiation at RNA polymerase II promoter / promoter-specific chromatin binding / chromatin organization / transcription by RNA polymerase II / transcription coactivator activity / chromatin remodeling / protein heterodimerization activity / DNA repair / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / structural molecule activity / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleus
Similarity search - Function
Histone acetyltransferases subunit 3 / Histone acetyltransferases subunit 3 / ADA2 ZZ-type zing finger / Transcriptional activator Spt7 / Transcriptional adaptor 2 / ADA2-like, zinc finger, ZZ-type / : / Transcriptional adapter 2-alpha-like domain / Transcription factor Spt20 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 ...Histone acetyltransferases subunit 3 / Histone acetyltransferases subunit 3 / ADA2 ZZ-type zing finger / Transcriptional activator Spt7 / Transcriptional adaptor 2 / ADA2-like, zinc finger, ZZ-type / : / Transcriptional adapter 2-alpha-like domain / Transcription factor Spt20 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Spt20-like, SEP domain / Spt20, SEP domain / Transcriptional regulator of RNA polII, SAGA, subunit / SAGA-associated factor 73 / LisH / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Histone acetyltransferase GCN5 / Transcription initiation factor IID, subunit 13 / Transcription initiation factor IID, 18kD subunit / Bromodomain associated / Bromodomain associated domain / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / TATA box binding protein associated factor (TAF) / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain / Transcription initiation factor TFIID subunit 12 domain / TAF6, C-terminal HEAT repeat domain / Transcription initiation factor TFIID 23-30kDa subunit / Transcription initiation factor TFIID, 23-30kDa subunit / Myb-like domain profile. / Lissencephaly type-1-like homology motif / SWIRM domain / SWIRM domain / SWIRM domain profile. / LIS1 homology (LisH) motif profile. / LIS1 homology motif / : / FATC domain / PIK-related kinase, FAT / FAT domain / Myb-type HTH DNA-binding domain profile. / SANT domain profile. / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / SANT domain / Zinc finger ZZ-type signature. / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Myb domain / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Myb-like DNA-binding domain / Acetyltransferase (GNAT) family / Gcn5-related N-acetyltransferase (GNAT) domain profile. / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / GNAT domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / SANT/Myb domain / Acyl-CoA N-acyltransferase / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Homeobox-like domain superfamily / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Histone-fold / bromo domain / Bromodomain / Bromodomain (BrD) profile. / Bromodomain-like superfamily / Armadillo-like helical / WD domain, G-beta repeat / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / Winged helix-like DNA-binding domain superfamily / WD40-repeat-containing domain superfamily
Similarity search - Domain/homology
Spt20-like SEP domain-containing protein / Transcription initiation factor TFIID subunit 10 / SAGA complex subunit Spt7 / Transcription initiation factor TFIID subunit 12 domain-containing protein / Uncharacterized protein / SCA7 domain-containing protein / TBP-associated factor 6 / Transcriptional adapter 2 / Non-specific serine/threonine protein kinase / histone acetyltransferase ...Spt20-like SEP domain-containing protein / Transcription initiation factor TFIID subunit 10 / SAGA complex subunit Spt7 / Transcription initiation factor TFIID subunit 12 domain-containing protein / Uncharacterized protein / SCA7 domain-containing protein / TBP-associated factor 6 / Transcriptional adapter 2 / Non-specific serine/threonine protein kinase / histone acetyltransferase / Uncharacterized protein / Putative transcriptional coactivator HFI1 protein / Transcription initiation factor TFIID subunit-like protein
Similarity search - Component
Biological speciesThermochaetoides thermophila (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsMattoo RUH / Chen DH / Bushnell DA / Tamir S / Kornberg RD
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM049985 United States
CitationJournal: Mol Cell / Year: 2025
Title: Structure of the transcriptional co-activator SAGA complex, including the histone acetyltransferase module.
Authors: Rayees U H Mattoo / Dong-Hua Chen / David A Bushnell / Sagi Tamir / Roger D Kornberg /
Abstract: The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four ...The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four modules: transcription-associated protein 1 (Tra1), core, deubiquitination (DUB), and histone acetyltransferase (HAT). Although the structures of the Tra1, core, and DUB modules have been determined, the overall architecture of the HAT module remained elusive due to its inherent flexibility. To address this, we conducted cryo-electron microscopy (cryo-EM) analyses on SAGA purified from the thermophilic fungus Chaetomium thermophilum, yielding structures of Tra1 and core modules at 2.6 Å and three of the four HAT subunits at 3.7 Å. The structure of the HAT module was informative about the aspects of histone acetylation and the interface of HAT-core modules, contradicting earlier AlphaFold predictions. Our structure-guided genetic and biochemical analyses confirmed the roles of Ada1 and Spt7 in anchoring the HAT module within the SAGA complex.
History
DepositionOct 12, 2025-
Header (metadata) releaseDec 3, 2025-
Map releaseDec 3, 2025-
UpdateDec 3, 2025-
Current statusDec 3, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_73236.png

Downloads & links

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Map

FileDownload / File: emd_73236.map.gz / Format: CCP4 / Size: 446.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 501 pix.
= 521.541 Å		1.04 Å/pix.
x 483 pix.
= 502.803 Å		1.04 Å/pix.
x 484 pix.
= 503.844 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 1.041 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 7.0
Minimum - Maximum-0.91003317 - 71.845579999999998
Average (Standard dev.)0.42088902 (±1.0618199)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-2-2-19
Dimensions483484501
Spacing484483501
CellA: 503.844 Å / B: 502.803 Å / C: 521.541 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Tra1 module of ctSAGA complex

