EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Disulfide stabilization reveals conserved dynamic features between SARS-CoV-1 and SARS-CoV-2 spikes.
ジャーナル・号・ページ	Life Sci Alliance, Vol. 6, Issue 9, Year 2023
掲載日	2023年7月4日
著者	Xixi Zhang / Zimu Li / Yanjun Zhang / Yutong Liu / Jingjing Wang / Banghui Liu / Qiuluan Chen / Qian Wang / Lutang Fu / Peiyi Wang / Xiaolin Zhong / Liang Jin / Qihong Yan / Ling Chen / Jun He / Jincun Zhao / Xiaoli Xiong /
PubMed 要旨	SARS-CoV-2 spike protein (S) is structurally dynamic and has been observed by cryo-EM to adopt a variety of prefusion conformations that can be categorized as locked, closed, and open. S-trimers ...SARS-CoV-2 spike protein (S) is structurally dynamic and has been observed by cryo-EM to adopt a variety of prefusion conformations that can be categorized as locked, closed, and open. S-trimers adopting locked conformations are tightly packed featuring structural elements incompatible with RBD in the "up" position. For SARS-CoV-2 S, it has been shown that the locked conformations are transient under neutral pH. Probably because of their transience, locked conformations remain largely uncharacterized for SARS-CoV-1 S. In this study, we introduced x1, x2, and x3 disulfides into SARS-CoV-1 S. Some of these disulfides have been shown to preserve rare locked conformations when introduced to SARS-CoV-2 S. Introduction of these disulfides allowed us to image a variety of locked and other rare conformations for SARS-CoV-1 S by cryo-EM. We identified bound cofactors and structural features that are associated with SARS-CoV-1 S locked conformations. We compare newly determined structures with other available spike structures of SARS-related CoVs to identify conserved features and discuss their possible functions.
リンク	Life Sci Alliance / PubMed:37402591 / PubMed Central
手法	EM (単粒子)
解像度	2.57 - 4.05 Å
構造データ	34417 8h0x EMDB-34417, PDB-8h0x: Structure of SARS-CoV-1 Spike Protein with Engineered x1 Disulfide (S370C and D967C), Locked-1 Conformation 手法: EM (単粒子) / 解像度: 2.57 Å 34418 8h0y EMDB-34418, PDB-8h0y: Structure of SARS-CoV-1 Spike Protein with Engineered x1 Disulfide (S370C and D967C), Locked-112 Conformation 手法: EM (単粒子) / 解像度: 2.85 Å 34419 8h0z EMDB-34419, PDB-8h0z: Structure of SARS-CoV-1 Spike Protein with Engineered x1 Disulfide (S370C and D967C), Locked-122 Conformation 手法: EM (単粒子) / 解像度: 2.99 Å 34420 8h10 EMDB-34420, PDB-8h10: Structure of SARS-CoV-1 Spike Protein with Engineered x1 Disulfide (S370C and D967C), Locked-2 Conformation 手法: EM (単粒子) / 解像度: 2.99 Å 34421 8h11 EMDB-34421, PDB-8h11: Structure of SARS-CoV-1 Spike Protein with Engineered x1 Disulfide (S370C and D967C), Closed Conformation 手法: EM (単粒子) / 解像度: 2.72 Å 34422 8h12 EMDB-34422, PDB-8h12: Structure of SARS-CoV-1 Spike Protein with Engineered x2 Disulfide (G400C and V969C), Locked-2 Conformation 手法: EM (単粒子) / 解像度: 3.44681 Å 34423 8h13 EMDB-34423, PDB-8h13: Structure of SARS-CoV-1 Spike Protein with Engineered x2 Disulfide (G400C and V969C), Closed Conformation 手法: EM (単粒子) / 解像度: 4.05 Å 34424 8h14 EMDB-34424, PDB-8h14: Structure of SARS-CoV-1 Spike Protein with Engineered x3 Disulfide (D414C and V969C), Locked-1 Conformation 手法: EM (単粒子) / 解像度: 3.39 Å 34425 8h15 EMDB-34425, PDB-8h15: Structure of SARS-CoV-1 Spike Protein (S/native) at pH 5.5, Closed Conformation 手法: EM (単粒子) / 解像度: 3.14182 Å 34426 8h16 EMDB-34426, PDB-8h16: Structure of SARS-CoV-1 Spike Protein (S/native) at pH 5.5, Open Conformation 手法: EM (単粒子) / 解像度: 3.35534 Å
化合物	ChemComp-EIC: LINOLEIC ACID / リノ-ル酸 / リノール酸 ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-BLA: BILIVERDINE IX ALPHA / 19,24-ジヒドロ-1-ヒドロキシ-3,7,13,18-テトラメチル-2,17-ジビニル-19-オキソ-22H-ビリ(以下略)
由来	severe acute respiratory syndrome coronavirus (SARS コロナウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / PROTEIN ENGINEERING (タンパク質工学) / SPIKE PROTEIN (スパイクタンパク質) / SARS-COV-1 (SARSコロナウイルス) / SARS-COV (SARSコロナウイルス)