EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of TACO1-mediated efficient mitochondrial translation.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2026年2月9日
著者	Shuhui Wang / Michele Brischigliaro / Yuekang Zhang / Chunxiang Wu / Wei Zheng / Antoni Barrientos / Yong Xiong /
PubMed 要旨	Translation elongation is a universally conserved step in protein synthesis, relying on elongation factors that engage the ribosomal L7/L12 stalk to mediate aminoacyl-tRNA delivery, accommodation, ...Translation elongation is a universally conserved step in protein synthesis, relying on elongation factors that engage the ribosomal L7/L12 stalk to mediate aminoacyl-tRNA delivery, accommodation, and ribosomal translocation. Using in organello cryo-electron microscopy, we reveal how the mitochondrial translation accelerator TACO1 promotes efficient elongation on human mitoribosomes. TACO1 binds the mitoribosomal region typically bound by elongation factor Tu (mtEF-Tu), bridging the large and small subunits via contacts with 16S rRNA, bL12m, A-site tRNA, and uS12m. While active throughout elongation, TACO1 is especially critical when translating polyproline motifs. Its absence prolongs mtEF-Tu residence in A/T states, causes persistent mitoribosomal stalling and premature subunit dissociation. Structural analyses indicate that TACO1 competes with mtEF-Tu for mitoribosome binding, stabilizes A-site tRNA, and enhances peptidyl transfer through a mechanism distinct from EF-P and eIF5A. These findings suggest that bacterial TACO1 orthologs may serve analogous roles, highlighting an evolutionarily conserved strategy for maintaining elongation efficiency during challenging translation events.
リンク	Nat Commun / PubMed:41663403
手法	EM (単粒子)
解像度	2.46 - 3.34 Å
構造データ	70592 9olf EMDB-70592, PDB-9olf: Membrane-associated human mitoribosome in complex with TACO1 手法: EM (単粒子) / 解像度: 2.46 Å EMDB-70620: In situ mitoribosome focused on the mtSSU 手法: EM (単粒子) / 解像度: 2.65 Å EMDB-70621: Consensus map of the mitoribosome complexed with TACO1 手法: EM (単粒子) / 解像度: 2.48 Å 71623 9pg8 EMDB-71623, PDB-9pg8: In situ structure of the human mitoribosome in the P-E state 手法: EM (単粒子) / 解像度: 3.06 Å 71630 9pgf EMDB-71630, PDB-9pgf: In situ structure of the human mitoribosome in the P state 手法: EM (単粒子) / 解像度: 2.93 Å 71633 9pgi EMDB-71633, PDB-9pgi: In situ structure of the human mitoribosome in the A-P-E state with TACO1 手法: EM (単粒子) / 解像度: 3.02 Å 71634 9pgl EMDB-71634, PDB-9pgl: In situ structure of the human mitoribosome in the A-P state with TACO1 手法: EM (単粒子) / 解像度: 2.9 Å 71635 9pgm EMDB-71635, PDB-9pgm: In situ structure of the human mitoribosome in the P-E state from TACO1-knockout cells 手法: EM (単粒子) / 解像度: 3.18 Å 71797 9pr4 EMDB-71797, PDB-9pr4: In Situ Structure of the Human Mitochondrial Large Subunit 39S in Complex with TACO1 手法: EM (単粒子) / 解像度: 2.77 Å 71802 9pra EMDB-71802, PDB-9pra: In Situ Structure of the Human Mitoribosome Large Subunit 39S in Complex with EF-Tu 手法: EM (単粒子) / 解像度: 2.83 Å 71809 9prd EMDB-71809, PDB-9prd: In situ structure of the human mitoribosome in the P state from TACO1-knockout cells 手法: EM (単粒子) / 解像度: 3.31 Å 71811 9prq EMDB-71811, PDB-9prq: In situ structure of human mitoribosome in the A/T-P state from TACO1-knockout cells 手法: EM (単粒子) / 解像度: 3.34 Å 71815 9prx EMDB-71815, PDB-9prx: In situ structure of the human mitoribosome in the A-P state from TACO1-knockout cells 手法: EM (単粒子) / 解像度: 3.23 Å 71818 9ps0 EMDB-71818, PDB-9ps0: In situ structure of the human mitoribosome in the A/P-P/E state from TACO1-knockout cells 手法: EM (単粒子) / 解像度: 3.29 Å 71825 9ps7 EMDB-71825, PDB-9ps7: In situ structure of the human mitoribosome in the A/T-P-E state from TACO1-knockout cells 手法: EM (単粒子) / 解像度: 3.08 Å 71828 9psi EMDB-71828, PDB-9psi: In situ structure of the human mitoribosome in the A-P-E state from TACO1-knockout cells 手法: EM (単粒子) / 解像度: 3.12 Å 71829 9psm EMDB-71829, PDB-9psm: In situ structure of the human mitoribosome in the A/P-P/E state 手法: EM (単粒子) / 解像度: 2.98 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PUT: 1,4-DIAMINOBUTANE / プトレシン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-SPM: SPERMINE / スペルミン ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-VAL*: VALINE / バリン
由来	homo sapiens (ヒト)
キーワード	RIBOSOME / mitochondria / mitoribosome / inner membrane / mitochondrial ribosome