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- EMDB-51874: Assembly intermediate of human mitochondrial ribosome small subun... -

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Basic information

Entry
Database: EMDB / ID: EMD-51874
TitleAssembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1, ERAL1, METTL17, MCAT and TFB1M (state N1)
Map data
Sample
  • Complex: mitoribosomal small subunit assembly intermediate
    • Protein or peptide: x 29 types
    • RNA: x 1 types
  • Ligand: x 6 types
Keywordsmitochondria / small subunit / assembly / NOA1 / RIBOSOME
Function / homology
Function and homology information


mitochondrial ribosome binding / rRNA modification in the mitochondrion / mitochondrial small ribosomal subunit assembly / mitochondrial transcription factor activity / transcription initiation at mitochondrial promoter / Mitochondrial Fatty Acid Beta-Oxidation / rRNA modification / rRNA (adenine-N6,N6-)-dimethyltransferase activity / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity ...mitochondrial ribosome binding / rRNA modification in the mitochondrion / mitochondrial small ribosomal subunit assembly / mitochondrial transcription factor activity / transcription initiation at mitochondrial promoter / Mitochondrial Fatty Acid Beta-Oxidation / rRNA modification / rRNA (adenine-N6,N6-)-dimethyltransferase activity / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity / RNA folding chaperone / mitochondrial ribosome assembly / Mitochondrial translation elongation / Mitochondrial translation initiation / Mitochondrial ribosome-associated quality control / S-adenosyl-L-methionine binding / Mitochondrial translation termination / rRNA methylation / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial translation / fatty acid beta-oxidation / apoptotic mitochondrial changes / mitochondrial nucleoid / ribosomal small subunit binding / sperm head-tail coupling apparatus / Mitochondrial protein degradation / Transferases; Transferring one-carbon groups; Methyltransferases / mitochondrion organization / apoptotic signaling pathway / Transcriptional activation of mitochondrial biogenesis / fatty acid biosynthetic process / cell junction / regulation of translation / ribosomal small subunit assembly / 4 iron, 4 sulfur cluster binding / ribosomal small subunit biogenesis / small ribosomal subunit / small ribosomal subunit rRNA binding / nuclear membrane / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / tRNA binding / cell population proliferation / mitochondrial inner membrane / rRNA binding / structural constituent of ribosome / ribosome / translation / mitochondrial matrix / protein domain specific binding / mRNA binding / GTPase activity / apoptotic process / GTP binding / nucleolus / mitochondrion / DNA binding / RNA binding / nucleoplasm / metal ion binding / plasma membrane / cytoplasm / cytosol
Similarity search - Function
: / : / : / YqeH-like, C-terminal / GTP-binding protein Era / Era-type guanine nucleotide-binding (G) domain / Era-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein Rsm22-like / : / Mitochondrial small ribosomal subunit Rsm22 ...: / : / : / YqeH-like, C-terminal / GTP-binding protein Era / Era-type guanine nucleotide-binding (G) domain / Era-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein Rsm22-like / : / Mitochondrial small ribosomal subunit Rsm22 / rRNA adenine dimethylase-like, C-terminal / Ribosomal RNA adenine dimethylase / Ribosomal RNA adenine methylase transferase, conserved site / Ribosomal RNA adenine dimethylases signature. / Ribosomal RNA adenine methylase transferase, N-terminal / Ribosomal RNA adenine dimethylases / Ribosomal RNA adenine methyltransferase KsgA/Erm / Ribosomal RNA adenine dimethylase / rRNA adenine N(6)-methyltransferase family profile. / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / 28S ribosomal protein S27, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S22, mitochondrial / : / Mitochondrial 28S ribosomal protein S22 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / 28S ribosomal protein S18b, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / 28S