EntireName: Tra1 module of ctSAGA complex
Components
  • Complex: Tra1 module of ctSAGA complex
    • Protein or peptide: SCA7 domain-containing protein
    • Protein or peptide: Spt20-like SEP domain-containing protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Transcription initiation factor TFIID subunit 5
    • Protein or peptide: Transcription initiation factor TFIID subunit 6
    • Protein or peptide: Putative transcription initiation factor
  • Protein or peptide: Non-specific serine/threonine protein kinase
  • Protein or peptide: Transcription initiation factor TFIID subunit 10
  • Protein or peptide: Transcription initiation factor TFIID subunit 12
  • Protein or peptide: Putative transcriptional activator protein
  • Protein or peptide: Putative transcriptional coactivator HFI1 protein
  • Protein or peptide: Transcriptional adapter 2
  • Protein or peptide: histone acetyltransferase
  • Protein or peptide: Uncharacterized protein

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Supramolecule #1: Tra1 module of ctSAGA complex

SupramoleculeName: Tra1 module of ctSAGA complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #10, #2-#6
Source (natural)Organism: Thermochaetoides thermophila (fungus)

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Macromolecule #1: Non-specific serine/threonine protein kinase

MacromoleculeName: Non-specific serine/threonine protein kinase / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 444.670094 KDa
SequenceString: MATSLIEDVV KKLSVAETGT GNSLSERSLL VLPLMQVSCY TELKVKAEAA CALRDSLDHY TAGPAYPAFL KKVMPVFIHI LRQQPVFQT TGEQGHMQKL RNCILEILHR LPTSQTPPEH FEPYAHEIVD LLLELVRTDN EDNVILCVKI LSDIMRHQHK I MSGKVQTF ...String:
MATSLIEDVV KKLSVAETGT GNSLSERSLL VLPLMQVSCY TELKVKAEAA CALRDSLDHY TAGPAYPAFL KKVMPVFIHI LRQQPVFQT TGEQGHMQKL RNCILEILHR LPTSQTPPEH FEPYAHEIVD LLLELVRTDN EDNVILCVKI LSDIMRHQHK I MSGKVQTF LNLIQELFEQ LDKVVRDQLD NTSTTGPPGP PSTPGSTQSS FLPHQQSPRP GSPVTTGVTD FGADANQQAN RL LPRGMYS FKVLAECPII VVSIFQVHRS AVTPNVKAFV PLIKSALLCQ AKAQDQAHRE AAARGTIHIG VSPNIKNRAA FGD FITAQV KTMSFLAYLL RQYSSQLSDF LPHLPDIIVR LLRDCPRDKS SARKELFVAI RHIINFNFRK IFLPKIDELL DERT LVGDG LTVHETMRPL AYSMLADLIH HVRDQLSPEQ IRKTVEVYTR NLQDNFPGTS FQTMSAKLLL NMAECIARLP NKVDA RHYL IMILNAIADK FAAMNRQYPN AVKLSKQYAQ QAAKNVPETY LADKENPPDW DEIDIFNATP IKISNPRDRA ADPVVD NKF LFRNLMTGLK NTFYQLKSCN IPGAVDLTGA PAHWTDVAYG FTAEEVKVIA KLFREGAYVF RYYEIEKPAA ESPYSSP VE YMANFYMVSS SKEEKELLET FATVFHCIDP ATFHEVFQQE IPRLYEMIHE HTALLHIPQF FLASEATSPS FCGILLRF L MERIEDVGSA DIKKSSILLR LFKLAFMAVT LFANQNEQVL LPHVVDIVTK SIELSTKAEE PMNYFLLLRS LFRSIGGGK FEHLYKQILP LLEMLLDVLN NLLMAARKPA ERDLYVELCL TVPARLSNLL PHLSFLMRPL VVALRAGTEL VGQGLRTLEL CVDNLTADY LDPIMAPVID ELMTALFDHL KPHPYSHFHA HTTLRILGKL GGRNRKFMTE ALPVTFQQYV DDRASFDLRL I GSKRDRAF PAHLGIDLAI QKLTEIPKPV KGGVPQLSRQ YDPYYKRQAL NLIIAQTKLR VGFDSVPEDL PRLVRLQVED LV KKNRDVD ISLFEVSDKE RSIAKKNEEQ ALLKRLLKAL LYAESIPEFK QEVDALLVNL ARHFTLIEIG RSVIDMKKAY TPF DTKSGE GPLFIDNRVL SEAILESLAN ENLEVREAAK RLIKEMHNAA VTVFGSPMHV PRMTFWSSLA STFCHGCYEE EWFT KQGGA LGIQTLLTEI DLGDLWVAAK QIEFVRALMY VVKDMPQDLP EKTRRTAQVT