Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Pseudouridine synthase PUS1 and initiation factor mtIF2 are human mitoribosomal small subunit assembly factors.
Journal, issue, pages	Nat Commun, Vol. 17, Year 2026
Publish date	Apr 21, 2026
Authors	Vivek Singh / Dmitrii Shiriaev / Lorina Bilalli / Anas Khawaja / Joanna Rorbach /
PubMed Abstract	Assembly of the mitochondrial ribosome (mitoribosome) is a crucial step in mitochondrial gene expression. This process facilitates mitochondrial translation, which produces essential subunits of the ...Assembly of the mitochondrial ribosome (mitoribosome) is a crucial step in mitochondrial gene expression. This process facilitates mitochondrial translation, which produces essential subunits of the oxidative phosphorylation machinery-the cell's primary energy-producing machinery. Disruptions in mitoribosome assembly can lead to severe human diseases. Given its fundamental importance, detailed structural analysis of mitoribosome assembly pathways is essential for advancing our understanding of mitochondrial function in both health and disease. In this study, we characterize twelve distinct assembly states of the mitoribosomal small subunit (mtSSU) isolated from human cells. Our findings reveal the intricate details of the final maturation stages of the mtSSU platform, decoding center, and the 3'-end of 12S rRNA. This process is governed by coordinated actions of assembly factors that ensure precise, stepwise rRNA folding and the integration of mitoribosomal proteins into the developing subunit. Our approach identifies pseudouridine synthase PUS1 and initiation factor mtIF2 as assembly factors, expanding their known roles beyond mt-tRNA maturation and translation, respectively. In addition, the identified assembly intermediates provide insight into the modular nature of mtSSU biogenesis in mitochondria and further link late-stage assembly to the acquisition of translational competence.
External links	Nat Commun / PubMed:42342677 / PubMed Central
Methods	EM (single particle)
Resolution	3.0 - 4.6 Å
Structure data	51873 9h51 EMDB-51873, PDB-9h51: Assembly intermediate of human mitochondrial ribosome small subunit in complex with PUS1, METTL15 and RBFA(IN conformation) (state M1) Method: EM (single particle) / Resolution: 3.1 Å 51874 EMDB Unreleased entry 9h52 EMDB-51874, PDB-9h52: Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1, ERAL1, METTL17, MCAT and TFB1M (state N1) Method: EM (single particle) / Resolution: 3.9 Å 51876 9h54 EMDB-51876, PDB-9h54: Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and partial RBFA (state N2) Method: EM (single particle) / Resolution: 3.0 Å 51877 9h55 EMDB-51877, PDB-9h55: Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and TFB1M (state N3) Method: EM (single particle) / Resolution: 3.4 Å 54129 9ror EMDB-54129, PDB-9ror: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and RBFA (Outward conformation) (State M3) Method: EM (single particle) / Resolution: 3.64 Å 54130 9rot EMDB-54130, PDB-9rot: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15, RBFA, and mtIF2 (State M2.1) Method: EM (single particle) / Resolution: 4.3 Å 54131 9rov EMDB-54131, PDB-9rov: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and RBFA (Inward conformation) (State M2) Method: EM (single particle) / Resolution: 3.1 Å 54163 9rpf EMDB-54163, PDB-9rpf: Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and mS37 (State M4) Method: EM (single particle) / Resolution: 4.6 Å EMDB-57663: human mitochondrial ribosome small subunit: Initiation complex with IF3 (State I2) Method: EM (single particle) / Resolution: 3.3 Å EMDB-57667: Human mitochondrial small ribosomal subunit: Initiation complex with IF2 and IF3 (State I3) Method: EM (single particle) / Resolution: 3.1 Å EMDB-57668: Human mitochondrial small ribosomal subunit: Initiation complex with IF2 and tRNA (state I4) Method: EM (single particle) / Resolution: 3.0 Å EMDB-57669: Human mitochondrial small ribosomal subunit: Assembly intermediate with IF3 and RBFA-out (state I1) Method: EM (single particle) / Resolution: 4.3 Å
Chemicals	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM ChemComp-SPM: SPERMINE ChemComp-MG: Unknown entry ChemComp-K: Unknown entry ChemComp-ZN: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-HOH: WATER ChemComp-SF4*: IRON/SULFUR CLUSTER
Source	homo sapiens (human)
Keywords	RIBOSOME / mitochondria / small subunit / assembly / NOA1 / mitochondrial gene expression / mitoribosome / mitoribosome assembly factors / cryoEM