ribosomal protein S24, mitochondrial / Mitochondrial 28S ribosomal protein S34 / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / : / : / Mitochondrial ribosome subunit S24 / Mitochondrial 28S ribosomal protein S34 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / : / Ribosomal protein S24/S35, mitochondrial / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Pentatricopeptide repeat / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Malonyl-CoA ACP transacylase, ACP-binding / 50S ribosome-binding GTPase / Acyl transferase / Acyl transferase domain / Acyl transferase domain in polyketide synthase (PKS) enzymes. / Acyl transferase domain superfamily / GTP binding domain / Acyl transferase/acyl hydrolase/lysophospholipase / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Small GTP-binding protein domain / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / K homology domain superfamily, prokaryotic type / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S5 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal
Similarity search - Domain/homology
MRPS21 isoform 1 / Small ribosomal subunit protein uS12m / GTPase Era, mitochondrial / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein mS34 ...MRPS21 isoform 1 / Small ribosomal subunit protein uS12m / GTPase Era, mitochondrial / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein mS34 / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein uS9m / Malonyl-CoA-acyl carrier protein transacylase, mitochondrial / Nitric oxide-associated protein 1 / Dimethyladenosine transferase 1, mitochondrial / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS31 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein mS26 / Ribosome assembly protein METTL17, mitochondrial / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein mS40
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsSingh V / Shiriaev D / Khawaja A / Rorbach J
Funding support Sweden, 2 items
OrganizationGrant numberCountry
Knut and Alice Wallenberg FoundationKAW2019.0109 Sweden
Swedish Research CouncilVR2016-02179 Sweden
CitationJournal: Nat Commun / Year: 2026
Title: Pseudouridine synthase PUS1 and initiation factor mtIF2 are human mitoribosomal small subunit assembly factors.
Authors: Vivek Singh / Dmitrii Shiriaev / Lorina Bilalli / Anas Khawaja / Joanna Rorbach /
Abstract: Assembly of the mitochondrial ribosome (mitoribosome) is a crucial step in mitochondrial gene expression. This process facilitates mitochondrial translation, which produces essential subunits of the ...Assembly of the mitochondrial ribosome (mitoribosome) is a crucial step in mitochondrial gene expression. This process facilitates mitochondrial translation, which produces essential subunits of the oxidative phosphorylation machinery-the cell's primary energy-producing machinery. Disruptions in mitoribosome assembly can lead to severe human diseases. Given its fundamental importance, detailed structural analysis of mitoribosome assembly pathways is essential for advancing our understanding of mitochondrial function in both health and disease. In this study, we characterize twelve distinct assembly states of the mitoribosomal small subunit (mtSSU) isolated from human cells. Our findings reveal the intricate details of the final maturation stages of the mtSSU platform, decoding center, and the 3'-end of 12S rRNA. This process is governed by coordinated actions of assembly factors that ensure precise, stepwise rRNA folding and the integration of mitoribosomal proteins into the developing subunit. Our approach identifies pseudouridine synthase PUS1 and initiation factor mtIF2 as assembly factors, expanding their known roles beyond mt-tRNA maturation and translation, respectively. In addition, the identified assembly intermediates provide insight into the modular nature of mtSSU biogenesis in mitochondria and further link late-stage assembly to the acquisition of translational competence.
History
DepositionOct 22, 2024-
Header (metadata) releaseJul 8, 2026-
Map releaseJul 8, 2026-
UpdateJul 8, 2026-
Current statusJul 8, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_51874.png

