Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Visualizing insecticide control of insect TRP channel function and assembly.
Journal, issue, pages	Nat Commun, Vol. 17, Issue 1, Page 595, Year 2025
Publish date	Dec 14, 2025
Authors	Justin G Fedor / Ramani Kandasamy / Cheon-Gyu Park / Yang Suo / Martin Weisel / Nancy B Rankl / Alexandre Nesterov / Seok-Yong Lee /
PubMed Abstract	Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against ...Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against underutilized targets. Nanchung (Nan) and Inactive (Iav), the transient receptor potential vanilloid-type (TRPV) channels in insects, likely form a heteromeric channel (Nan-Iav) and are localized in mechanosensory chordotonal organs which confer gravitaxis, hearing and proprioception. Several insecticides, such as afidopyropen (AP), target Nan-Iav through unknown mechanisms. Effective against piercing-sucking (hemipteran) insects, AP disrupts chordotonal functions preventing feeding. AP can bind to Nan alone, but only Nan-Iav exhibits channel activity with agonists including endogenous nicotinamide (NAM). Despite its importance as an insecticide target, much is unknown about Nan-Iav, such as channel assembly, modulator binding sites, and Ca-dependent regulation, hampering further insecticide development. Here we present the cryo-electron microscopy structures of hemipteran Nan-Iav with calmodulin bound in the apo state and with AP and NAM bound to cytosolic ankyrin repeat domain (ARD) interfaces. Unexpectedly, we found that Nan alone can form a pentamer, stabilized through AP-mediated ARD interactions. Our study provides molecular insights into insecticide and agonist interactions with Nan-Iav, highlighting the importance of the ARD on channel function and assembly, while also probing regulation by Ca.
External links	Nat Commun / PubMed:41392056 / PubMed Central
Methods	EM (single particle)
Resolution	2.49 - 3.28 Å
Structure data	49844 9nvn EMDB-49844, PDB-9nvn: Structure of Nanchung-Inactive-Calmodulin in apo state Method: EM (single particle) / Resolution: 2.89 Å 49845 9nvo EMDB-49845, PDB-9nvo: Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide Method: EM (single particle) / Resolution: 2.49 Å 49846 9nvp EMDB-49846, PDB-9nvp: Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide, EDTA Method: EM (single particle) / Resolution: 2.85 Å 49847 9nvq EMDB-49847, PDB-9nvq: Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen and calcium Method: EM (single particle) / Resolution: 3.01 Å 49848 9nvr EMDB-49848, PDB-9nvr: Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen, EDTA Method: EM (single particle) / Resolution: 3.13 Å 49849 9nvs EMDB-49849, PDB-9nvs: Structure of a pentameric Nanchung in complex with Afidopyropen Method: EM (single particle) / Resolution: 3.28 Å
Chemicals	ChemComp-6OU: [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate / phospholipidYM ChemComp-LBN: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / phospholipidYM ChemComp-CA: Unknown entry ChemComp-D39: (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid ChemComp-NCA: NICOTINAMIDE / medicationYM PDB-1b32*: OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KMK
Source	homo sapiens (human) halyomorpha halys (brown marmorated stink bug)
Keywords	TRANSPORT PROTEIN / Membrane protein / membrane channel