EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM reveals transition states of the Acinetobacter baumannii F-ATPase rotary subunits γ and ε, unveiling novel compound targets.
ジャーナル・号・ページ	FASEB J, Vol. 38, Issue 20, Page e70131, Year 2024
掲載日	2024年10月28日
著者	Khoa Cong Minh Le / Chui Fann Wong / Volker Müller / Gerhard Grüber /
PubMed 要旨	Priority 1: critical WHO pathogen Acinetobacter baumannii depends on ATP synthesis and ATP:ADP homeostasis and its bifunctional FF-ATP synthase. While synthesizing ATP, it regulates ATP cleavage by ...Priority 1: critical WHO pathogen Acinetobacter baumannii depends on ATP synthesis and ATP:ADP homeostasis and its bifunctional FF-ATP synthase. While synthesizing ATP, it regulates ATP cleavage by its inhibitory ε subunit to prevent wasteful ATP consumption. We determined cryo-electron microscopy structures of the ATPase active A. baumannii F-αßγε mutant in four distinct conformational states, revealing four transition states and structural transformation of the ε's C-terminal domain, forming the switch of an ATP hydrolysis off- and an ATP synthesis on-state based. These alterations go in concert with altered motions and interactions in the catalytic- and rotary subunits of this engine. These A. baumannii interacting sites provide novel pathogen-specific targets for inhibitors, with the aim of ATP depletion and/or ATP synthesis and growth inhibition. Furthermore, the presented diversity to other bacterial F-ATP synthases extends the view of structural elements regulating such a catalyst.
リンク	FASEB J / PubMed:39467208 / PubMed Central
手法	EM (単粒子)
解像度	2.89 - 3.18 Å
構造データ	60117 8zi0 EMDB-60117, PDB-8zi0: Cryo-EM reveals transition states of the Acinetobacter baumannii F1-ATPase rotary subunits gamma and epsilon and novel compound targets - Conformation 1 手法: EM (単粒子) / 解像度: 3.18 Å 60118 8zi1 EMDB-60118, PDB-8zi1: Cryo-EM reveals transition states of the Acinetobacter baumannii F1-ATPase rotary subunits gamma and epsilon and novel compound targets - Conformation 2 手法: EM (単粒子) / 解像度: 2.92 Å 60119 8zi2 EMDB-60119, PDB-8zi2: Cryo-EM reveals transition states of the Acinetobacter baumannii F1-ATPase rotary subunits gamma and epsilon and novel compound targets - Conformation 3 手法: EM (単粒子) / 解像度: 2.99 Å 60120 8zi3 EMDB-60120, PDB-8zi3: Cryo-EM reveals transition states of the Acinetobacter baumannii F1-ATPase rotary subunits gamma and epsilon and novel compound targets - Conformation 4 手法: EM (単粒子) / 解像度: 2.89 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト
由来	acinetobacter baumannii ab5075 (バクテリア)
キーワード	HYDROLASE / ATP hydrolysis / F1-ATPase