EMDB/PDB/SASBDBから引用されている文献のデータベース

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構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
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IF	>30 >20 >10 >5 all

タイトル	Structures of rotary ATP synthase from during proton powered ATP synthesis.
ジャーナル・号・ページ	Sci Adv, Vol. 11, Issue 42, Page eadx8771, Year 2025
掲載日	2025年10月17日
著者	Atsuki Nakano / Jun-Ichi Kishikawa / Nishida Yui / Kyosuke Sugawara / Yuto Kan / Christoph Gerle / Hideki Shigematsu / Kaoru Mitsuoka / Ken Yokoyama /
PubMed 要旨	ATP synthases are rotary molecular machines that use the proton motive force to rotate the central rotor complex relative to the surrounding stator apparatus, thereby coupling the ATP synthesis. We ...ATP synthases are rotary molecular machines that use the proton motive force to rotate the central rotor complex relative to the surrounding stator apparatus, thereby coupling the ATP synthesis. We reconstituted the V/A-ATPase into liposomes and performed structural analysis using cryo-EM under conditions where the proton motive force was applied in the presence of ADP and Pi. ATP molecules were bound at two of the three catalytic sites of V/A-ATPase, confirming that the structure represents a state adopted during ATP synthesis. In this structure, the catalytic site closes upon binding of ADP and Pi through an induced fit mechanism. Multiple structures were obtained where the membrane-embedded rotor ring was in a different position relative to the stator. By comparing these structures, we found that torsion occurs in both the central rotor and the peripheral stator during 31° rotation of rotor ring. These structural snapshots of V/A-ATPase provide crucial insights into the mechanism of rotary catalysis of ATP synthesis.
リンク	Sci Adv / PubMed:41105777 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 4.67 Å
構造データ	63904 9u6f EMDB-63904, PDB-9u6f: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes, state1 手法: EM (単粒子) / 解像度: 2.2 Å 63905 9u6g EMDB-63905, PDB-9u6g: Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes, state1 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-63906: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes, state2 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-63907: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes, state3 手法: EM (単粒子) / 解像度: 2.6 Å 63908 9u6h EMDB-63908, PDB-9u6h: Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes, state2 手法: EM (単粒子) / 解像度: 3.0 Å 63909 9u6i EMDB-63909, PDB-9u6i: Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes, state3 手法: EM (単粒子) / 解像度: 3.0 Å 63910 9u6j EMDB-63910, PDB-9u6j: Cryo-EM structure of the Vo domain of V/A-ATPase under pmf condition, State3A 手法: EM (単粒子) / 解像度: 4.02 Å 63911 9u6k EMDB-63911, PDB-9u6k: Cryo-EM structure of the Vo domain of V/A-ATPase under pmf condition, State3B 手法: EM (単粒子) / 解像度: 4.1 Å 63912 9u6l EMDB-63912, PDB-9u6l: Cryo-EM structure of the Vo domain of V/A-ATPase under pmf condition, State3C 手法: EM (単粒子) / 解像度: 4.67 Å 63913 9u6m EMDB-63913, PDB-9u6m: Cryo-EM structure of the Vo domain of V/A-ATPase under pmf condition, state3D 手法: EM (単粒子) / 解像度: 4.64 Å 63914 9u6n EMDB-63914, PDB-9u6n: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under no pmf condition, state1W 手法: EM (単粒子) / 解像度: 2.85 Å 63915 9u6o EMDB-63915, PDB-9u6o: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under no pmf condition, state1M 手法: EM (単粒子) / 解像度: 2.8 Å 63916 9u6p EMDB-63916, PDB-9u6p: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under no pmf condition, state1N 手法: EM (単粒子) / 解像度: 2.85 Å EMDB-63917: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under no pmf condition, state2 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-63918: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under no pmf condition, state3 手法: EM (単粒子) / 解像度: 3.0 Å 63919 9u6q EMDB-63919, PDB-9u6q: Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes under no pmf condition,state1 手法: EM (単粒子) / 解像度: 3.6 Å 63922 9u6t EMDB-63922, PDB-9u6t: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under pmf condition, state1W 手法: EM (単粒子) / 解像度: 2.9 Å 63923 9u6u EMDB-63923, PDB-9u6u: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under pmf condition, state1M 手法: EM (単粒子) / 解像度: 2.9 Å 63924 9u6v EMDB-63924, PDB-9u6v: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under pmf condition, state1N 手法: EM (単粒子) / 解像度: 3.0 Å 63925 9u6w EMDB-63925, PDB-9u6w: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under pmf condition, state3W 手法: EM (単粒子) / 解像度: 3.1 Å 63926 9u6x EMDB-63926, PDB-9u6x: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under pmf condition, state3M 手法: EM (単粒子) / 解像度: 3.0 Å 63927 9u6y EMDB-63927, PDB-9u6y: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under pmf condition, state3N 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-63928: Cryo-EM structure of the V1 domain of V/A-ATPase in liposomes under pmf condition,state2 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-63929: Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes under pmf condition,state1 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-63930: Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes under pmf condition,state2 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-PO4: PHOSPHATE ION / ホスファ-ト
由来	thermus thermophilus hb8 (バクテリア)
キーワード	MOTOR PROTEIN / ATP synthase / V ATPase / V1 ATPase