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Structure paper

TitleStructural basis for the transport and regulation mechanism of the multidrug resistance-associated protein 2.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 484, Year 2025
Publish dateJan 8, 2025
AuthorsEriko Koide / Harlan L Pietz / Jean Beltran / Jue Chen /
PubMed AbstractMultidrug resistance-associated protein 2 (MRP2) is an ATP-powered exporter important for maintaining liver homeostasis and a potential contributor to chemotherapeutic resistance. Using cryogenic ...Multidrug resistance-associated protein 2 (MRP2) is an ATP-powered exporter important for maintaining liver homeostasis and a potential contributor to chemotherapeutic resistance. Using cryogenic electron microscopy (cryo-EM), we determine the structures of human MRP2 in three conformational states: an autoinhibited state, a substrate-bound pre-translocation state, and an ATP-bound post-translocation state. In the autoinhibited state, the cytosolic regulatory (R) domain plugs into the transmembrane substrate-binding site and extends into the cytosol to form a composite ATP-binding site at the surface of nucleotide-binding domain 2. Substrate displaces the R domain, permitting conformational changes necessary for transport. These observations suggest that the R domain functions as a selectivity gauge, where only at sufficiently high concentrations can the substrate effectively initiate transport. Comparative structural analyzes of MRP2 bound to various substrates, as determined in this study and others, reveal how MRP2 recognizes a diverse array of compounds, supporting its role in multidrug resistance.
External linksNat Commun / PubMed:39779684 / PubMed Central
MethodsEM (single particle)
Resolution2.75 - 3.62 Å
Structure data

44833

9br2

EMDB-44833, PDB-9br2:
Inward-facing, ATP-bound Multidrug Resistance-associated Protein 2 (MRP2) (E1462Q)
Method: EM (single particle) / Resolution: 3.41 Å

44911

9buk

EMDB-44911, PDB-9buk:
Outward-facing, ATP-bound Multidrug Resistance-associated Protein 2 (MRP2) (E1462Q)
Method: EM (single particle) / Resolution: 3.4 Å

45099

9c12

EMDB-45099, PDB-9c12:
Leukotriene C4-bound Multidrug Resistance-associated Protein 2 (MRP2)
Method: EM (single particle) / Resolution: 2.75 Å

45159

9c2i

EMDB-45159, PDB-9c2i:
Inward-facing, ligand-free Multidrug Resistance-associated protein 2 (MRP2)
Method: EM (single particle) / Resolution: 3.62 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry


ChemComp, No image

ChemComp-UNL:
Unknown ligand

ChemComp-CLR:
CHOLESTEROL

ChemComp-LTX:
(5~{S},6~{R},7~{E},9~{E},11~{Z},14~{Z})-6-[(2~{R})-2-[[(4~{S})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-(

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / ABC transporter / Multidrug resistance / Regulatory domain / Autoinhibition / nucleotide binding / Substrate

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