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- EMDB-26634: Pseudomonas aeruginosa 70S ribosome initiation complex bound to I... -

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Basic information

Entry
Database: EMDB / ID: EMD-26634
TitlePseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)
Map data
Sample
  • Complex: Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)
    • RNA: x 5 types
    • Protein or peptide: x 52 types
  • Ligand: x 5 types
KeywordsInitiation Factor 2 / 70S ribosome / cryo-EM / translation initiation / initiator tRNA / conformational changes / RIBOSOME
Function / homology
Function and homology information


translation initiation factor activity / translational initiation / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / 5S rRNA binding / transferase activity / small ribosomal subunit ...translation initiation factor activity / translational initiation / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / 5S rRNA binding / transferase activity / small ribosomal subunit / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / GTP binding / RNA binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Initiation factor 2 associated domain, bacterial / Bacterial translation initiation factor IF-2 associated region / Translation initiation factor IF-2, N-terminal / Translation initiation factor IF-2, N-terminal region / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / : ...Initiation factor 2 associated domain, bacterial / Bacterial translation initiation factor IF-2 associated region / Translation initiation factor IF-2, N-terminal / Translation initiation factor IF-2, N-terminal region / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / : / Elongation factor G domain 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / Putative DNA-binding domain superfamily / Ribosomal protein L25, long-form / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L10 / : / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / : / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein L20 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L27, conserved site
Similarity search - Domain/homology
Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 ...Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Translation initiation factor IF-2 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36A / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL20 / Small ribosomal subunit protein bS21
Similarity search - Component
Biological speciesPseudomonas aeruginosa PAO1 (bacteria) / Escherichia coli (E. coli) / Bacteriophage sp. (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsBasu RS / Sherman MB / Gagnon MG
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM136936 United States
Robert A. Welch FoundationH-2032-20200401 United States
CitationJournal: Nat Commun / Year: 2022
Title: Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation
Authors: Basu RS / Sherman MB / Gagnon MG
History
DepositionApr 11, 2022-
Header (metadata) releaseJun 22, 2022-
Map releaseJun 22, 2022-
UpdateFeb 14, 2024-
Current statusFeb 14, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_26634.png

Downloads & links

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Map

FileDownload / File: emd_26634.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 512 pix.
= 435.2 Å		0.85 Å/pix.
x 512 pix.
= 435.2 Å		0.85 Å/pix.
x 512 pix.
= 435.2 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.85 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.14
Minimum - Maximum-0.673669 - 1.9991108
Average (Standard dev.)-0.0017139218 (±0.06486739)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 435.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_26634_msk_1.map
Projections & Slices
AxesZYX

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Additional map: Pseudomonas aeruginosa 70S ribosome initiation complex bound to...

Fileemd_26634_additional_1.map
AnnotationPseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A). Sharpened map.
Projections & Slices
AxesZYX

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Histogram

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Half map: Pseudomonas aeruginosa 70S ribosome initiation complex bound to...

Fileemd_26634_half_map_1.map
AnnotationPseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A). Half-map.
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Pseudomonas aeruginosa 70S ribosome initiation complex bound to...

Fileemd_26634_half_map_2.map
AnnotationPseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A). Half-map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

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Sample components

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Entire : Pseudomonas aeruginosa 70S ribosome initiation complex bound to I...

EntireName: Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)
Components
  • Complex: Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)
    • RNA: 16S Ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S21
    • RNA: P-site initiator tRNA
    • RNA: mRNA
    • Protein or peptide: Translation initiation factor IF-2
    • RNA: 23S Ribosomal RNA
    • RNA: 5S Ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L10
    • Protein or peptide: 50S ribosomal protein L11
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
  • Ligand: MAGNESIUM ION
  • Ligand: N-FORMYLMETHIONINE
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
  • Ligand: ZINC ION
  • Ligand: water

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Supramolecule #1: Pseudomonas aeruginosa 70S ribosome initiation complex bound to I...