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Map

FileReleased
Projections & slices

Image control

Size
Brightness
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AxesZ (Sec.)Y (Row.)X (Col.)
1.02 Å/pix.
x 600 pix.
= 609.12 Å		1.02 Å/pix.
x 600 pix.
= 609.12 Å		1.02 Å/pix.
x 600 pix.
= 609.12 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0152 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.12
Minimum - Maximum-1.2707397 - 2.7360048
Average (Standard dev.)0.0018124649 (±0.029301036)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 609.12 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_51874_additional_1.map
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Additional map: N1 composite unsharpened

Fileemd_51874_additional_2.map
AnnotationN1 composite unsharpened
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Half map: #2

Fileemd_51874_half_map_1.map
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Half map: #1

Fileemd_51874_half_map_2.map
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Sample components

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Entire : mitoribosomal small subunit assembly intermediate

EntireName: mitoribosomal small subunit assembly intermediate
Components
  • Complex: mitoribosomal small subunit assembly intermediate
    • Protein or peptide: Small ribosomal subunit protein mS34
    • Protein or peptide: Nitric oxide-associated protein 1
    • Protein or peptide: 28S ribosomal protein S5, mitochondrial
    • Protein or peptide: 28S ribosomal protein S6, mitochondrial
    • Protein or peptide: 28S ribosomal protein S12, mitochondrial
    • Protein or peptide: 28S ribosomal protein S15, mitochondrial
    • Protein or peptide: 28S ribosomal protein S16, mitochondrial
    • Protein or peptide: 28S ribosomal protein S17, mitochondrial
    • Protein or peptide: 28S ribosomal protein S18b, mitochondrial
    • Protein or peptide: 28S ribosomal protein S18c, mitochondrial
    • Protein or peptide: MRPS21 isoform 1
    • Protein or peptide: 28S ribosomal protein S22, mitochondrial
    • Protein or peptide: 28S ribosomal protein S23, mitochondrial
    • Protein or peptide: 28S ribosomal protein S25, mitochondrial
    • Protein or peptide: 28S ribosomal protein S26, mitochondrial
    • Protein or peptide: GTPase Era, mitochondrial
    • RNA: 12S mitochondrial rRNA
    • Protein or peptide: 28S ribosomal protein S2, mitochondrial
    • Protein or peptide: 28S ribosomal protein S28, mitochondrial
    • Protein or peptide: 28S ribosomal protein S24, mitochondrial
    • Protein or peptide: 28S ribosomal protein S7, mitochondrial
    • Protein or peptide: 28S ribosomal protein S9, mitochondrial
    • Protein or peptide: 28S ribosomal protein S29, mitochondrial
    • Protein or peptide: 28S ribosomal protein S31, mitochondrial
    • Protein or peptide: 28S ribosomal protein S35, mitochondrial
    • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
    • Protein or peptide: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
    • Protein or peptide: Isoform 2 of Ribosome assembly protein METTL17, mitochondrial
    • Protein or peptide: Dimethyladenosine transferase 1, mitochondrial
    • Protein or peptide: 28S ribosomal protein S27, mitochondrial
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: ZINC ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: IRON/SULFUR CLUSTER

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Supramolecule #1: mitoribosomal small subunit assembly intermediate

SupramoleculeName: mitoribosomal small subunit assembly intermediate / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #2-#16, #1, #17-#30
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: GTPase Era, mitochondrial

MacromoleculeName: GTPase Era, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 48.413941 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAPSWRGAR LVQSVLRVWQ VGPHVARERV IPFSSLLGFQ RRCVSCVAGS AFSGPRLASA SRSNGQGSAL DHFLGFSQPD SSVTPCVPA VSMNRDEQDV LLVHHPDMPE NSRVLRVVLL GAPNAGKSTL SNQLLGRKVF PVSRKVHTTR CQALGVITEK E TQVILLDT ...String:
MAAPSWRGAR LVQSVLRVWQ VGPHVARERV IPFSSLLGFQ RRCVSCVAGS AFSGPRLASA SRSNGQGSAL DHFLGFSQPD SSVTPCVPA VSMNRDEQDV LLVHHPDMPE NSRVLRVVLL GAPNAGKSTL SNQLLGRKVF PVSRKVHTTR CQALGVITEK E TQVILLDT PGIISPGKQK RHHLELSLLE DPWKSMESAD LVVVLVDVSD KWTRNQLSPQ LLRCLTKYSQ IPSVLVMNKV DC LKQKSVL LELTAALTEG VVNGKKLKMR QAFHSHPGTH CPSPAVKDPN TQSVGNPQRI GWPHFKEIFM LSALSQEDVK TLK QYLLTQ AQPGPWEYHS AVLTSQTPEE ICANIIREKL LEHLPQEVPY NVQQKTAVWE EGPGGELVIQ QKLLVPKESY VKLL IGPKG HVISQIAQEA GHDLMDIFLC DVDIRLSVKL LK

UniProtKB: GTPase Era, mitochondrial

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Macromolecule #2: Small ribosomal subunit protein mS34

MacromoleculeName: Small ribosomal subunit protein mS34 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.336084 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: KKVRPRLIAE LARRVRALRE QLNRPRDSQL YAVDYETLTR PFSGRRLPVR AWADVRRESR LLQLLGRLPL FGLGRLVTRK SWLWQHDEP CYWRLTRVRP DYTAQNLDHG KAWGILTFKG KTESEAREIE HVMYHDWRLV PKHEEEAFTA FTPAPEDSLA S VPYPPLLR ...String:
KKVRPRLIAE LARRVRALRE QLNRPRDSQL YAVDYETLTR PFSGRRLPVR AWADVRRESR LLQLLGRLPL FGLGRLVTRK SWLWQHDEP CYWRLTRVRP DYTAQNLDHG KAWGILTFKG KTESEAREIE HVMYHDWRLV PKHEEEAFTA FTPAPEDSLA S VPYPPLLR AMIIAERQKN GDTSTEEPML NVQRIRMEPW DYPAKQEDKG RAKGTPV

UniProtKB: Small ribosomal subunit protein mS34

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Macromolecule #3: Nitric oxide-associated protein 1

