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TitleStructures of the free and capped ends of the actin filament.
Journal, issue, pagesScience, Vol. 380, Issue 6651, Page 1287-1292, Year 2023
Publish dateJun 23, 2023
AuthorsPeter J Carman / Kyle R Barrie / Grzegorz Rebowski / Roberto Dominguez /
PubMed AbstractThe barbed and pointed ends of the actin filament (F-actin) are the sites of growth and shrinkage and the targets of capping proteins that block subunit exchange, including CapZ at the barbed end and ...The barbed and pointed ends of the actin filament (F-actin) are the sites of growth and shrinkage and the targets of capping proteins that block subunit exchange, including CapZ at the barbed end and tropomodulin at the pointed end. We describe cryo-electron microscopy structures of the free and capped ends of F-actin. Terminal subunits at the free barbed end adopt a "flat" F-actin conformation. CapZ binds with minor changes to the barbed end but with major changes to itself. By contrast, subunits at the free pointed end adopt a "twisted" monomeric actin (G-actin) conformation. Tropomodulin binding forces the second subunit into an F-actin conformation. The structures reveal how the ends differ from the middle in F-actin and how these differences control subunit addition, dissociation, capping, and interactions with end-binding proteins.
External linksScience / PubMed:37228182 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution2.26 - 3.3 Å
Structure data

28932

8f8p

EMDB-28932, PDB-8f8p:
Cryo-EM structure of F-actin in the ADP state
Method: EM (helical sym.) / Resolution: 2.26 Å

28933

8f8q

EMDB-28933, PDB-8f8q:
Cryo-EM structure of the CapZ-capped barbed end of F-actin
Method: EM (single particle) / Resolution: 2.79 Å

28934

8f8r

EMDB-28934, PDB-8f8r:
Cryo-EM structure of the free barbed end of F-actin
Method: EM (single particle) / Resolution: 3.3 Å

28935

8f8s

EMDB-28935, PDB-8f8s:
Cryo-EM structure of the free pointed end of F-actin
Method: EM (single particle) / Resolution: 2.84 Å

28936

8f8t

EMDB-28936, PDB-8f8t:
Cryo-EM structure of the Tropomodulin-capped pointed end of F-actin
Method: EM (single particle) / Resolution: 3.26 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-MG:
Unknown entry

Source
  • oryctolagus cuniculus (rabbit)
  • rabbit (rabbit)
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / Actin cytoskeleton / filament / F-actin / Barbed end capping protein / CapZ / barbed-end / pointed-end / Tmod

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