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Structure paper

TitleCompact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation
Journal, issue, pagesNat Commun, Vol. 13, Page 3388, Year 2022
Publish dateApr 11, 2022 (structure data deposition date)
AuthorsBasu RS / Sherman MB / Gagnon MG
External linksNat Commun / Search PubMed
MethodsEM (single particle)
Resolution2.6 - 3.6 Å
Structure data

EMDB-26628: Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-A)
Method: EM (single particle) / Resolution: 2.9 Å

26629

7unq

EMDB-26629, PDB-7unq:
Compact IF2-GDP bound to the Pseudomonas aeruginosa 70S ribosome initiation complex, from focused classification and refinement (I-A)
Method: EM (single particle) / Resolution: 3.4 Å

26630

7unr

EMDB-26630, PDB-7unr:
Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-26631: Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-B)
Method: EM (single particle) / Resolution: 2.9 Å

26632

7unt

EMDB-26632, PDB-7unt:
Compact IF2-GDP bound to the Pseudomonas aeruginosa 70S ribosome initiation complex, from focused classification and refinement (I-B)
Method: EM (single particle) / Resolution: 3.6 Å

26633

7unu

EMDB-26633, PDB-7unu:
Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)
Method: EM (single particle) / Resolution: 2.9 Å

26634

7unv

EMDB-26634, PDB-7unv:
Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)
Method: EM (single particle) / Resolution: 2.7 Å

26635

7unw

EMDB-26635, PDB-7unw:
Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)
Method: EM (single particle) / Resolution: 2.6 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-FME:
N-FORMYLMETHIONINE

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GCP:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-PCP, energy-carrying molecule analogue*YM

Source
  • pseudomonas aeruginosa pao1 (bacteria)
  • escherichia coli (E. coli)
  • bacteriophage sp. (virus)
KeywordsRIBOSOME / Initiation Factor 2 / 70S ribosome / cryo-EM / translation initiation / initiator tRNA / conformational changes

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