LEQLLARITR NAKKQDCLPP PPPPA PPGS HPPQPPPRPP RLAQLVMMLN SELAHMNRHV RATARRSIEA IAAAAQAEVW ELLEPHKKHI LGPIYAKPLR ALPFAI QIG YIDAVTYYMS LKRDFVPFDE NLNRLLMESL ALADASDESL AGKTLEFRTH DFIVNLRVSC IKILSRAMSF EEFGSGS NN PTRPKVVSVF FKCLYSDSQP TIDAANDALK AVLQHTTKLP KDLLQSGLRP VLASLQDAKR LTVHSLDNLG RLLRLLTT Y FKVEIGTRLL EHVKQIADPN FLQQLSFTFF EQNNAMKVIA AVFNIFHLLP DAARQFKERV IDNVLDLEQK LRRTHHSPF RLPLYKYLNK YPSDIWAFTL QKLEDLRYGR FLAQVLRHPD SQVLRDYGAA NVEFIIKTCN TVVVSGKETK FIAIINTINM LDALCQFPT ANSQSWLDNK EHIEWLKTVG KELELQQKKN SLTPNLRLPA HQASEQLMNI LTKALDRNPN DLDALFSLVE G ITSDDFRE TPALLSHIYN KIIRSDSIDF WRITILRCLD IYSGKTASQR MKWYLLHNIV NPIIAMDVMR TWDDPNKASR LL DRTVIDS INTKIWKVNL PDPQEDLLQP RVDHTRMEVL QLSAMLVKYR HALLQDARKD FIKFAWTYIR LDDVINKHAA YVV IGYFIA HYETPAKIVS QIYFSLLKTN QNEGRALVTQ ALELMAPVMP KRCPFPPGDR NPVWALAPRR ILAEESQNVQ QMTA IFHYL VRHPDLFYDT RDKFAMLIIQ CLRKVASPPN PSNDNRKLAL NMMWLIWQWE QRRVESKLSE PLRAPSESPN SNKRK LDEQ QGLSPSATRQ AEFQIPSVGR HKMIRYLVEF IAQLNERYPL PSARPSDPPT TTSFLLPSPS NELCKKATAL LYNLLQ PQY WGDLVEEMDL FPNVTDLVLA GEKSATLLSA DPSDPEKCDD KLITSIINTL QVVRIVLHFK SDRWILDNMP QIQKILE KC LKSANPEIQD CLHMADRKYD GDRDVKPTIK RILDAVPEDI PMEDADGDIE PESQTSELIA WLSGIATESM AAGNYVSG I NILWSLGHRK PSIIDQHIPA IMRSLQSKLA RDHVSHYAAL GHQASGLRPQ ADPNAPTEMK AYDLEIQTNL ILKAIEVTA MRMEILGDNR RPFLSVLATI VEKSLHIPLC EKILEMVEGW VFRSEGTWPT LKEKTAVLHK MISFEHRTDP TMLHKFLNLV LRIYEDPKI TRTELTVRME HAFLIGTRAQ DVEMRNKFMS IFDKCLSKTA SARLAYVILG QNWDTLADSY WLAQASQLLL G AVDMNPTI QLHQDDFRTL PISQLVAPYA KDSREPSVMI DDKFEAFMAN HRRFLAELGD VRARDVLEPL MQLQHIDANF AH ELWVALF PMFWSAVPKE DRPDLERGLV TLLTKDYHSR QMDKRPNVVQ SLMAGAVKTW PHCKIPPHVL KFEAKTYDAW YTA LHQLEN AAIKPEIDSA AVRESNLDAL VDLYSTLGED DLFYGTWRRR CQFVETNAGL SYEQHGMWEK AQRMYESAQI KART GVIPF SEAEYMLWED HWVLCAQKLQ QWEILQDFAK HENFQDLLLE CAWRNTEYWQ NQENRDQLDT VIKGVMDAPT PRRYF FQAF MSLLKLHNKQ ETPQDFNRVV DEAIQLSIRK WHQLPKRLTA AHVPLLQNFQ QLVELHDASV ICQSLAQTNA SNLDVK SGE LKLLLGSWRD RLPNMWDDIV AWQDLVTWRQ HVFGLINATY LQLIPQQAQN AGGASFAYRG YHETAWIINR FAHVARK HA LPEVCISQLS RIYTLPNIEI QEAFLKLREQ AKCHYQNPDE LTSGLDVINN TNLNYFSAPQ KAEFYTLKGM FLEKLGQK E EADTAFGTAL YFDITAAKAW AEWGYFNDRK FKENPSDLPS AKQALTSYLQ AASSYKNHKS RKLIARILWL LSLDDANGT IAAGFDDFKG EIYVWWWIIF IPQLLVGLGY KEAPRVQAIL LKIAKTYPQA LYFLLRTNRE DMLTIKKNQE AKERARQRAA QAAIPNKPN GSPQLTKKDG AAGAADGRPP TANGETPKPP EIKIEGAAPN APTAATPQAA ANAPQVPGAA PAEQQTGGVQ K KPPWELTE EIMSVLKTAF PLLALSMETM VDQIQKHFKC PPDEDAYRLI VALLNDGLSY VSRMPASYAK DVKLPAATEN NI TRFAETI LPNHIRPTFE ADFVKVKPTM YEYIHNLRKW RDKFEEKLDR RASPAPLESF AHYSPHLSEF RYQKFDDVEI PGQ YLQHKD KNQDFIRIDR FLPNVDLVRT IGASHRRLRI RGHDGSVHAF AVQHPAARHC RREERVLQLF RQLNQTLANK KESR RRDLQ FTLPLMVPLA PHIRIVQEDT SYITLQSVYE DHCRRHGMNK DEPILFTMEK LRGLMDAKAL QKPEQAVTAK LEVYR AIQE KFVDHTIVLE YFQGAYPNFA DFWLFRRRFS YQLAALTFMT YTMHIDKRYP HKMNIARGSG NIWGSEAVSY MAPSRP LFQ NTEPVPFRLT PNLQTLMGPL ATEGIFAASV MAIARCLTEP EQHLEHALTL FVRDEMMFWF TNSHRTMSLT ENQLRET VQ ANCDMIVKKA LALAQTPQGN LPAHQTVTDL IAKATNPMNL ALCDALWMPY L

UniProtKB: Non-specific serine/threonine protein kinase

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Macromolecule #2: Spt20-like SEP domain-containing protein