MacromoleculeName: Nitric oxide-associated protein 1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 78.565758 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MLPARLPFRL LSLFLRGSAP TAARHGLREP LLERRCAAAS SFQHSSSLGR ELPYDPVDTE GFGEGGDMQE RFLFPEYILD PEPQPTREK QLQELQQQQE EEERQRQQRR EERRQQNLRA RSREHPVVGH PDPALPPSGV NCSGCGAELH CQDAGVPGYL P REKFLRTA ...String:
MLPARLPFRL LSLFLRGSAP TAARHGLREP LLERRCAAAS SFQHSSSLGR ELPYDPVDTE GFGEGGDMQE RFLFPEYILD PEPQPTREK QLQELQQQQE EEERQRQQRR EERRQQNLRA RSREHPVVGH PDPALPPSGV NCSGCGAELH CQDAGVPGYL P REKFLRTA EADGGLARTV CQRCWLLSHH RRALRLQVSR EQYLELVSAA LRRPGPSLVL YMVDLLDLPD ALLPDLPALV GP KQLIVLG NKVDLLPQDA PGYRQRLRER LWEDCARAGL LLAPGHQGPQ RPVKDEPQDG ENPNPPNWSR TVVRDVRLIS AKT GYGVEE LISALQRSWR YRGDVYLVGA TNAGKSTLFN TLLESDYCTA KGSEAIDRAT ISPWPGTTLN LLKFPICNPT PYRM FKRHQ RLKKDSTQAE EDLSEQEQNQ LNVLKKHGYV VGRVGRTFLY SEEQKDNIPF EFDADSLAFD MENDPVMGTH KSTKQ VELT AQDVKDAHWF YDTPGITKEN CILNLLTEKE VNIVLPTQSI VPRTFVLKPG MVLFLGAIGR IDFLQGNQSA WFTVVA SNI LPVHITSLDR ADALYQKHAG HTLLQIPMGG KERMAGFPPL VAEDIMLKEG LGASEAVADI KFSSAGWVSV TPNFKDR LH LRGYTPEGTV LTVRPPLLPY IVNIKGQRIK KSVAYKTKKP PSLMYNVRKK KGKINV

UniProtKB: Nitric oxide-associated protein 1

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Macromolecule #4: 28S ribosomal protein S5, mitochondrial

MacromoleculeName: 28S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 48.094594 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV ...String:
MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV EADMIQQREE WDRKKKMKVK RERGWSGNSW GGISLGPPDP GPCGETYEDF DTRILEVRNV FTMTAKEGRK KS IRVLVAV GNGKGAAGFS IGKATDRMDA FRKAKNRAVH HLHYIERYED HTIFHDISLR FKRTHIKMKK QPKGYGLRCH RAI ITICRL IGIKDMYAKV SGSINMLSLT QGLFRGLSRQ ETHQQLADKK GLHVVEIREE CGPLPIVVAS PRGPLRKDPE PEDE VPDVK LDWEDVKTAQ GMKRSVWSNL KRAAT

UniProtKB: Small ribosomal subunit protein uS5m

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Macromolecule #5: 28S ribosomal protein S6, mitochondrial

MacromoleculeName: 28S ribosomal protein S6, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.250588 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MPRYELALIL KAMQRPETAA TLKRTIEALM DRGAIVRDLE NLGERALPYR ISAHSQQHNR GGYFLVDFYA PTAAVESMVE HLSRDIDVI RGNIVKHPLT QELKECEGIV PVPLAEKLYS TKKRKK

UniProtKB: Small ribosomal subunit protein bS6m

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Macromolecule #6: 28S ribosomal protein S12, mitochondrial

MacromoleculeName: 28S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.200947 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MSWSGLLHGL NTSLTCGPAL VPRLWATCSM ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG VVLCTFTRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HTLQEHQIVL VEGGRTQDLP GVKLTVVRGK YDCGHVQKK

UniProtKB: Small ribosomal subunit protein uS12m

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Macromolecule #7: 28S ribosomal protein S15, mitochondrial

MacromoleculeName: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.903293 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK ...String:
MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK NLRNTNYDVF EKICWGLGIE YTFPPLYYRR AHRRFVTKKA LCIRVFQETQ KLKKRRRALK AAAAAQKQAK RR NPDSPAK AIPKTLKDSQ

UniProtKB: Small ribosomal subunit protein uS15m

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Macromolecule #8: 28S ribosomal protein S16, mitochondrial

MacromoleculeName: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.371899 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MVHLTTLLCK AYRGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNL DRIRHWIGCG AHLSKPMEK LLGLAGFFPL HPMMITNAER LRRKRAREVL LASQKTDAEA TDTEATET

