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TitleThe GET insertase exhibits conformational plasticity and induces membrane thinning.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 7355, Year 2023
Publish dateNov 14, 2023
AuthorsMelanie A McDowell / Michael Heimes / Giray Enkavi / Ákos Farkas / Daniel Saar / Klemens Wild / Blanche Schwappach / Ilpo Vattulainen / Irmgard Sinning /
PubMed AbstractThe eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum. In the cytosol, the Get3 chaperone ...The eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum. In the cytosol, the Get3 chaperone captures the TA protein substrate and delivers it to the Get1/Get2 membrane protein complex (GET insertase), which then inserts the substrate via a membrane-embedded hydrophilic groove. Here, we present structures, atomistic simulations and functional data of human and Chaetomium thermophilum Get1/Get2/Get3. The core fold of the GET insertase is conserved throughout eukaryotes, whilst thinning of the lipid bilayer occurs in the vicinity of the hydrophilic groove to presumably lower the energetic barrier of membrane insertion. We show that the gating interaction between Get2 helix α3' and Get3 drives conformational changes in both Get3 and the Get1/Get2 membrane heterotetramer. Thus, we provide a framework to understand the conformational plasticity of the GET insertase and how it remodels its membrane environment to promote substrate insertion.
External linksNat Commun / PubMed:37963916 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.8 - 5.0 Å
Structure data

16801

8cr1

EMDB-16801, PDB-8cr1:
Homo sapiens Get1/Get2 heterotetramer in complex with a Get3 dimer
Method: EM (single particle) / Resolution: 3.2 Å

16802

8cr2

EMDB-16802, PDB-8cr2:
Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in complex with a Get3 dimer
Method: EM (single particle) / Resolution: 4.2 Å

16817

8odu

EMDB-16817, PDB-8odu:
Chaetomium thermophilum Get1/Get2 heterotetramer in complex with a Get3 dimer (amphipol)
Method: EM (single particle) / Resolution: 5.0 Å

16819

8odv

EMDB-16819, PDB-8odv:
Chaetomium thermophilum Get1/Get2 heterotetramer in complex with a Get3 dimer (nanodisc)
Method: EM (single particle) / Resolution: 4.7 Å

PDB-8cqz:
Homo sapiens Get3 in complex with the Get1 cytoplasmic domain
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • Thermochaetoides thermophila (fungus)
  • thermochaetoides thermophila dsm 1495 (fungus)
KeywordsPROTEIN TRANSPORT / membrane protein insertion / GET pathway / tail anchored membrane protein / MEMBRANE PROTEIN

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