MacromoleculeName: Spt20-like SEP domain-containing protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 128.449367 KDa
SequenceString: MAPTVTAAAP PVNPPSATKV KRPIPPGIQT NGVAPSRSSP SPSMAAKRAP SAVRQPSNPP SAGGTTPGAT RPTIRIRRDP GPGPPLGRG QRSAGLRSAS IATDPVTSPT FDAPPYVITP EYILKKFAGH PPSLIVHLYQ NHFRFDQQEG MFQYKSPMRI F IEHLRNRT ...String:
MAPTVTAAAP PVNPPSATKV KRPIPPGIQT NGVAPSRSSP SPSMAAKRAP SAVRQPSNPP SAGGTTPGAT RPTIRIRRDP GPGPPLGRG QRSAGLRSAS IATDPVTSPT FDAPPYVITP EYILKKFAGH PPSLIVHLYQ NHFRFDQQEG MFQYKSPMRI F IEHLRNRT VPHEIMEYLI QGGVPFYEGC LIVQVFDHRT VNQTKEAAKP SSESSKTVPF SIHNHNPYIT PSPYVPYPKE DT PADGSQT PASEKPSEDK DKENMPAPPV PADGQKAKGE PAKPTVYTVV LMPTAQALHT DLLLKTVTPR GASDSGGPPP TPH TAGPPP TPTAAGMPPP AKKQKRDHME LDPKNIYEVE AKILLATYPK LDLEPTKNAE ETIAKLEKLA HPEHSHKPPE PKVR KRTVA EVAHDEALAA EQERYMLTLD ERLSSKLAGA QAGANGADGD GQAPSASWEP RFERFKLIEN IKQEHAEKKE QERIK QAEQ ERKLLQQKQE AAILQARQQQ AEMERKRQEQ IVLQQRQEAQ RRLQQAQAAP VAQAQAQAQA QAQAQAQAQA QAQARA QAR AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAAQAQ AQ AQAQAQAQAQ AQAQAQAQAQ AQQAATAATM GQTPHAHPMQ PSPLPAGMTA GMPVSMAQQA QARFSQVSQP QQMSSPIV Q QGTPQAASSP MPPMAAVAMQ HSNSNMGQAG SPPRPPSVVQ NHPMGVPMAP SMSARGSQQS HAAGTPRVPS ATPNIPHAT PITRPQVVPT PRMTQASPPA GVMTPTPQQM GQAMLMNNQG IAQMPNVNIN SLAAAQQMAA QQRLIQQYQQ QLQQSGMVPG NVQANMLQQ QLAARQQMMI QQQLLAQQQQ RAGQMMNPNI QAQLAAQYQQ SLNNMQQQQL AQMNPMQAAH MRQLLAAQQA Q RQGMMGVN VNVNGMTPQQ LAVMQQQIQL QQQAQLIQQQ QQQQQQLQQQ QQQQQQQQQQ QQQQQQQQQQ QQAAQQQPQP GM PGHPPQA AVAPTAPQVP IAQLQPPQNQ LAALMMNPAV QNQIRNHSNA LYSKNLPALA ERFGGNAQNI PPEALAEFRQ KCL NQAKVQ VLSMLQQQQA GGRLAAGLPP QVQAAQVQAM QQQAQALAVA QAQVQAAQQA AAAQAAQQQG GMGQGM

UniProtKB: Spt20-like SEP domain-containing protein

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Macromolecule #3: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 48.541484 KDa
SequenceString: MALSDNNPEE VDGMFLSNLR SDLELEEIWR MLQSGVTSPE DSQPTVSLDA SSSGDKTMSS ESQVALQDDA DDELLVSILA GKVPLSELL ANDNELLQSD GLSSAVCSAD SDQEEDDNND AGYGEEPDPR DVIANWFELC QMMYVSGETG EPSLETTGII E DIVRQQVI ...String:
MALSDNNPEE VDGMFLSNLR SDLELEEIWR MLQSGVTSPE DSQPTVSLDA SSSGDKTMSS ESQVALQDDA DDELLVSILA GKVPLSELL ANDNELLQSD GLSSAVCSAD SDQEEDDNND AGYGEEPDPR DVIANWFELC QMMYVSGETG EPSLETTGII E DIVRQQVI EIIRHDAPKV SRLRTFLAWK DVRKNVKDSD DKGADADIAP GDDPVGAVVP GATVDDTARK TKKSRVGLPW EP ASFYSVE VPEREDEEDE EEEEMNYITL QRLRKADERT KAMTKEEYVT WSEFRQASFT YRKGKRFREW AGFGLVTDSK PSD DIIDIL GFLTFEMVQT LTEEALKIKE QEDLHRERTG ENQSNANNPN GSANSSLSKK RKLTGGGGLF DPPNEGRTPV EPRH IQEAF RRLQQRPKKA RAMLNGTKLQ QRTQLKLF

UniProtKB: Uncharacterized protein

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Macromolecule #4: Transcription initiation factor TFIID subunit 5