UniProtKB: Small ribosomal subunit protein bS16m

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Macromolecule #9: 28S ribosomal protein S17, mitochondrial

MacromoleculeName: 28S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.526078 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MSVVRSSVHA RWIVGKVIGT KMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLRALP VPRAKHVKHE LAEIVFKVG KVIDPVTGKP CAGTTYLESP LSSETTQLSK NLEELNISSA Q

UniProtKB: Small ribosomal subunit protein uS17m

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Macromolecule #10: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.440629 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP ...String:
MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP QVEPRDLDFS TSHGAVSATP PAPTLVSGDP WYPWYNWKQP PERELSRLRR LYQGHLQEES GPPPESMPKM PP RTPAEAS STGQTGPQSA L

UniProtKB: Small ribosomal subunit protein mS40

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Macromolecule #11: 28S ribosomal protein S18c, mitochondrial

MacromoleculeName: 28S ribosomal protein S18c, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.876786 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MAAVVAVCGG LGRKKLTHLV TAAVSLTHPG THTVLWRRGC SQQVSSNEDL PISMENPYKE PLKKCILCGK HVDYKNVQLL SQFVSPFTG CIYGRHITGL CGKKQKEITK AIKRAQIMGF MPVTYKDPAY LKDPKVCNIR YRE

UniProtKB: Small ribosomal subunit protein bS18m

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Macromolecule #12: MRPS21 isoform 1

MacromoleculeName: MRPS21 isoform 1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 10.659494 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
(ACE)AKHLKFIAR TVMVQEGNVE SAYRTLNRIL TMDGLIEDIK HRRYYEKPCR RRQRESYERC RRIYNMEMAR KINFLM RKN RADPWQGC

UniProtKB: MRPS21 isoform 1

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Macromolecule #13: 28S ribosomal protein S22, mitochondrial

MacromoleculeName: 28S ribosomal protein S22, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 41.337316 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ...String:
MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ERFIVVREPS GTLRKASWEE RDRMIQVYFP KEGRKILTPI IFKEENLRTM YSQDRHVDVL NLCFAQFEPD ST EYIKVHH KTYEDIDKRG KYDLLRSTRY FGGMVWYFVN NKKIDGLLID QIQRDLIDDA TNLVQLYHVL HPDGQSAQGA KDQ AAEGIN LIKVFAKTEA QKGAYIELTL QTYQEALSRH SAAS

UniProtKB: Small ribosomal subunit protein mS22

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Macromolecule #14: 28S ribosomal protein S23, mitochondrial

MacromoleculeName: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.805664 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MAGSRLETVG SIFSRTRDLV RAGVLKEKPL WFDVYDAFPP LREPVFQRPR VRYGKAKAPI QDIWYHEDRI RAKFYSVYGS GQRAFDLFN PNFKSTCQRF VEKYTELQKL GETDEEKLFV ETGKALLAEG VILRRVGEAR TQHGGSHVSR KSEHLSVRPQ T ALEENETQ KEVPQDQHLE APADQSKGLL PP

UniProtKB: Small ribosomal subunit protein mS23

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Macromolecule #15: 28S ribosomal protein S25, mitochondrial

MacromoleculeName: 28S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.146428 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MPMKGRFPIR RTLQYLSQGN VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQIMMFKNM TPSPFLRFYL DSGEQVLVD VETKSNKEIM EHIRKILGKN EETLREEEEE KKQLSHPANF GPRKYCLREC ICEVEGQVPC PSLVPLPKEM R GKYKAALK ADAQD

UniProtKB: Small ribosomal subunit protein mS25

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Macromolecule #16: 28S ribosomal protein S26, mitochondrial

MacromoleculeName: 28S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.25982 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI ...String:
MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI TRENLEARVE AALDSRKNYN WAITREGLVV RPQRRDS

UniProtKB: Small ribosomal subunit protein mS26

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Macromolecule #18: 28S ribosomal protein S2, mitochondrial

MacromoleculeName: 28S ribosomal protein S2, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 33.298238 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT ...String:
MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT RYFRGGMLTN ARLLFGPTVR LPDLIIFLHT LNNIFEPHVA VRDAAKMNIP TVGIVDTNCN PCLITYPVPG ND DSPLAVH LYCRLFQTAI TRAKEKRQQV EALYRLQGQK EPGDQGPAHP PGADMSHSL

UniProtKB: Small ribosomal subunit protein uS2m

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Macromolecule #19: 28S ribosomal protein S28, mitochondrial

