EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis of SAM-AMP synthesis and degradation in the type III-B CRISPR-Cas system.
ジャーナル・号・ページ	Nat Chem Biol, Year 2025
掲載日	2025年11月21日
著者	Benzhen Duan / Xiaohui Jin / Xiaoman An / Yang Xiao / Qianxi Yang / Hongyu Zhao / Yunxiao Huang / Jingwen Wang / Qian Wang / Fenglei Du / Lu Lu / Lei Sun / Zhenguo Chen / Baoyu Zhao /
PubMed 要旨	Upon sensing nonself target RNA, the CorA-associated type III-B CRISPR-Cas system catalyzes S-adenosyl methionine (SAM) and ATP to synthesize SAM-AMP, which activates the effector CorA and triggers ...Upon sensing nonself target RNA, the CorA-associated type III-B CRISPR-Cas system catalyzes S-adenosyl methionine (SAM) and ATP to synthesize SAM-AMP, which activates the effector CorA and triggers immune responses. SAM-AMP can be degraded by NrN and SAM lyase, potentially deactivating the system. Here we find that the type III-B effector complex from Bacteroides fragilis uses a specific mechanism to recognize nonself target RNA and synthesize SAM-AMP. The 3' anti-tag of nonself target RNA induces conformational changes in the Cmr2 subunit, triggering SAM-AMP synthesis independently of the stalk loop of Cmr3 subunit. SAM-AMP binding induces NrN to transit from an open to a closed conformation, enabling hydrolysis of the 3'-5' phosphodiester bond. SAM lyase forms a triangular trimer that specifically degrades SAM-AMP into 5'-methylthioadenosine-AMP and homoserine lactone. These findings unveil unique mechanisms for SAM-AMP synthesis and degradation and provide deeper insights into the molecular basis of type III CRISPR-Cas signaling.
リンク	Nat Chem Biol / PubMed:41272318
手法	EM (単粒子) / X線回折
解像度	1.28 - 2.97 Å
構造データ	63293 9lq5 EMDB-63293, PDB-9lq5: Cryo-EM structure of Apo type III-B CRISPR-Cas effector complex 手法: EM (単粒子) / 解像度: 2.97 Å 63294 9lq6 EMDB-63294, PDB-9lq6: Cryo-EM structure of adTR-bound type III-B CRISPR-Cas effector complex 手法: EM (単粒子) / 解像度: 2.94 Å 63295 9lq7 EMDB-63295, PDB-9lq7: Cryo-EM structure of NTR-bound type III-B CRISPR-Cas effector complex 手法: EM (単粒子) / 解像度: 2.81 Å 63296 9lq8 EMDB-63296, PDB-9lq8: Cryo-EM structure of CTR-bound type III-B CRISPR-Cas effector complex 手法: EM (単粒子) / 解像度: 2.85 Å PDB-9lq9: Crysral structure of NrN 手法: X-RAY DIFFRACTION / 解像度: 2.38 Å PDB-9lqa: Crystal structure of NrN E21A in complex with SAM-AMP 手法: X-RAY DIFFRACTION / 解像度: 1.91 Å PDB-9lqb: Crystal structure of NrN E21A in complex with SAH-AMP 手法: X-RAY DIFFRACTION / 解像度: 1.93 Å PDB-9lqc: Crystal structure of NrN D119A in complex with SAH-AMP 手法: X-RAY DIFFRACTION / 解像度: 2.37 Å PDB-9lqd: Crystal structure of SAM lyase 手法: X-RAY DIFFRACTION / 解像度: 2.16 Å PDB-9lqe: Crystal structure of SAM lyase in complex with SAH 手法: X-RAY DIFFRACTION / 解像度: 1.94 Å PDB-9lqf: Crystal structure of SAM lyase in complex with SAH-AMP 手法: X-RAY DIFFRACTION / 解像度: 1.59 Å PDB-9lqg: Crystal structure of SAM lyase in complex with MTA-AMP 手法: X-RAY DIFFRACTION / 解像度: 1.28 Å PDB-9lqh: Crystal structure of SAM lyase in complex with sinefungin-AMP 手法: X-RAY DIFFRACTION / 解像度: 1.47 Å
化合物	ChemComp-MN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-SAM: S-ADENOSYLMETHIONINE ChemComp-HOH: WATER PDB-1el3: HUMAN ALDOSE REDUCTASE COMPLEXED WITH IDD384 INHIBITOR ChemComp-TRS: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH緩衝剤YM PDB-1el0: SOLUTION STRUCTURE OF THE HUMAN CC CHEMOKINE, I-309 ChemComp-MLT: D-MALATE ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE ChemComp-GOL: GLYCEROL PDB-1ijb: The von Willebrand Factor mutant (I546V) A1 domain PDB-1el1: X-RAY CRYSTAL STRUCTURE ANALYSIS OF CANINE MILK LYSOZYME (HOLO-TYPE)
由来	bacteroides fragilis (バクテリア) clostridium botulinum (strain hall / atcc 3502 / nctc 13319 / type a) (ボツリヌス菌)
キーワード	IMMUNE SYSTEM/RNA / CRISPR-Cas complex / adaptive immunity / RNA degradation / second messenger / IMMUNE SYSTEM-RNA complex / IMMUNE SYSTEM / enzyme / phosphodiesterase / CRISPR-Cas system / SAM