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TitleTowards a molecular picture of the archaeal cell surface.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 10401, Year 2024
Publish dateNov 29, 2024
AuthorsMatthew C Gaines / Michail N Isupov / Mathew McLaren / Clara L Mollat / Risat Ul Haque / Jake K Stephenson / Shamphavi Sivabalasarma / Cyril Hanus / Daniel Kattnig / Vicki A M Gold / Sonja Albers / Bertram Daum /
PubMed AbstractArchaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These ...Archaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These include rotary swimming propellers analogous to bacterial flagella (archaella), pili for twitching motility (Aap), adhesive fibres (threads), and filaments facilitating homologous recombination upon UV stress (UV pili). Here, we use cryo-electron microscopy to describe the structure of the S. acidocaldarius archaellum at 2.0 Å resolution, and update the structures of the thread and the Aap pilus at 2.7 Å and 2.6 Å resolution, respectively. We define features unique to archaella of the order Sulfolobales and compare their structure to those of Aap and threads in the context of the S-layer. We define distinct N-glycan patterns in the three filaments and identify a putative O-glycosylation site in the thread. Finally, we ascertain whether N-glycan truncation leads to structural changes in archaella and Aap.
External linksNat Commun / PubMed:39614099 / PubMed Central
MethodsEM (helical sym.)
Resolution2.03 - 2.65 Å
Structure data

18700

8qx4

EMDB-18700, PDB-8qx4:
Sulfolobus acidocaldarius Archaellum filament.
Method: EM (helical sym.) / Resolution: 2.03 Å

19608

8rzl

EMDB-19608, PDB-8rzl:
Sulfolobus acidocaldarius threads (0406) filament.
Method: EM (helical sym.) / Resolution: 2.65 Å

19960

9ets

EMDB-19960, PDB-9ets:
Sulfolobus acidocaldarius AAP filament
Method: EM (helical sym.) / Resolution: 2.6 Å

19961

9ett

EMDB-19961, PDB-9ett:
Structure of the archaellum of Sulfolobus acidocaldarius strain MW039 (delta agl3 mutant).
Method: EM (helical sym.) / Resolution: 2.37 Å

19990

9ev0

EMDB-19990, PDB-9ev0:
Structure of the AAP filament of Sulfolobus acidocaldarius strain MW039 (delta agl3 mutant).
Method: EM (helical sym.) / Resolution: 2.38 Å

Chemicals

ChemComp-MAN:
alpha-D-mannopyranose

Source
  • sulfolobus acidocaldarius (acidophilic)
KeywordsPROTEIN FIBRIL / cell surface appendage / N-glycosylation / O-glycosylation / mutagenesis

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