MacromoleculeName: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.878846 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MAALCRTRAV AAESHFLRVF LFFRPFRGVG TESGSESGSS NAKEPKTRAG GFASALERHS ELLQKVEPLQ KGSPKNVESF ASMLRHSPL TQMGPAKDKL VIGRIFHIVE NDLYIDFGGK FHCVCRRPEV DGEKYQKGTR VRLRLLDLEL TSRFLGATTD T TVLEANAV LLGIQESKDS RSKEEHHEK

UniProtKB: Small ribosomal subunit protein bS1m

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Macromolecule #20: 28S ribosomal protein S24, mitochondrial

MacromoleculeName: 28S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 19.046164 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MAASVCSGLL GPRVLSWSRE LPCAWRALHT SPVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMWGT FPGCLADQLV LKRRGNQLEI CAVVLRQLSP HKYYFLVGYS ETLLSYFYKC PVRLHLQTVP S KVVYKYL

UniProtKB: Small ribosomal subunit protein uS3m

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Macromolecule #21: 28S ribosomal protein S7, mitochondrial

MacromoleculeName: 28S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.186154 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY ...String:
MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY QVPVPLPDRR RRFLAMKWMI TECRDKKHQR TLMPEKLSHK LLEAFHNQGP VIKRKHDLHK MAEANRALAH YR WW

UniProtKB: Small ribosomal subunit protein uS7m

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Macromolecule #22: 28S ribosomal protein S9, mitochondrial

MacromoleculeName: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.909859 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM ...String:
MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM LLNLEKHQSH LQAKSLLPEK TVTRDVIGSR WLIKEELEEM LVEKLSDLDY MQFIRLLEKL LTSQCGAAEE EF VQRFRRS VTLESKKQLI EPVQYDEQGM AFSKSEGKRK TAKAEAIVYK HGSGRIKVNG IDYQLYFPIT QDREQLMFPF HFV DRLGKH DVTCTVSGGG RSAQAGAIRL AMAKALCSFV TEDEVEWMRQ AGLLTTDPRV RERKKPGQEG ARRKFTWKKR

UniProtKB: Small ribosomal subunit protein uS9m

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Macromolecule #23: 28S ribosomal protein S29, mitochondrial

MacromoleculeName: 28S ribosomal protein S29, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.634406 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL ...String:
MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL LQSSYNKQRF DQPLEASTWL KNFKTTNERF LNQIKVQEKY VWNKRESTEK GSPLGEVVEQ GITRVRNATD AV GIVLKEL KRQSSLGMFH LLVAVDGINA LWGRTTLKRE DKSPIAPEEL ALVHNLRKMM KNDWHGGAIV SALSQTGSLF KPR KAYLPQ ELLGKEGFDA LDPFIPILVS NYNPKEFESC IQYYLENNWL QHEKAPTEEG KKELLFLSNA NPSLLERHCA YL

UniProtKB: Small ribosomal subunit protein mS29

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Macromolecule #24: 28S ribosomal protein S31, mitochondrial

MacromoleculeName: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.391473 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF ...String:
MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF DKQTTKSELL SQLQQHEEES RAQRDAKRPK ISFSNIISDM KVARSATARV RSRPELRIQF DEGYDNYPGQ EK TDDLKKR KNIFTGKRLN IFDMMAVTKE APETDTSPSL WDVEFAKQLA TVNEQPLQNG FEELIQWTKE GKLWEFPINN EAG FDDDGS EFHEHIFLEK HLESFPKQGP IRHFMELVTC GLSKNPYLSV KQKVEHIEWF RNYFNEKKDI LKESNIQFN

UniProtKB: Small ribosomal subunit protein mS31

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Macromolecule #25: 28S ribosomal protein S35, mitochondrial

MacromoleculeName: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 36.898328 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA ...String:
MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA RVVVLRVKLS SLNLDDHAKK KLIKLVGERY CKTTDVLTIK TDRCPLRRQN YDYAVYLLTV LYHESWNTEE WE KSKTEAD MEEYIWENSS SERNILETLL QMKAAEKNME INKEELLGTK EIEEYKKSVV SLKNEEENEN SISQYKESVK RLL NVT

UniProtKB: Small ribosomal subunit protein mS35

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Macromolecule #26: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