MacromoleculeName: Transcription initiation factor TFIID subunit 5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 83.784141 KDa
SequenceString: MSNPAPQAAG QGAAGTPYGN PPPSAGGAGS GSAGASAPST TPSAQNLNQI VTDYLKKKGF TRTEAVFRQE TAHLGPDGRP VNRNDDPGP KKYLKAFLLL KNWIDNNLDI YKFELRKLLW PVFVYSYLEL VSQGYVDDAK HLLETLRSHF EAVHQDQLAL L DTVTLPQH ...String:
MSNPAPQAAG QGAAGTPYGN PPPSAGGAGS GSAGASAPST TPSAQNLNQI VTDYLKKKGF TRTEAVFRQE TAHLGPDGRP VNRNDDPGP KKYLKAFLLL KNWIDNNLDI YKFELRKLLW PVFVYSYLEL VSQGYVDDAK HLLETLRSHF EAVHQDQLAL L DTVTLPQH VSENHTTRLY RENKYRIPLN QSLSGNLFHF LEREADNGGA TIIYILQTHC SVETSARGPI EPYSFEAIYR RA RNLDLDE ADAHEGIPGI FTGVTNRDVL DTSARLKLGP LPMEPELRED VRAELEEEDQ RNPPMDNRPT LVEEFDKKIK REE SADSPS RNDLPLPASR ARDVVMEMQK VRENRDRFVI EGRTGGIGIP VSACMFTFHN TLGTVSCMDF SNDHKLVAVG TMDS YIRVW SLDGKPLKSA LENEKNLKVN NRKLIGHSGP VYGVSFSDSI ANLDRNIYGE GDDKKPDTSS KLLLSCSADG QIRLW SLEI WACLCIYKAH DGPVFRVLWG PHGHYFASAG WDKTVRVFTQ DHASAVRIMV GHDTSISALA WHPNGTYVFS ASDEMD KSI RMWSVITGNC VRIFTGHTHY ITALECAHNG KILASADTGG NIFIWDIEKG TLIKKCRGHG KGGIPSLSFS AESNVLV SG GLDCTVRVWD IELPADPNKN NPLAPSTTQS GQQSDGTTAT GDTIAVGGSA DGRNITVGGQ GTQAGGAGTS GSGVAGAV T GKKKAKEVQI TPDQISAFAT KKTPVLKVRF TRMNLIVAGG CYDPER

UniProtKB: Transcription initiation factor TFIID subunit-like protein

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Macromolecule #5: Transcription initiation factor TFIID subunit 6

MacromoleculeName: Transcription initiation factor TFIID subunit 6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 52.463859 KDa
SequenceString: MATNEGPKLL WNPENVRDVA DALGITLSEE PLRLLAQDVE YRIGQVIVES LRFMRAANRT TLTVQDVSLA LRVLDVEPLY GYESTRPLR YGEASLGPGQ PLFYIDDEEV DFEKVINAPL PKVPRDMTFT AHWLAVEGVQ PSIPQNPTTA ETSSKDLLPK G PGANPALA ...String:
MATNEGPKLL WNPENVRDVA DALGITLSEE PLRLLAQDVE YRIGQVIVES LRFMRAANRT TLTVQDVSLA LRVLDVEPLY GYESTRPLR YGEASLGPGQ PLFYIDDEEV DFEKVINAPL PKVPRDMTFT AHWLAVEGVQ PSIPQNPTTA ETSSKDLLPK G PGANPALA ALAGNDNVSF RPSVKHVISK ELILYFDKIQ AAILDDDPDE EKMRLRQAAL ESVRSDPGLH QLLPYFVNFI TN QVTHHLD DLFILRQMME LAEAVVQNPT LFIDPYASAL AAPVLTCLMS RKLGKNPSSN DEGALGAGNS IDSTLREQYS LRE LAASLL SMIARKYGAS NALLRPKLTR TCLKHFLDPT RPPAVLFGAI SGVAASGGPE AVRVLVLPNL KTFDSAVLQP LREK AGPVA ELEYEMLVGG IVKAVQSIVG DQAALGALTN GINGADLTRE GEQVIEFLGP IVGQRIAQLR NHTLNRSILE VRHLE

UniProtKB: TBP-associated factor 6

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Macromolecule #6: Putative transcription initiation factor

MacromoleculeName: Putative transcription initiation factor / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 28.834281 KDa
SequenceString: MSAATTAPQT NGILPSTTAS GAQTISNTPQ PSTSQPAPSA TPAQSQTPAT TQPQQPTSTT TPSPRPRDAR TLELLLTAQG VTSFEPRVS QLLLDFAYRH TAAVLSDALH LSADPYISHA GARPSASSGA APVNVGEASI TANAVALAIS SRLGYQFRGG G GGYYGGGG ...String:
MSAATTAPQT NGILPSTTAS GAQTISNTPQ PSTSQPAPSA TPAQSQTPAT TQPQQPTSTT TPSPRPRDAR TLELLLTAQG VTSFEPRVS QLLLDFAYRH TAAVLSDALH LSADPYISHA GARPSASSGA APVNVGEASI TANAVALAIS SRLGYQFRGG G GGYYGGGG GGASKDWMLE LARERNKVAL PRVLPSEWGV RLPGERFVLS GVSWGLKDVW AGQEGESEEE SEPEEQGDKM EV DENGKNG EAEDGVEVEG GTMEDVFGDE GLEDEEMGEA

+
Macromolecule #7: Transcription initiation factor TFIID subunit 10

MacromoleculeName: Transcription initiation factor TFIID subunit 10 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 21.417871 KDa
SequenceString: MATNQSIDNS VSQPAAPTAS ANGTSQPAGE TTSMFPGIPS ASVPEPKLPT RKDASLREFL NKMDDYAPII PDAVTNYYMT RAGLPPPPQ TDIRLARLLA LATQKFIADI AADAYQYSRI RASNTNANNP MGSLGAAAGF PIPGQPSNQP GGKEQGRGGP L GIQRPGYG ...String:
MATNQSIDNS VSQPAAPTAS ANGTSQPAGE TTSMFPGIPS ASVPEPKLPT RKDASLREFL NKMDDYAPII PDAVTNYYMT RAGLPPPPQ TDIRLARLLA LATQKFIADI AADAYQYSRI RASNTNANNP MGSLGAAAGF PIPGQPSNQP GGKEQGRGGP L GIQRPGYG GGGQGGSQNR TVLTMEDLGM AVSEFGVNVK RSEFYR

UniProtKB: Transcription initiation factor TFIID subunit 10

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Macromolecule #8: Transcription initiation factor TFIID subunit 12

