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Structure paper

TitleStructural basis for the interaction between the bacterial cell division proteins FtsZ and ZapA.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 5985, Year 2025
Publish dateJul 1, 2025
AuthorsJunso Fujita / Kazuki Kasai / Kota Hibino / Gota Kagoshima / Natsuki Kamimura / Shungo Tobita / Yuki Kato / Ryo Uehara / Keiichi Namba / Takayuki Uchihashi / Hiroyoshi Matsumura /
PubMed AbstractCell division in most bacteria is regulated by the tubulin homolog FtsZ as well as ZapA, a FtsZ-associated protein. However, how FtsZ and ZapA function coordinately has remained elusive. Here we ...Cell division in most bacteria is regulated by the tubulin homolog FtsZ as well as ZapA, a FtsZ-associated protein. However, how FtsZ and ZapA function coordinately has remained elusive. Here we report the cryo-electron microscopy structure of the ZapA-FtsZ complex at 2.73 Å resolution. The complex forms an asymmetric ladder-like structure, in which the double antiparallel FtsZ protofilament on one side and a single protofilament on the other side are tethered by ZapA tetramers. In the complex, the extensive interactions of FtsZ with ZapA cause a structural change of the FtsZ protofilament, and the formation of the double FtsZ protofilament increases electrostatic repulsion. High-speed atomic force microscopy analysis revealed cooperative interactions of ZapA with FtsZ at a molecular level. Our findings not only provide a structural basis for the interaction between FtsZ and ZapA but also shed light on how ZapA binds to FtsZ protofilaments without disturbing FtsZ dynamics to promote cell division.
External linksNat Commun / PubMed:40593603 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution1.8 - 2.73 Å
Structure data

60837

9isk

EMDB-60837, PDB-9isk:
Cryo-EM structure of KpFtsZ-ZapA complex
Method: EM (helical sym.) / Resolution: 2.73 Å

PDB-9isj:
Crystal structure of Klebsiella pneumoniae ZapA
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

Source
  • Klebsiella pneumoniae (bacteria)
  • klebsiella pneumoniae subsp. pneumoniae mgh 78578 (bacteria)
  • klebsiella pneumoniae 342 (bacteria)
  • klebsiella pneumoniae (strain 342) (bacteria)
KeywordsCELL CYCLE / Bacterial cell division / divisome / FtsZ / ZapA

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