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- EMDB-26628: Pseudomonas aeruginosa 70S ribosome initiation complex bound to c... -
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Open data
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Basic information
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Title | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-A) | |||||||||
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![]() | Initiation Factor 2 / 70S ribosome / cryo-EM / translation initiation / initiator tRNA / conformational changes / RIBOSOME | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
![]() | Basu RS / Sherman MB / Gagnon MG | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation Authors: Basu RS / Sherman MB / Gagnon MG | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 256.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.5 KB 16.5 KB | Display Display | ![]() |
Images | ![]() | 215.3 KB | ||
Masks | ![]() | 512 MB | ![]() | |
Filedesc metadata | ![]() | 4.2 KB | ||
Others | ![]() ![]() ![]() | 408.6 MB 474.5 MB 474.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 19.1 KB | Display | |
Data in CIF | ![]() | 22.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7unqC ![]() 7unrC ![]() 7untC ![]() 7unuC ![]() 7unvC ![]() 7unwC C: citing same article ( |
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EM raw data | ![]() Data size: 1.7 TB Data #1: Unaligned multiframe micrographs of the Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2 collected on Gatan K3 in Super Resolution [micrographs - multiframe]) |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 0.85 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: Pseudomonas aeruginosa 70S ribosome initiation complex bound to...
File | emd_26628_additional_1.map | ||||||||||||
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Annotation | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-A). Sharpened map. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Pseudomonas aeruginosa 70S ribosome initiation complex bound to...
File | emd_26628_half_map_1.map | ||||||||||||
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Annotation | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-A). Half-map. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Pseudomonas aeruginosa 70S ribosome initiation complex bound to...
File | emd_26628_half_map_2.map | ||||||||||||
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Annotation | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-A). Half-map. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Pseudomonas aeruginosa 70S ribosome initiation complex bound to c...
Entire | Name: Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-A) |
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Components |
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-Supramolecule #1: Pseudomonas aeruginosa 70S ribosome initiation complex bound to c...
Supramolecule | Name: Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (I-A) type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.6 |
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Grid | Model: Quantifoil R2/1 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 295 K / Instrument: LEICA EM GP |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 8056 / Average exposure time: 1.0 sec. / Average electron dose: 31.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.1 µm / Nominal defocus min: 0.2 µm / Nominal magnification: 105000 |
Sample stage | Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |