EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Pseudouridine synthase PUS1 and initiation factor mtIF2 are human mitoribosomal small subunit assembly factors.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Year 2026
掲載日	2026年4月21日
著者	Vivek Singh / Dmitrii Shiriaev / Lorina Bilalli / Anas Khawaja / Joanna Rorbach /
PubMed 要旨	Assembly of the mitochondrial ribosome (mitoribosome) is a crucial step in mitochondrial gene expression. This process facilitates mitochondrial translation, which produces essential subunits of the ...Assembly of the mitochondrial ribosome (mitoribosome) is a crucial step in mitochondrial gene expression. This process facilitates mitochondrial translation, which produces essential subunits of the oxidative phosphorylation machinery-the cell's primary energy-producing machinery. Disruptions in mitoribosome assembly can lead to severe human diseases. Given its fundamental importance, detailed structural analysis of mitoribosome assembly pathways is essential for advancing our understanding of mitochondrial function in both health and disease. In this study, we characterize twelve distinct assembly states of the mitoribosomal small subunit (mtSSU) isolated from human cells. Our findings reveal the intricate details of the final maturation stages of the mtSSU platform, decoding center, and the 3'-end of 12S rRNA. This process is governed by coordinated actions of assembly factors that ensure precise, stepwise rRNA folding and the integration of mitoribosomal proteins into the developing subunit. Our approach identifies pseudouridine synthase PUS1 and initiation factor mtIF2 as assembly factors, expanding their known roles beyond mt-tRNA maturation and translation, respectively. In addition, the identified assembly intermediates provide insight into the modular nature of mtSSU biogenesis in mitochondria and further link late-stage assembly to the acquisition of translational competence.
リンク	Nat Commun / PubMed:42342677 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 4.6 Å
構造データ	51873 9h51 EMDB-51873, PDB-9h51: Assembly intermediate of human mitochondrial ribosome small subunit in complex with PUS1, METTL15 and RBFA(IN conformation) (state M1) 手法: EM (単粒子) / 解像度: 3.1 Å 51874 EMDB 未公開エントリ 9h52 EMDB-51874, PDB-9h52: Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1, ERAL1, METTL17, MCAT and TFB1M (state N1) 手法: EM (単粒子) / 解像度: 3.9 Å 51876 9h54 EMDB-51876, PDB-9h54: Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and partial RBFA (state N2) 手法: EM (単粒子) / 解像度: 3.0 Å 51877 9h55 EMDB-51877, PDB-9h55: Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and TFB1M (state N3) 手法: EM (単粒子) / 解像度: 3.4 Å 54129 9ror EMDB-54129, PDB-9ror: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and RBFA (Outward conformation) (State M3) 手法: EM (単粒子) / 解像度: 3.64 Å 54130 9rot EMDB-54130, PDB-9rot: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15, RBFA, and mtIF2 (State M2.1) 手法: EM (単粒子) / 解像度: 4.3 Å 54131 9rov EMDB-54131, PDB-9rov: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and RBFA (Inward conformation) (State M2) 手法: EM (単粒子) / 解像度: 3.1 Å 54163 9rpf EMDB-54163, PDB-9rpf: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and mS37 (State M4) 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-57663: human mitochondrial ribosome small subunit: Initiation complex with IF3 (State I2) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-57667: Human mitochondrial small ribosomal subunit: Initiation complex with IF2 and IF3 (State I3) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-57668: Human mitochondrial small ribosomal subunit: Initiation complex with IF2 and tRNA (state I4) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-57669: Human mitochondrial small ribosomal subunit: Assembly intermediate with IF3 and RBFA-out (state I1) 手法: EM (単粒子) / 解像度: 4.3 Å
化合物	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM ChemComp-SPM: SPERMINE ChemComp-MG: Unknown entry ChemComp-K: Unknown entry ChemComp-ZN: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-SF4*: IRON/SULFUR CLUSTER
由来	homo sapiens (ヒト)
キーワード	RIBOSOME / mitochondria / small subunit / assembly / NOA1 / mitochondrial gene expression / mitoribosome / mitoribosome assembly factors / cryoEM