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Structure paper

TitleMolecular and in vivo studies of a glutamate-class prolyl-endopeptidase for coeliac disease therapy.
Journal, issue, pagesNat Commun, Vol. 13, Page 4446-4446, Year 2022
Publish dateMay 11, 2022 (structure data deposition date)
AuthorsDel Amo-Maestro, L. / Mendes, S.R. / Rodriguez-Banqueri, A. / Garzon-Flores, L. / Girbal, M. / Rodriguez-Lagunas, M.J. / Guevara, T. / Franch, A. / Perez-Cano, F.J. / Eckhard, U. / Gomis-Ruth, F.X.
External linksNat Commun / PubMed:35915115
MethodsX-ray diffraction
Resolution1.8 - 2.35 Å
Structure data

PDB-7zu8:
Crystal Structure of the zymogen form of the glutamic-class prolyl-endopeptidase neprosin at 2.05 A resolution in presence of the crystallophore Lu-Xo4.
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-7zva:
Crystal Structure of the native zymogen form of the glutamic-class prolyl-endopeptidase neprosin at 1.80 A resolution.
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-7zvb:
Crystal Structure of the mature form of the glutamic-class prolyl-endopeptidase neprosin at 2.35 A resolution.
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-7zvc:
Second crystal form of the mature glutamic-class prolyl-endopeptidase neprosin at 1.85 A resolution.
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-K93:
12-oxidanyl-9,11$l^{3}-dioxa-1$l^{4},19$l^{4},22,27$l^{4},28$l^{4}-pentaza-10$l^{6}-lutetaoctacyclo[17.5.2.1^{3,7}.1^{10,13}.0^{1,10}.0^{10,19}.0^{10,28}.0^{17,27}]octacosa-3,5,7(28),11,13,15,17(27)-heptaen-8-one

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-SO4:
SULFATE ION

ChemComp-NI:
NICKEL (II) ION

ChemComp-GLY:
GLYCINE

Source
  • nepenthes ventricosa x nepenthes alata (plant)
KeywordsHYDROLASE / glutamic endopeptidase / zymogen / proform / coeliac disease therapy / crystallophore / plant protease / pitcher plant / mature form

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