MacromoleculeName: Transcription initiation factor TFIID subunit 12 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 83.091188 KDa
SequenceString: MNQTPAQGQG QGQPGQGMAN RPPNAPSAAN VAAPGGAPRQ QGTPMYRPEM MRNIPLLSDE EKTKYERGLQ QLWLMHDNTA PGSAENIEA KKKIAEFGRV LLTKLQQRRQ QQQLQQQRQQ QQQQQQQQQQ QQQQQQQVQQ QMQQQAQQPQ TQPQQGQPQQ Q LQQAQVQQ ...String:
MNQTPAQGQG QGQPGQGMAN RPPNAPSAAN VAAPGGAPRQ QGTPMYRPEM MRNIPLLSDE EKTKYERGLQ QLWLMHDNTA PGSAENIEA KKKIAEFGRV LLTKLQQRRQ QQQLQQQRQQ QQQQQQQQQQ QQQQQQQVQQ QMQQQAQQPQ TQPQQGQPQQ Q LQQAQVQQ QVQQQQQIPQ QQQQAPQQQQ QQQQPQQLGQ PGQQVQMMPQ NQQPQVAQNV QPVAPTTVPQ QQPAPATQTA PN QMGAPNS AAAQLRSLPA HIVSHVKDVT FLAPNNVVDK AGWIDQLKLQ YAQKLFVMDS AGRQIKQIAA SLADQSLPPE ERK KLEEKK QTLEKQYNDA ISFANTVRKQ YMQKPAQNGA AVQNAANVAR PQSAQGASAM TQPGAGVGNG TPVAAPVNPM QSSA AAINT AIETAKKQQM AAGRMAGAVN AMAAQVQSPA TPVQAQVSPA TQVHATQQSV PQQPQQIQQA QPTQQPQAQV KIEPG TAPH PAPLNTTMAG PTGVPSAAAP AQNAARMQTP TSATPTSANP NIRPLTHAAA MNLASQQRPG MPAVPTAASG AAPGTP MGI MGAAQQQQQQ QPGHSHAHPP QPTQAPPPHS TNPKLPIPKV LHDKAIQMPQ PVPNIGGAGS GRPTYTSGVM NQPALPK TP AFQLEGEGER VLNKKKLDEL VRQVCGGTAE GQDGNMLTPE VEESVLNMAD AFVDNVLHQA CRNAKERGSK VLEIRDIQ L VLERVYNIRI PGYASEELRT VRKVQPNSNW IKKMSAVQAA KVVPGKGDI

UniProtKB: Transcription initiation factor TFIID subunit 12 domain-containing protein

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Macromolecule #9: Putative transcriptional activator protein

MacromoleculeName: Putative transcriptional activator protein / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 131.755562 KDa
SequenceString: MSIANGNHPW PSPGGPFTNG GPPRTPSAAL DARPHDASQT TSLSLMPEAS SATDINSDDI DHRRALFADL FRRADERLNL LFDERGEYN LSAIESFKRP PSPPAPSLLP PVTDHTPIQE PPRKRAKRVI DEDDYGDDDE EDEDDKPVDK KPPQQTSQLS N NVLLSPSK ...String:
MSIANGNHPW PSPGGPFTNG GPPRTPSAAL DARPHDASQT TSLSLMPEAS SATDINSDDI DHRRALFADL FRRADERLNL LFDERGEYN LSAIESFKRP PSPPAPSLLP PVTDHTPIQE PPRKRAKRVI DEDDYGDDDE EDEDDKPVDK KPPQQTSQLS N NVLLSPSK TSESPAHSSP SPEKQALEKP PETSQQQLGQ GQQQPKSTED ARKELEQARI ATEEIAKRSF HTLFYTLEHD RT AMLEQQQ LEESEKQLQA EMDKAGSGNA QQNQPGHGSL SSANLGASSL TLKHLIARID QKRERVRASD AELRSLMNEV RKN RSKWAS EENIGQEELY EALEKVLSEL KAHTEYSTPF LQRVSKREAP DYYTFIKQPM DLGTMTKKLK SLQYKSKAEF VYDL NLIWD NCLKYNQDMN HPLRRMANGM RKEADKLIPL IPDITIRSRA EVEAEERRKQ NGGVDDDAGD DSDDEPIMSS RGRVA GTKG TSNKSRKAPK EGTPAAGDQK PVVQVNGVQV KGQREGSEAV DGSNGLGTPP VGGSATPAPG LNGHGSSQNA DAMDVD GPS INGMALVGEE AAEQTYEDEA YKIWKQVTKK DRALIAKERY QLFANNKLNV EEPALLRTKA GMRRFLKSRR EAEALGL IK TAYSDSSVTS SAGAKDGVAP KPNETLAEGM EEEADRAVPS YYEPQTIIPD IDPKLQWVED GEGQVINQFE DMLQLVPP G HFTAPSSRLT RRIDANIRQM QETRKLCSKI GVIKQMQIQT HPFVEADIEP HYISGEGPVM AGEVCRSALQ RSVAKIFYH AGFEELQPSA LDCITDIASD YFQKLVRTFN VYREAEKKPA TGAAAERGAR FVPRFTPEEV ILHTLDENGH DIDSLEAYAR DEVERLGNK LAQIHERMKG HLADLLRPAL APDAGADGSG AFNDGSEQFV TGDFAEELGE DFFGFRALGL DKELGLDMLS V PLHLLQSR VRSQYQLQNQ AGAGSAAADL FEPLPPPEPV TKESIMNEIG LVRNFFLAKL HASGDKPLVE DEDLPPKQRK AR PRLGATG KIVSPQKRSP KEQMALAKKK KKLEQQQQQQ QAQQQEQGKG DNKKGAAKTV SLSLPQPAAG QQVNGLTLPS LSA PNPSSQ GGEVNGESIV VNTSLSDTQQ SGDVTTTQGE KDLEMEMEME LEKAAGDEDG MDDGDMVFEA GMMSPESLER

