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-Structure paper
Title | Structure of the native chemotaxis core signaling unit from phage E-protein lysed cells. |
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Journal, issue, pages | mBio, Vol. 14, Issue 5, Page e0079323, Year 2023 |
Publish date | Sep 29, 2023 |
Authors | C Keith Cassidy / Zhuan Qin / Thomas Frosio / Khoosheh Gosink / Zhengyi Yang / Mark S P Sansom / Phillip J Stansfeld / John S Parkinson / Peijun Zhang / |
PubMed Abstract | Bacterial chemotaxis is a ubiquitous behavior that enables cell movement toward or away from specific chemicals. It serves as an important model for understanding cell sensory signal transduction and ...Bacterial chemotaxis is a ubiquitous behavior that enables cell movement toward or away from specific chemicals. It serves as an important model for understanding cell sensory signal transduction and motility. Characterization of the molecular mechanisms underlying chemotaxis is of fundamental interest and requires a high-resolution structural picture of the sensing machinery, the chemosensory array. In this study, we combine cryo-electron tomography and molecular simulation to present the complete structure of the core signaling unit, the basic building block of chemosensory arrays, from . Our results provide new insight into previously poorly-resolved regions of the complex and offer a structural basis for designing new experiments to test mechanistic hypotheses. |
External links | mBio / PubMed:37772839 / PubMed Central |
Methods | EM (subtomogram averaging) |
Resolution | 11.0 - 12.5 Å |
Structure data | EMDB-15641: Structure of the Native Chemotaxis Core Signalling Complex from E-gene lysed E. coli cells. EMDB-15642: Native Chemotaxis Core Signalling Complex from E. coli, with focused alignment on the baseplate (CheA-CheW) EMDB-15643: Native Chemotaxis Core Signalling Complex from E. coli, Focused alignment on ligand binding domain |
Source |
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Keywords | MEMBRANE PROTEIN / Chemotaxis / Signal Transduction |