SupramoleculeName: Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#57
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)

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Macromolecule #1: 16S Ribosomal RNA

MacromoleculeName: 16S Ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Molecular weightTheoretical: 497.973188 KDa
SequenceString: GAACUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAGCGGA UGAAGGGAGC UUGCUCCUG GAUUCAGCGG CGGACGGGUG AGUAAUGCCU AGGAAUCUGC CUGGUAGUGG GGGAUAACGU CCGGAAACGG G CGCUAAUA ...String:
GAACUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAGCGGA UGAAGGGAGC UUGCUCCUG GAUUCAGCGG CGGACGGGUG AGUAAUGCCU AGGAAUCUGC CUGGUAGUGG GGGAUAACGU CCGGAAACGG G CGCUAAUA CCGCAUACGU CCUGAGGGAG AAAGUGGGGG AUCUUCGGAC CUCACGCUAU CAGAUGAGCC UAGGUCGGAU UA GCUAGUU GGUGGGGUAA AGGCCUACCA AGGCGACGAU CCGUAACUGG UCUGAGAGGA UGAUCAGUCA CACUGGAACU GAG ACACGG UCCAGACUCC UACGGGAGGC AGCAGUGGGG AAUAUUGGAC AAUGGGCGAA AGCCUGAUCC AGCCAUGCCG CGUG UGUGA AGAAGGUCUU CGGAUUGUAA AGCACUUUAA GUUGGGAGGA AGGGCAGUAA GUUAAUACCU UGCUGUUUUG ACGUU ACCA ACAGAAUAAG CACCGGCUAA CUUCGUGCCA GCAGCC(7MG)CGG UAAUACGAAG GGUGCAAGCG UUAAUCGGAA UU ACUGGGC GUAAAGCGCG CGUAGGUGGU UCAGCAAGUU GGAUGUGAAA UCCCCGGGCU CAACCUGGGA ACUGCAUCCA AAA CUACUG AGCUAGAGUA CGGUAGAGGG UGGUGGAAUU UCCUGUGUAG CGGUGAAAUG CGUAGAUAUA GGAAGGAACA CCAG UGGCG AAGGCGACCA CCUGGACUGA UACUGACACU GAGGUGCGAA AGCGUGGGGA GCAAACAGGA UUAGAUACCC UGGUA GUCC ACGCCGUAAA CGAUGUCGAC UAGCCGUUGG GAUCCUUGAG AUCUUAGUGG CGCAGCUAAC GCGAUAAGUC GACCGC CUG GGGAGUACGG CCGCAAGGUU AAAACUCAAA UGAAUUGACG GGGGCCCGCA CAAGCGGUGG AGCAUGUGGU UUAAUUC GA A(2MG)(5MC)AACGCGA AGAACCUUAC CUGGCCUUGA CAUGCUGAGA ACUUUCCAGA GAUGGAUUGG UGCCUUCGGG AACUCAGAC ACAGGUGCUG CAUGGCUGUC GUCAGCUCGU GUCGUGAGAU GUUGGGUUAA GUCCCGUAAC GAGCGCAACC C UUGUCCUU AGUUACCAGC ACCUCGGGUG GGCACUCUAA GGAGACUGCC GGUGACAAAC CGGAGGAAGG UGGGGAUGAC GU CAAGUCA UCAUG(2MG)CCCU UACGGCCAGG GCUACACACG UGCUACAAUG GUCGGUACAA AGGGUUGCCA AGCCGCGAG GUGGAGCUAA UCCCAUAAAA CCGAUCGUAG UCCGGAUCGC AGUCUGCAAC UCGACUGCGU GAAGUCGGAA UCGCUAGUAA UCGUGAAUC AGAAUGUCAC GGUGAAUACG UUCCCGGGCC UUGUACACAC CG(4OC)CCGUCAC ACCAUGGGAG UGGGUUG CU CCAGAAGUAG CUAGUCUAAC CGCAAGGGGG ACGGUUACCA CGGAGUGAUU CAUGACUGGG GUGAAGUCG(UR3) AACA AGGUA GCCGUAGG(2MG)G (MA6)(MA6)CCUGCGGC UGGAUCACCU CCUUA

GENBANK: GENBANK: CP053028.1

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Macromolecule #22: P-site initiator tRNA

MacromoleculeName: P-site initiator tRNA / type: rna / ID: 22 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.846902 KDa
SequenceString:
CGCGGGG(4SU)GG AGCAGCCUGG UAGCUCGUCG GG(5MC)UCAUAAC CCGAAGGUCG UCGG(5MU)(PSU)CAAA UCCG GCCCC CGCAACCA

