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TitleCryo-EM structure of Chlamydomonas reinhardtii Photosystem I complexed with cytochrome c.
Journal, issue, pagesNat Commun, Vol. 17, Issue 1, Year 2026
Publish dateMar 27, 2026
AuthorsYu Ogawa / Gyana Prakash Mahapatra / Yuval Milrad / Michelle Schimpf / Genji Kurisu / Michael Hippler / Jan Michael Schuller /
PubMed AbstractPhotosynthetic electron transfer relies on small soluble carriers that shuttle electrons between the cytochrome b₆f complex and Photosystem I (PSI). While copper-containing plastocyanin (Pc) serves ...Photosynthetic electron transfer relies on small soluble carriers that shuttle electrons between the cytochrome b₆f complex and Photosystem I (PSI). While copper-containing plastocyanin (Pc) serves this role in plants, the heme protein cytochrome c₆ (Cyt c₆) is also employed in algae and cyanobacteria. Here, we present a cryo-electron microscopy structure of a Cyt c₆:PSI complex from Chlamydomonas reinhardtii. We observe that the heme group of Cyt c₆ is positioned ~11 Å away from P700, stabilized by extensive contacts involving a N-terminal helix-loop-helix motif of PSAF, characteristic of eukaryotic PSI. Notably, the algal Cyt c₆ also retains an arginine residue (R66) which is crucial for cyanobacterial donor:PSI reactions. Our structure reveals the previously uncharacterized interactions involving this residue; it can form a putative electrostatic contact with PsaB-D623 while also contributing to a tri-planar π(cation)-π interactions with adjacent residues. Our findings provide a structural framework for understanding the mechanism and evolution of donor:PSI interactions.
External linksNat Commun / PubMed:41896549 / PubMed Central
MethodsEM (single particle)
Resolution1.83 - 2.06 Å
Structure data

54803

9se6

EMDB-54803, PDB-9se6:
Structure of Photosystem I from Chlamydomonas reinhardtii at 1.83 A resolution
Method: EM (single particle) / Resolution: 1.83 Å

54804

9se7

EMDB-54804, PDB-9se7:
Structure of Cytochrome C6 bound Photosystem I from Chlamydomonas reinhardtii at 2.07 A resolution
Method: EM (single particle) / Resolution: 2.06 Å

Chemicals

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-CHL:
CHLOROPHYLL B

ChemComp-BCR:
BETA-CAROTENE

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-CL0:
CHLOROPHYLL A ISOMER

ChemComp-PQN:
PHYLLOQUINONE

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-HOH:
WATER

ChemComp-HEC:
HEME C

Source
  • chlamydomonas reinhardtii (plant)
KeywordsPHOTOSYNTHESIS / Photosystem I / Chlamydomonas reinhardtii / CryoEM / Cytochrome C6

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