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Open data
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Basic information
| Entry | ![]() | |||||||||
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| Title | Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15, RBFA, and mtIF2 (State M2.1) | |||||||||
Map data | Consensus map M15-RBFA-IF2 State M2.1 | |||||||||
Sample |
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Keywords | mitochondrial gene expression / mitoribosome / mitoribosome assembly factors / cryoEM / RIBOSOME | |||||||||
| Biological species | Homo sapiens (human) | |||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 4.3 Å | |||||||||
Authors | Khawaja A / Singh V / Shiriaev DI / Rorbach J | |||||||||
| Funding support | Sweden, 1 items
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Citation | Journal: Nat Commun / Year: 2026 Title: Pseudouridine synthase PUS1 and initiation factor mtIF2 are human mitoribosomal small subunit assembly factors Authors: Singh V / Shiriaev D / Bilalli L / Khawaja A / Rorbach J | |||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Header (meta data) | emd-54130-v30.xml emd-54130.xml | 56.1 KB 56.1 KB | Display Display | EMDB header |
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| Images | emd_54130.png | 123 KB | ||
| Map data | emd_54130.map.gz | 750.8 MB | EMDB map data format | |
| Filedesc metadata | emd-54130.cif.gz | 13.5 KB | ||
| Others | emd_54130_additional_1.map.gz emd_54130_half_map_1.map.gz emd_54130_half_map_2.map.gz | 27.6 MB 666.6 MB 666.6 MB | ||
| Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-54130 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-54130 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 9rotM M: atomic model generated by this map |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Map
-Supplemental data
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Sample components
+Entire : Maturation state of mitochondria ribosomal 28S subunit.
+Supramolecule #1: Maturation state of mitochondria ribosomal 28S subunit.
+Macromolecule #1: 28S ribosomal protein S34, mitochondrial
+Macromolecule #2: 28S ribosomal protein S35, mitochondrial
+Macromolecule #3: Aurora kinase A-interacting protein
+Macromolecule #4: 28S ribosomal protein S2, mitochondrial
+Macromolecule #5: 28S ribosomal protein S24, mitochondrial
+Macromolecule #6: 28S ribosomal protein S6, mitochondrial
+Macromolecule #7: 28S ribosomal protein S7, mitochondrial
+Macromolecule #8: 28S ribosomal protein S10, mitochondrial
+Macromolecule #9: 28S ribosomal protein S11, mitochondrial
+Macromolecule #10: 28S ribosomal protein S14, mitochondrial
+Macromolecule #11: 28S ribosomal protein S15, mitochondrial
+Macromolecule #12: 28S ribosomal protein S16, mitochondrial
+Macromolecule #13: 28S ribosomal protein S17, mitochondrial
+Macromolecule #14: 28S ribosomal protein S18b, mitochondrial
+Macromolecule #15: 28S ribosomal protein S18c, mitochondrial
+Macromolecule #16: Small ribosomal subunit protein bS21m
+Macromolecule #17: 28S ribosomal protein S22, mitochondrial
+Macromolecule #18: 28S ribosomal protein S23, mitochondrial
+Macromolecule #19: 28S ribosomal protein S25, mitochondrial
+Macromolecule #20: 28S ribosomal protein S26, mitochondrial
+Macromolecule #21: 28S ribosomal protein S28, mitochondrial
+Macromolecule #22: 28S ribosomal protein S29, mitochondrial
+Macromolecule #23: 28S ribosomal protein S31, mitochondrial
+Macromolecule #24: 28S ribosomal protein S33, mitochondrial
+Macromolecule #25: 28S ribosomal protein S5, mitochondrial
+Macromolecule #26: 28S ribosomal protein S9, mitochondrial
+Macromolecule #27: 28S ribosomal protein S12, mitochondrial
+Macromolecule #28: 28S ribosomal protein S27, mitochondrial
+Macromolecule #29: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
+Macromolecule #30: 12S rRNA N4-methylcytidine (m4C) methyltransferase
+Macromolecule #32: Translation initiation factor IF-2, mitochondrial
+Macromolecule #33: Putative ribosome-binding factor A, mitochondrial
+Macromolecule #31: 12S mitochondrial rRNA
+Macromolecule #34: ZINC ION
+Macromolecule #35: FE2/S2 (INORGANIC) CLUSTER
+Macromolecule #36: ADENOSINE-5'-TRIPHOSPHATE
+Macromolecule #37: GUANOSINE-5'-DIPHOSPHATE
+Macromolecule #38: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
+Macromolecule #39: SPERMINE
+Macromolecule #40: MAGNESIUM ION
+Macromolecule #41: POTASSIUM ION
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Buffer | pH: 7.5 |
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| Vitrification | Cryogen name: ETHANE |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2 |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.9000000000000001 µm / Nominal defocus min: 0.3 µm |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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About Yorodumi




Keywords
Homo sapiens (human)
Authors
Sweden, 1 items
Citation
Z (Sec.)
Y (Row.)
X (Col.)


























Processing
FIELD EMISSION GUN