UniProtKB: SAGA complex subunit Spt7

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Macromolecule #10: SCA7 domain-containing protein

MacromoleculeName: SCA7 domain-containing protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 137.082672 KDa
SequenceString: MDSRKDEEAT IKVAPAKGTV LASKPHIWGK LKLKKPPVKQ IKPGNWKEAT LVEDKKKDKD GGAINAASPG PDVNQLDDRA RDTFSNGRP LEDVLELLQC KLCRKGISRT AARDHIAKCL QIKKEKAQRK KEAREARERL KEAAREEEAR RLEEEGAAKG D DDSDDDDG ...String:
MDSRKDEEAT IKVAPAKGTV LASKPHIWGK LKLKKPPVKQ IKPGNWKEAT LVEDKKKDKD GGAINAASPG PDVNQLDDRA RDTFSNGRP LEDVLELLQC KLCRKGISRT AARDHIAKCL QIKKEKAQRK KEAREARERL KEAAREEEAR RLEEEGAAKG D DDSDDDDG KEGAGKAGKK TKKTDVAGKK RKAEGELDKG KAKKKKDEPK PKVAKPKGPV DVERQCGVPL PNGQLCARSL TC KSHSMSA KRAVPGRSLP YDMLLAAYQK KNQAKQQKAA LDANAPLEDE DDLNAGAVDS DEETAAVMSA LANWNPQPVV PQP VFAPLK RQYQLARLHE QLQAATNGGR TNIFKVVGFG AQRLSDGHLG LYDAEDAPGE PDPVGLGSLS RRSSSIVAGE DDTT QQAQI AAVDHESQKE DAPLAEVCKK PCWKPKDRPG SVLDQEYVPM SGRSLSKVTK LKATGRAAAN RQPKQSRRAA SAIPE SSKP LADAPVNTIV SKSSPALPTA TPLYAFANLP TTDLRLQPAE LAIQEMRKRL IAASSSSQSS ISSTGVRAPR GRKRKE DRS MPGIHGSQPM ENIHRATDGV RQTPENMAPT PKESPSDSTV EMRVLELGQL LAQEDSLAKS SPVLPNTPVV SLLGTSQ NG ATNGLKQGVQ HIQRQMATKP TSVSRAISHS PMSSRPLFSA PSPPMMSHSL SPLTRAATPK ALSSSPLVMS MLLPQQPP S HPFVSLPSGS TLSQAMQQSP IMTSMSKSGL TPAATRQPSP GVMPHSPPPR QGPGRPPKPR PADPVSPVNP TRSPNLPNA SYLTTLGDLS TGMQPPQLPH MPQQQVPRVK LNQMRNQALA STFSPRPSYL PSQRLDPIRE QAIKRAAERA AGLRMAQAQR QPQMQVHMN VQAQRTPQLQ ARTSPPTASP AETQGQMLSA RAQAEDQGQN RLRMGQPQAN TQLMRIDGPG MAAGMRQPIG N SQMDTMQP QRQVMNTPVV PAPQSSSPPS AERSSSQPQP VRDQIDSASV QAEAAQDETM NLDNDEMDDI LREVKAQAER EA ERQMAAL SRTRPQAQQQ AQNLRSTQNQ SQMHALSPVA SESSSPSLVM SASLPGLNEV NVRATVAAAA KKVAGSISPV ANI SSAATA LSSPLDCRPY TTTSIPRPRL ATTAPVPLMG ATGPPAASGT TQNIGSFHVQ QHRQRRQQQQ LLQQHAQQHQ QRLQ QAAVH NNQQPFRFTY PAESMAAAVA IAGAHNLRPV VPLPPQAYLL SASQGGGRSG SRGSNIRNGS QARTQEQG

UniProtKB: SCA7 domain-containing protein

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Macromolecule #11: Putative transcriptional coactivator HFI1 protein

MacromoleculeName: Putative transcriptional coactivator HFI1 protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 52.133355 KDa
SequenceString: MVDINPAALS RPSIGLSTPS FPGKSGSINV SVPSSASASA SASSAHSKPQ KTSLVPPRID LEPIYTALKA AIGAEQWPVY KETLTNFLI GRLNQAELSE RIDPILASQD GQKEHLHNQL IAAIYANVTR EMPDPGLAPW VSANDKPTTA QTKPVTGDAN E RRLKGEVM ...String:
MVDINPAALS RPSIGLSTPS FPGKSGSINV SVPSSASASA SASSAHSKPQ KTSLVPPRID LEPIYTALKA AIGAEQWPVY KETLTNFLI GRLNQAELSE RIDPILASQD GQKEHLHNQL IAAIYANVTR EMPDPGLAPW VSANDKPTTA QTKPVTGDAN E RRLKGEVM QLPARDRRRI KELAHNDFDP YDALANVLME HARAKPPKIA EPPASAGGLK MNFDLEIRKR YAQPLAVESG EF PDTSNIE SRMLPFCYEA GLSSGHAPDA AQFMSIATET FIKEVMSAIF SRTRSNGPGD SGSAGFGLGS GWIQTHRYRK QLA KEEEAF QRGEISRDKM GLLPVEAKAA SERGPLGMAD VRLALEMGDC GLAGFPVIVK SVIYGWREGE LENWEDYTYV DPSR PDGIA IPSRGDGDVE MGGVGVNGVV KGEPEPQQQP LANGVHDVDA MDIDGVSVAG GAANEIWWEG AEPETSDLLN AVLDS CLAV N