MacromoleculeName: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 78.648547 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA ...String:
MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GDQEPSTDYH FQQTGQSEAL EEENDETSRR KAGHQFGVTW RA KNNAERI FSLMPEKNEH SYCTMIRGMV KHRAYEQALN LYTELLNNRL HADVYTFNAL IEATVCAINE KFEEKWSKIL ELL RHMVAQ KVKPNLQTFN TILKCLRRFH VFARSPALQV LREMKAIGIE PSLATYHHII RLFDQPGDPL KRSSFIIYDI MNEL MGKRF SPKDPDDDKF FQSAMSICSS LRDLELAYQV HGLLKTGDNW KFIGPDQHRN FYYSKFFDLI CLMEQIDVTL KWYED LIPS AYFPHSQTMI HLLQALDVAN RLEVIPKIWK DSKEYGHTFR SDLREEILML MARDKHPPEL QVAFADCAAD IKSAYE SQP IRQTAQDWPA TSLNCIAILF LRAGRTQEAW KMLGLFRKHN KIPRSELLNE LMDSAKVSNS PSQAIEVVEL ASAFSLP IC EGLTQRVMSD FAINQEQKEA LSNLTALTSD SDTDSSSDSD SDTSEGK

UniProtKB: Small ribosomal subunit protein mS39

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Macromolecule #27: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial

MacromoleculeName: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: [acyl-carrier-protein] S-malonyltransferase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 43.019453 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MSVRVARVAW VRGLGASYRR GASSFPVPPP GAQGVAELLR DATGAEEEAP WAATERRMPG QCSVLLFPGQ GSQVVGMGRG LLNYPRVRE LYAAARRVLG YDLLELSLHG PQETLDRTVH CQPAIFVASL AAVEKLHHLQ PSVIENCVAA AGFSVGEFAA L VFAGAMEF ...String:
MSVRVARVAW VRGLGASYRR GASSFPVPPP GAQGVAELLR DATGAEEEAP WAATERRMPG QCSVLLFPGQ GSQVVGMGRG LLNYPRVRE LYAAARRVLG YDLLELSLHG PQETLDRTVH CQPAIFVASL AAVEKLHHLQ PSVIENCVAA AGFSVGEFAA L VFAGAMEF AEGLYAVKIR AEAMQEASEA VPSGMLSVLG QPQSKFNFAC LEAREHCKSL GIENPVCEVS NYLFPDCRVI SG HQEALRF LQKNSSKFHF RRTRMLPVSG AFHTRLMEPA VEPLTQALKA VDIKKPLVSV YSNVHAHRYR HPGHIHKLLA QQL VSPVKW EQTMHAIYER KKGRGFPQTF EVGPGRQLGA ILKSCNMQAW KSYSAVDVLQ TLEHVDLDPQ EPPR

UniProtKB: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial

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Macromolecule #28: Isoform 2 of Ribosome assembly protein METTL17, mitochondrial

MacromoleculeName: Isoform 2 of Ribosome assembly protein METTL17, mitochondrial
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 50.807547 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAALKCLLT LGRWCPGLGV APQARALAAL VPGVTQVDNK SGFLQKRPHR QHPGILKLPH VRLPQALANG AQLLLLGSAG PTMENQVQT LTSYLWSRHL PVEPEELQRR ARHLEKKFLE NPDLSQTEEK LRGAVLHALR KTTYHWQELS YTEGLSLVYM A ARLDGGFA ...String:
MAAALKCLLT LGRWCPGLGV APQARALAAL VPGVTQVDNK SGFLQKRPHR QHPGILKLPH VRLPQALANG AQLLLLGSAG PTMENQVQT LTSYLWSRHL PVEPEELQRR ARHLEKKFLE NPDLSQTEEK LRGAVLHALR KTTYHWQELS YTEGLSLVYM A ARLDGGFA AVSRAFHEIR ARNPAFQPQT LMDFGSGTGS VTWAAHSIWG QSLREYMCVD RSAAMLVLAE KLLKGGSESG EP YIPGVFF RQFLPVSPKV QFDVVVSAFS LSELPSKADR TEVVQTLWRK TGHFLVLVEN GTKAGHSLLM DARDLVLKGK EKS PLDPRP GFVFAPCPHE LPCPQLTNLA CSFSQAYHPI PFSWNKKPKE EKFSMVILAR GSPEEAHRWP RITQPVLKRP RHVH CHLCC PDGHMQHAVL TARRHGRDLY RCARVSSWGD LLPVLTPSAF PPSTAQDPSE S

UniProtKB: Ribosome assembly protein METTL17, mitochondrial

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Macromolecule #29: Dimethyladenosine transferase 1, mitochondrial

