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TitleFusion protein strategies for cryo-EM study of G protein-coupled receptors.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4366, Year 2022
Publish dateJul 28, 2022
AuthorsKaihua Zhang / Hao Wu / Nicholas Hoppe / Aashish Manglik / Yifan Cheng /
PubMed AbstractSingle particle cryogenic-electron microscopy (cryo-EM) is used extensively to determine structures of activated G protein-coupled receptors (GPCRs) in complex with G proteins or arrestins. However, ...Single particle cryogenic-electron microscopy (cryo-EM) is used extensively to determine structures of activated G protein-coupled receptors (GPCRs) in complex with G proteins or arrestins. However, applying it to GPCRs without signaling proteins remains challenging because most receptors lack structural features in their soluble domains to facilitate image alignment. In GPCR crystallography, inserting a fusion protein between transmembrane helices 5 and 6 is a highly successful strategy for crystallization. Although a similar strategy has the potential to broadly facilitate cryo-EM structure determination of GPCRs alone without signaling protein, the critical determinants that make this approach successful are not yet clear. Here, we address this shortcoming by exploring different fusion protein designs, which lead to structures of antagonist bound A adenosine receptor at 3.4 Å resolution and unliganded Smoothened at 3.7 Å resolution. The fusion strategies explored here are likely applicable to cryo-EM interrogation of other GPCRs and small integral membrane proteins.
External linksNat Commun / PubMed:35902590 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 6.7 Å
Structure data

25648

7t32

EMDB-25648, PDB-7t32:
CryoEM structure of the adenosine 2A receptor-BRIL/Anti BRIL Fab complex with ZM241385
Method: EM (single particle) / Resolution: 3.4 Å

27062

8cxo

EMDB-27062, PDB-8cxo:
Cryo-EM structure of the unliganded mSMO-PGS2 in a lipidic environment
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-27063: Cryo-EM structure of the unliganded mSMO-PGS1 in a lipidic environment
Method: EM (single particle) / Resolution: 6.7 Å

Chemicals

ChemComp-ZMA:
4-{2-[(7-amino-2-furan-2-yl[1,2,4]triazolo[1,5-a][1,3,5]triazin-5-yl)amino]ethyl}phenol / antagonist*YM

ChemComp-CLR:
CHOLESTEROL

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
  • pyrococcus abyssi (strain ge5 / orsay) (archaea)
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / A2AAR / GPCR / ADENOSINE RECEPTOR / G-protein coupled receptor / Smoothened / unliganded state / lipid system

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