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- EMDB-15642: Native Chemotaxis Core Signalling Complex from E. coli, with focu... -

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Basic information

Entry
Database: EMDB / ID: EMD-15642
TitleNative Chemotaxis Core Signalling Complex from E. coli, with focused alignment on the baseplate (CheA-CheW)
Map dataNative Chemotaxis Core Signalling Complex from E. coli, focused alignment on the baseplate (CheA-CheW region)
Sample
  • Complex: Native Chemotaxis Core Signalling Complex
KeywordsChemotaxis Core Signalling Complex from E. coli / Chemotaxis / Signal Transduction / Chemoreceptor / Core signaling complex / MEMBRANE PROTEIN
Biological speciesEscherichia coli (E. coli)
Methodsubtomogram averaging / cryo EM / Resolution: 11.0 Å
AuthorsQin Z / Zhang P
Funding support United Kingdom, United States, European Union, 6 items
OrganizationGrant numberCountry
Wellcome Trust203141/Z/16/Z United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BBSRC: B5R00550 B500.01 United Kingdom
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P50AI150481 United States
Wellcome Trust206422/Z/17/Z United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/S003339/1 United Kingdom
European Research Council (ERC)101021133European Union
CitationJournal: mBio / Year: 2023
Title: Structure of the native chemotaxis core signaling unit from phage E-protein lysed cells.
Authors: C Keith Cassidy / Zhuan Qin / Thomas Frosio / Khoosheh Gosink / Zhengyi Yang / Mark S P Sansom / Phillip J Stansfeld / John S Parkinson / Peijun Zhang /
Abstract: Bacterial chemotaxis is a ubiquitous behavior that enables cell movement toward or away from specific chemicals. It serves as an important model for understanding cell sensory signal transduction and ...Bacterial chemotaxis is a ubiquitous behavior that enables cell movement toward or away from specific chemicals. It serves as an important model for understanding cell sensory signal transduction and motility. Characterization of the molecular mechanisms underlying chemotaxis is of fundamental interest and requires a high-resolution structural picture of the sensing machinery, the chemosensory array. In this study, we combine cryo-electron tomography and molecular simulation to present the complete structure of the core signaling unit, the basic building block of chemosensory arrays, from . Our results provide new insight into previously poorly-resolved regions of the complex and offer a structural basis for designing new experiments to test mechanistic hypotheses.
History
DepositionAug 23, 2022-
Header (metadata) releaseFeb 8, 2023-
Map releaseFeb 8, 2023-
UpdateJul 31, 2024-
Current statusJul 31, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15642.png

Downloads & links

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Map

FileDownload / File: emd_15642.map.gz / Format: CCP4 / Size: 27 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNative Chemotaxis Core Signalling Complex from E. coli, focused alignment on the baseplate (CheA-CheW region)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.75 Å/pix.
x 192 pix.
= 527.04 Å		2.75 Å/pix.
x 192 pix.
= 527.04 Å		2.75 Å/pix.
x 192 pix.
= 527.04 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.745 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-0.3184982 - 0.48737842
Average (Standard dev.)0.000000000051696 (±0.038487125)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-96-96-96
Dimensions192192192
Spacing192192192
CellA=B=C: 527.04 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Native Chemotaxis Core Signalling Complex from E. coli,...

Fileemd_15642_half_map_1.map
AnnotationNative Chemotaxis Core Signalling Complex from E. coli, focused alignment on the baseplate (CheA-CheW region), unmasked half map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Native Chemotaxis Core Signalling Complex from E. coli,...

Fileemd_15642_half_map_2.map
AnnotationNative Chemotaxis Core Signalling Complex from E. coli, focused alignment on the baseplate (CheA-CheW region), unmasked half map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Native Chemotaxis Core Signalling Complex

EntireName: Native Chemotaxis Core Signalling Complex
Components
  • Complex: Native Chemotaxis Core Signalling Complex

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Supramolecule #1: Native Chemotaxis Core Signalling Complex

SupramoleculeName: Native Chemotaxis Core Signalling Complex / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Escherichia coli (E. coli)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Average electron dose: 3.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 3.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 11.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 5100
ExtractionNumber tomograms: 33 / Number images used: 20000
Final angle assignmentType: OTHER / Software - Name: PROTOMO (ver. 0.9)

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