MacromoleculeName: Dimethyladenosine transferase 1, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
EC number: Transferases; Transferring one-carbon groups; Methyltransferases
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 39.600953 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAASGKLSTC RLPPLPTIRE IIKLLRLQAA KQLSQNFLLD LRLTDKIVRK AGNLTNAYVY EVGPGPGGIT RSILNADVAE LLVVEKDTR FIPGLQMLSD AAPGKLRIVH GDVLTFKVEK AFSESLKRPW EDDPPNVHII GNLPFSVSTP LIIKWLENIS C RDGPFVYG ...String:
MAASGKLSTC RLPPLPTIRE IIKLLRLQAA KQLSQNFLLD LRLTDKIVRK AGNLTNAYVY EVGPGPGGIT RSILNADVAE LLVVEKDTR FIPGLQMLSD AAPGKLRIVH GDVLTFKVEK AFSESLKRPW EDDPPNVHII GNLPFSVSTP LIIKWLENIS C RDGPFVYG RTQMTLTFQK EVAERLAANT GSKQRSRLSV MAQYLCNVRH IFTIPGQAFV PKPEVDVGVV HFTPLIQPKI EQ PFKLVEK VVQNVFQFRR KYCHRGLRML FPEAQRLEST GRLLELADID PTLRPRQLSI SHFKSLCDVY RKMCDEDPQL FAY NFREEL KRRKSKNEEK EEDDAENYRL

UniProtKB: Dimethyladenosine transferase 1, mitochondrial

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Macromolecule #30: 28S ribosomal protein S27, mitochondrial

MacromoleculeName: 28S ribosomal protein S27, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 47.669262 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM ...String:
MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM MQEAFEVPST QLLSLYVLFH CLAKKTDFSW EEERNFGASL LLPGLKQKNS VGFSSQLYGY ALLGKVELQQ GL RAVYHNM PLIWKPGYLD RALQVMEKVA ASPEDIKLCR EALDVLGAVL KALTSADGAS EEQSQNDEDN QGSEKLVEQL DIE ETEQSK LPQYLERFKA LHSKLQALGK IESEGLLSLT TQLVKEKLST CEAEDIATYE QNLQQWHLDL VQLIQREQQQ REQA KQEYQ AQKAAKASA

UniProtKB: Small ribosomal subunit protein mS27

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Macromolecule #17: 12S mitochondrial rRNA

MacromoleculeName: 12S mitochondrial rRNA / type: rna / ID: 17 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 306.449312 KDa
SequenceString: AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC ...String:
AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC AGCAGUGAUU AACCUUUAGC AAUAAACGAA AGUUUAACUA AGCUAUACUA ACCCCAGGGU UGGUCAAUUU CG UGCCAGC CACCGCGGUC ACACGAUUAA CCCAAGUCAA UAGAAGCCGG CGUAAAGAGU GUUUUAGAUC ACCCCCUCCC CAA UAAAGC UAAAACUCAC CUGAGUUGUA AAAAACUCCA GUUGACACAA AAUAGACUAC GAAAGUGGCU UUAACAUAUC UGAA CACAC AAUAGCUAAG ACCCAAACUG GGAUUAGAUA CCCCACUAUG CUUAGCCCUA AACCUCAACA GUUAAAUCAA CAAAA CUGC UCGCCAGAAC ACUACGAGCC ACAGCUUAAA ACUCAAAGGA CCUGGCGGUG CUUCAUAUCC CUCUAGAGGA GCCUGU UCU GUAAUCGAUA AACCCCGAUC AACCUCACCA CCUCUUGCUC AGCCUAUAUA CCGCCAUCUU CAGCAAACCC UGAUGAA GG CUACAAAGUA AGCGCAAGUA CCCACGUAAA GACGUUAGGU CAAGGUGUAG CCCAUGAGGU GGCAAGAAAU GGGCUACA U UUUCUACCCC AGAAAACUAC GAUAGCCCUU AUGAAACUUA AGGGUCGAAG GUGGAUUUAG CAGUAAACUG AGAGUAGAG UGCUUAGUUG AACAGGGCCC UGAAGCGCGU ACACACCGCC CGUCACCCUC CUCAAGUAUA CUUCAAAGGA CAUUUAACUA AAACCCCUA CGCAUUUAUA UAGAGGAGAC AAGUCGUAAC AUGGUAAGUG UACUGGAAAG UGCACUUGGA CGAACC

GENBANK: GENBANK: MN849517.1

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Macromolecule #31: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 31 / Number of copies: 2 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Macromolecule #32: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 32 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #33: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 33 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Macromolecule #34: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 34 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #35: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 35 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #36: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 36 / Number of copies: 1 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.4 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

8csp

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.6) / Number images used: 11115
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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