EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure snapshots reveal the mechanism of a bacterial membrane lipoprotein -acyltransferase.
ジャーナル・号・ページ	Sci Adv, Vol. 9, Issue 26, Page eadf5799, Year 2023
掲載日	2023年6月30日
著者	Luke Smithers / Oksana Degtjarik / Dietmar Weichert / Chia-Ying Huang / Coilín Boland / Katherine Bowen / Abraham Oluwole / Corinne Lutomski / Carol V Robinson / Eoin M Scanlan / Meitian Wang / Vincent Olieric / Moran Shalev-Benami / Martin Caffrey /
PubMed 要旨	Bacterial lipoproteins (BLPs) decorate the surface of membranes in the cell envelope. They function in membrane assembly and stability, as enzymes, and in transport. The final enzyme in the BLP ...Bacterial lipoproteins (BLPs) decorate the surface of membranes in the cell envelope. They function in membrane assembly and stability, as enzymes, and in transport. The final enzyme in the BLP synthesis pathway is the apolipoprotein -acyltransferase, Lnt, which is proposed to act by a ping-pong mechanism. Here, we use x-ray crystallography and cryo-electron microscopy to chart the structural changes undergone during the progress of the enzyme through the reaction. We identify a single active site that has evolved to bind, individually and sequentially, substrates that satisfy structural and chemical criteria to position reactive parts next to the catalytic triad for reaction. This study validates the ping-pong mechanism, explains the molecular bases for Lnt's substrate promiscuity, and should facilitate the design of antibiotics with minimal off-target effects.
リンク	Sci Adv / PubMed:37390210 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.395 - 3.13 Å
構造データ	15786 8b0k EMDB-15786, PDB-8b0k: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli (Apo form) 手法: EM (単粒子) / 解像度: 3.0 Å 15787 8b0l EMDB-15787, PDB-8b0l: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with PE 手法: EM (単粒子) / 解像度: 3.13 Å 15788 8b0m EMDB-15788, PDB-8b0m: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with PE (C387S mutant) 手法: EM (単粒子) / 解像度: 3.01 Å 15789 8b0n EMDB-15789, PDB-8b0n: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with Lyso-PE 手法: EM (単粒子) / 解像度: 2.67 Å 15790 8b0o EMDB-15790, PDB-8b0o: Cryo-EM structure apolipoprotein N-acyltransferase Lnt from E.coli in complex with FP3 手法: EM (単粒子) / 解像度: 3.02 Å 15791 8b0p EMDB-15791, PDB-8b0p: Cryo-EM structure of apolipoprotein N-acyltransferase Lnt from E. coli in complex with Pam3 手法: EM (単粒子) / 解像度: 2.86 Å PDB-8aq2: In meso structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa covalently linked with TITC 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å PDB-8aq3: In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with PE 手法: X-RAY DIFFRACTION / 解像度: 2.395 Å PDB-8aq4: In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with TITC and lyso-PE 手法: X-RAY DIFFRACTION / 解像度: 2.62 Å
化合物	ChemComp-OLC: (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / L-グリセロ-ル3-オレア-ト ChemComp-QGT: ~{N}-tridecylmethanethioamide ChemComp-FLC: CITRATE ANION / シトラ-ト / クエン酸 ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-HOH: WATER / 水 ChemComp-6OU: [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate / POPE / リン脂質YM ChemComp-PG5: 1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE / トリグライム / トリグリム ChemComp-PG6: 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE / ペンタグリム / ポリエチレングリコール ChemComp-TRS: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / トリス(ヒドロキシメチル)メチルアンモニウム / pH緩衝剤YM / トリスヒドロキシメチルアミノメタン ChemComp-ACT: ACETATE ION / 酢酸イオン / 酢酸塩 ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-OU9: [(2~{S})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-oxidanyl-propan-2-yl] (~{Z})-octadec-9-enoate ChemComp-OJF: [(2~{R})-3-[(2~{R})-3-[[(2~{R})-1-[[(2~{R})-1-[[(2~{R})-6-[(2-aminophenyl)carbonylamino]-1-azanyl-1-oxidanylidene-hexan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]amino]-2-(hexadecanoylamino)-3-oxidanylidene-propyl]sulfanyl-2-hexadecanoyloxy-propyl] hexadecanoate ChemComp-IG7: [(2~{S})-3-[(2~{S})-3-azanyl-2-(hexadecanoylamino)-3-oxidanylidene-propyl]sulfanyl-2-hexadecanoyloxy-propyl] hexadecanoate
由来	escherichia coli k-12 (大腸菌) synthetic construct (人工物) pseudomonas aeruginosa pao1 (緑膿菌)
キーワード	TRANSFERASE (転移酵素) / Lnt / apolipoprotein N-acyltransferase / Bacterial lipoproteins. / bacterial lipoprotein / cryo-EM (低温電子顕微鏡法)