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TitleAssembly of the Xrn2/Rat1-Rai1-Rtt103 termination complexes in mesophilic and thermophilic organisms.
Journal, issue, pagesStructure, Vol. 33, Issue 2, Page 300-310.e6, Year 2025
Publish dateFeb 6, 2025
AuthorsAlzbeta Dikunova / Nikola Noskova / Jan H Overbeck / Martin Polak / David Stelzig / David Zapletal / Karel Kubicek / Jiri Novacek / Remco Sprangers / Richard Stefl /
PubMed AbstractThe 5'-3' exoribonuclease Xrn2, known as Rat1 in yeasts, terminates mRNA transcription by RNA polymerase II (RNAPII). In the torpedo model of termination, the activity of Xrn2/Rat1 is enhanced by ...The 5'-3' exoribonuclease Xrn2, known as Rat1 in yeasts, terminates mRNA transcription by RNA polymerase II (RNAPII). In the torpedo model of termination, the activity of Xrn2/Rat1 is enhanced by Rai1, which is recruited to the termination site by Rtt103, an adaptor protein binding to the RNAPII C-terminal domain (CTD). The overall architecture of the Xrn2/Rat1-Rai1-Rtt103 complex remains unknown. We combined structural biology methods to characterize the torpedo complex from Saccharomyces cerevisiae and Chaetomium thermophilum. Comparison of the structures from these organisms revealed a conserved protein core fold of the subunits, but significant variability in their interaction interfaces. We found that in the mesophile, Rtt103 utilizes an unstructured region to augment a Rai1 β-sheet, while in the thermophile Rtt103 binds to a C-terminal helix of Rai1 via its CTD-interacting domain with an α-helical fold. These different torpedo complex assemblies reflect adaptations to the environment and impact complex recruitment to RNAPII.
External linksStructure / PubMed:39657659
MethodsEM (single particle)
Resolution2.65 - 3.28 Å
Structure data

18199

8q6v

EMDB-18199, PDB-8q6v:
Cryo-EM structure of S. cerevisiae Rai1-Rat1 dimer.
Method: EM (single particle) / Resolution: 3.23 Å

50048

9exs

EMDB-50048, PDB-9exs:
Cryo-EM structure of Ch. thermophilum Rai1-Rat1 dimer.
Method: EM (single particle) / Resolution: 3.28 Å

50566

9fms

EMDB-50566, PDB-9fms:
Cryo-EM structure of S. cerevisiae Rai1-Rat1-Rtt103(252-273) complex.
Method: EM (single particle) / Resolution: 2.65 Å

Chemicals

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • thermochaetoides thermophila (fungus)
KeywordsRNA BINDING PROTEIN / Rai1 nuclease / Rtt103 binding / structured / RNA binding

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