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TitleStructure of a membrane tethering complex incorporating multiple SNAREs.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 2, Page 246-254, Year 2024
Publish dateJan 9, 2024
AuthorsKevin A DAmico / Abigail E Stanton / Jaden D Shirkey / Sophie M Travis / Philip D Jeffrey / Frederick M Hughson /
PubMed AbstractMost membrane fusion reactions in eukaryotic cells are mediated by multisubunit tethering complexes (MTCs) and SNARE proteins. MTCs are much larger than SNAREs and are thought to mediate the initial ...Most membrane fusion reactions in eukaryotic cells are mediated by multisubunit tethering complexes (MTCs) and SNARE proteins. MTCs are much larger than SNAREs and are thought to mediate the initial attachment of two membranes. Complementary SNAREs then form membrane-bridging complexes whose assembly draws the membranes together for fusion. Here we present a cryo-electron microscopy structure of the simplest known MTC, the 255-kDa Dsl1 complex of Saccharomyces cerevisiae, bound to the two SNAREs that anchor it to the endoplasmic reticulum. N-terminal domains of the SNAREs form an integral part of the structure, stabilizing a Dsl1 complex configuration with unexpected similarities to the 850-kDa exocyst MTC. The structure of the SNARE-anchored Dsl1 complex and its comparison with exocyst reveal what are likely to be common principles underlying MTC function. Our structure also implies that tethers and SNAREs can work together as a single integrated machine.
External linksNat Struct Mol Biol / PubMed:38196032 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution4.5 - 5.73 Å
Structure data

28204

8eki

EMDB-28204, PDB-8eki:
CryoEM structure of the Dsl1 complex bound to SNAREs Sec20 and Use1
Method: EM (single particle) / Resolution: 4.5 Å

PDB-8ftu:
Crystal structure of the SNARE Use1 bound to Dsl1 complex subunits Sec39 and Dsl1, Revised Use1 structure
Method: X-RAY DIFFRACTION / Resolution: 5.73 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
  • kluyveromyces lactis nrrl y-1140 (yeast)
KeywordsTRANSPORT PROTEIN / Tether / SNARE / Complex / membrane trafficking / SNARE protein / COPI / vesicle / multisubunit tethering complex / Dsl1 complex / CATCHR complex

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