UniProtKB: Putative transcriptional coactivator HFI1 protein

+
Macromolecule #12: Transcriptional adapter 2

MacromoleculeName: Transcriptional adapter 2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 58.86607 KDa
SequenceString: MGVIRKKIAG RSGEGGVKYI CDVCSVDITS TVRIRCADPA CHDYDLCVPC FANGSSSNAH KPATHSFRVI EQNSFPIFDP DWGADEELL LLEGAEIYGL GSWADIADHI GGYRTKDEVR DHYLKVYIES PNFPLPERCS PYDLELPNSI SREEFQARKK R RIEERREA ...String:
MGVIRKKIAG RSGEGGVKYI CDVCSVDITS TVRIRCADPA CHDYDLCVPC FANGSSSNAH KPATHSFRVI EQNSFPIFDP DWGADEELL LLEGAEIYGL GSWADIADHI GGYRTKDEVR DHYLKVYIES PNFPLPERCS PYDLELPNSI SREEFQARKK R RIEERREA AKNAPPPQPK TKPTASIPAC HEIQGYMPGR LEFETEYCNE AEEAVQLMSF DPGDGINPRT GELEPEMELK LT VMEIYNN RLTQRVERKK VIFEHNLLEY RENTKAEKKR SREERELLNK AKPFARMMNR HDFEQFCQGL IDELNLRQAI AQL QEWRSM RIGDLKSGEK YEQEKALRIQ KSMPMGSLDR ERLATSQRGK NQQQPEPPSG AALLVAPELP ARYKEPIIDA NGFP RPDAN RLTVDGGSVV VANGTSQPKP KYVPPPVPGV QPMNLTQDNA PDLHLLTPEE IKLCETLRIQ PKPYIMIKEQ ILKEA VKGN GSLKKKQAKE ICRLDSQKGG RIFDFMVNAG WVVKA

UniProtKB: Transcriptional adapter 2

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Macromolecule #13: histone acetyltransferase

MacromoleculeName: histone acetyltransferase / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: histone acetyltransferase
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 46.983691 KDa
SequenceString: MTSENGKRKA TEEPGSPSAA KRIKQEPSET DKDKKEENKD KKTVAKPPPI PYPEKPAVIE ERNGEIEFRV VNNDGERESL IILTGLKCI FQKQLPKMPK DYIARLVYDR THLSIAIVKK PLEVVGGITY RPFKNRKFAE IVFCAISSDQ QVKGYGAHLM S HLKDYVKA ...String:
MTSENGKRKA TEEPGSPSAA KRIKQEPSET DKDKKEENKD KKTVAKPPPI PYPEKPAVIE ERNGEIEFRV VNNDGERESL IILTGLKCI FQKQLPKMPK DYIARLVYDR THLSIAIVKK PLEVVGGITY RPFKNRKFAE IVFCAISSDQ QVKGYGAHLM S HLKDYVKA TTNIEHFLTY ADNYAIGYFK KQGFTKEITL DKSVWMGYIK DYEGGTIMQC TMIPRIRYLE QGRMLLKQKE CV QAKIRAF SKSHIVHPPP KQWRNGNVTP IDPLSIDAIR ESGWSPDMDE LARQPRHGPN YNQLLHLLND MQNHASSWPF LVP VNKDEV VDYYDIIKEP MDLSTMESKL EADQYQTPED FIRDAKLIFD NCRKYNNENT PYAKSANKLE KFMWQQIRQI PEWS HLEPS

UniProtKB: histone acetyltransferase

+
Macromolecule #14: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 79.972164 KDa
SequenceString: MAPSSSQKGT GKKAGSAASA IRSHQQHSQQ QRSRNTTPGP AGIPPTSSTS VSVSVASLPP IETVETETLD LRFDTFRNVT FEDLVDPAA ASALIPDSKS LDGIISRLQR LADIIEKRGA NCDRGMRLLA QTRRQRLDEL AVERGREEER RQAEADDEKK A NKKKRKAD ...String:
MAPSSSQKGT GKKAGSAASA IRSHQQHSQQ QRSRNTTPGP AGIPPTSSTS VSVSVASLPP IETVETETLD LRFDTFRNVT FEDLVDPAA ASALIPDSKS LDGIISRLQR LADIIEKRGA NCDRGMRLLA QTRRQRLDEL AVERGREEER RQAEADDEKK A NKKKRKAD SLAPGAVNTE RSSPNRESTS KARKLDRDDT ASSSLSPAAA GTPSNMDVDD KDKEKDKDKT KAEADSDSDS DD GAPPPPP RPPANVFGDD PSTFPDPTVY EIRPVLPGMS EEEIKEIYSV ASYPKSDLAH LTCGEPPDRD FSNSKPTNQI NFS TFSSYI EPYFRPFTEE DLAFLRERGD RVTPFIMPKR GKKHYTEIWA EEDGAMAIDS SPPGGRDRLP PNQARGSIDN MDDE VAETD KLSVGPLLTR LLQAMRPENR APTADDKSGP NGTSGDGDGP QQSFDFNDST AAPNPSQSQP NGVNGVAATT NGDSQ QQQQ QQQHQSLPPA TFMPESNTEA WKKATHPKLD YNQVDERIKQ ELRHIGFLPL PPSSATDPSN SSASVTPAAP PPPGTT ATT AEYDGHYDDE VAARLRVLQA RLREQILLNG ARKARLTELV KERMAYQEYQ TILEDLDAQV NAAYLKRTRT MGKKPKK NR PGQHSSGSAA AAAAAAAGMA RPGIGDLART LMDRRRRWIE QIGAVFDDEG ITKCPRVEDG DTSIFGREIM AELIKREK E AWDEEVEEE

UniProtKB: Uncharacterized protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Sugar embeddingMaterial: HEPES Buffer
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 68.9 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: OTHER / Software - Name: RELION / Number images used: 3566416
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: PROJECTION MATCHING

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