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-Structure paper
| タイトル | The coupling mechanism of mammalian mitochondrial complex I. |
|---|---|
| ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 29, Issue 2, Page 172-182, Year 2022 |
| 掲載日 | 2022年2月10日 |
 著者 | Jinke Gu / Tianya Liu / Runyu Guo / Laixing Zhang / Maojun Yang /  |
| PubMed 要旨 | Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in ...Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in mitochondria. In the present study, we report cryo-electron microscopy structures of CI from Sus scrofa in six treatment conditions at a resolution of 2.4-3.5 Å, in which CI structures of each condition can be classified into two biochemical classes (active or deactive), with a notably higher proportion of active CI particles. These structures illuminate how hydrophobic ubiquinone-10 (Q10) with its long isoprenoid tail is bound and reduced in a narrow Q chamber comprising four different Q10-binding sites. Structural comparisons of active CI structures from our decylubiquinone-NADH and rotenone-NADH datasets reveal that Q10 reduction at site 1 is not coupled to proton pumping in the membrane arm, which might instead be coupled to Q10 oxidation at site 2. Our data overturn the widely accepted previous proposal about the coupling mechanism of CI. |
 リンク | Nat Struct Mol Biol / PubMed:35145322 |
| 手法 | EM (単粒子) |
| 解像度 | 2.4 - 3.5 Å |
| 構造データ | EMDB-31640, PDB-7v2c: EMDB-31641, PDB-7v2d: EMDB-31643, PDB-7v2e: EMDB-31644, PDB-7v2f: EMDB-31645, PDB-7v2h: EMDB-31646, PDB-7v2k: EMDB-31647, PDB-7v2r: EMDB-31648, PDB-7v30: EMDB-31649, PDB-7v31: EMDB-31650, PDB-7v32: EMDB-31651, PDB-7v33: EMDB-31652, PDB-7v3m: EMDB-31874, PDB-7vb7: EMDB-31881, PDB-7vbl: EMDB-31883, PDB-7vbn: EMDB-31884, PDB-7vbp: EMDB-31886, PDB-7vbz: EMDB-31887, PDB-7vc0: EMDB-32154, PDB-7vwj: EMDB-32155, PDB-7vwl: EMDB-32186, PDB-7vxp: EMDB-32187, PDB-7vxs: EMDB-32188, PDB-7vxu: EMDB-32191, PDB-7vy1: EMDB-32196, PDB-7vy8: EMDB-32197, PDB-7vy9: EMDB-32198, PDB-7vya: EMDB-32202, PDB-7vye: EMDB-32203, PDB-7vyf: EMDB-32204, PDB-7vyg: EMDB-32205, PDB-7vyh: EMDB-32206, PDB-7vyi: EMDB-32210, PDB-7vyn: EMDB-32214, PDB-7vys: EMDB-32218, PDB-7vz1: EMDB-32222, PDB-7vz8: EMDB-32230, PDB-7vzv: EMDB-32231, PDB-7vzw: EMDB-32232, PDB-7w00: EMDB-32242, PDB-7w0h: EMDB-32248, PDB-7w0r: EMDB-32249, PDB-7w0y: EMDB-32253, PDB-7w1o: EMDB-32254, PDB-7w1p: EMDB-32255, PDB-7w1t: EMDB-32256, PDB-7w1u: EMDB-32257, PDB-7w1v: EMDB-32259, PDB-7w1z: EMDB-32260, PDB-7w20: EMDB-32263, PDB-7w2k: EMDB-32264, PDB-7w2l: EMDB-32265, PDB-7w2r: EMDB-32266, PDB-7w2u: EMDB-32267, PDB-7w2y: EMDB-32269, PDB-7w31: EMDB-32270, PDB-7w32: EMDB-32271, PDB-7w35: EMDB-32300, PDB-7w4c: EMDB-32301, PDB-7w4d: EMDB-32302, PDB-7w4e: EMDB-32303, PDB-7w4f: EMDB-32304, PDB-7w4g: EMDB-32305, PDB-7w4j: EMDB-32306, PDB-7w4k: EMDB-32307, PDB-7w4l: EMDB-32308, PDB-7w4m: EMDB-32309, PDB-7w4n: EMDB-32312, PDB-7w4q: |
| 化合物 |  ChemComp-SF4:  ChemComp-FMN:  ChemComp-PEE:  ChemComp-PLX:  ChemComp-8Q1:  ChemComp-NDP:  ChemComp-UQ:  ChemComp-CDL:  ChemComp-FES:  ChemComp-MG:  ChemComp-ZN:  ChemComp-ADP:  ChemComp-NAI:  ChemComp-DCQ:  ChemComp-UQ1:  ChemComp-970:  ChemComp-HOH:  ChemComp-TYR: |
| 由来 |
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 キーワード |  ELECTRON TRANSPORT (電子伝達系) / Respiratory (呼吸器) / Complex / mammalian (哺乳類) / complex I (NADH:ユビキノン還元酵素 (水素イオン輸送型)) / mitochondrial (ミトコンドリア) / ELECTRON T |