GENBANK: GENBANK: CP009106.1

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Macromolecule #23: mRNA

MacromoleculeName: mRNA / type: rna / ID: 23 / Number of copies: 1
Source (natural)Organism: Bacteriophage sp. (virus)
Molecular weightTheoretical: 7.804735 KDa
SequenceString:
GGCAAGGAGG UAAAAAUGUU CUAA

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Macromolecule #25: 23S Ribosomal RNA

MacromoleculeName: 23S Ribosomal RNA / type: rna / ID: 25 / Number of copies: 1
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Molecular weightTheoretical: 937.132125 KDa
SequenceString: GGUCAAGUGA AGAAGCGCAU ACGGUGGAUG CCUUGGCAGU CAGAGGCGAU GAAAGACGUG GUAGCCUGCG AAAAGCUUCG GGGAGUCGG CAAACAGACU UUGAUCCGGA GAUCUCUGAA UGGGGGAACC CACCUAGGAU AACCUAGGUA UCUUGUACUG A AUCCAUAG ...String:
GGUCAAGUGA AGAAGCGCAU ACGGUGGAUG CCUUGGCAGU CAGAGGCGAU GAAAGACGUG GUAGCCUGCG AAAAGCUUCG GGGAGUCGG CAAACAGACU UUGAUCCGGA GAUCUCUGAA UGGGGGAACC CACCUAGGAU AACCUAGGUA UCUUGUACUG A AUCCAUAG GUGCAAGAGG CGAACCAGGG GAACUGAAAC AUCUAAGUAC CCUGAGGAAA AGAAAUCAAC CGAGAUUCCC UU AGUAGUG GCGAGCGAAC GGGGAUUAGC CCUUAAGCUU CAUUGAUUUU AGCGGAACGC UCUGGAAAGU GCGGCCAUAG UGG GUGAUA GCCCCGUACG CGAAAGGAUC UUUGAAGUGA AAUCGAGUAG GACGGAGCAC GAGAAACUUU GUCUGAACAU GGGG GGACC AUCCUCCAAG GCUAAAUACU ACUGACUGAC CGAUAGUGAA CCAGUACCGU GAGGGAAAGG CGAAAAGAAC CCCGG AGAG GGGAGUGAAA UAGAACCUGA AACCGUAUGC GUACAAGCAG UGGGAGCCUA CUUGUUAGGU GACUGCGUAC CUUUUG UAU AAUGGGUCAG CGACUUAUAU UCAGUGGCAA GCUUAACCGU AUAGGGUAGG CGUAGCGAAA GCGAGUCUUA AUAGGGC GU UUAGUCGCUG GGUAUAGACC CGAAACCGGG CGAUCUAUCC AUGAGCAGGU UGAAGGUUAG GUAACACUGA CUGGAGGA C CGAACCCACU CCC(1MG)UUGAAA AGGUAGGGGA UGACUUGUGG AUCGGAGUGA AAGGCUAAUC AAGCUCGGAG AUAGC UGGU UCUCCUCGAA AGCUAUUUAG GUAGCGCCUC AUGUAUCACU CUGGGGGGUA GAGCACUGUU UCGGCUAGGG GGUCAU CCC GACUUACCAA ACCGAUGCAA ACUCCGAAUA CCCAGAAGUG CCGAGCAUGG GAGACACACG GCGGGUGCUA ACGUCCG UC GUGAAAAGGG AAACAACCCA GACCGCCAGC UAAGGUCCCA AAGUUGUGGU UAAGUGGUAA ACGAUGUGGG AAGGCUUA G ACAGCUAGGA GGUUGGCUUA GAAGCAGCCA CCCUUUAAAG AAAGCGUAAU AGCUCACUAG UCGAGUCGGC CUGCGCGGA AGAUGUAACG GGGCUCAAAC CACACACCGA AGCUGCGGGU GUCACGUAAG UGACGCGGUA GAGGAGCGUU CUGUAAGCCU GUGAAGGUG AGUUGAGAAG CUUGCUGGAG GUAUCAGAAG UGCGAAUGCU GACAUGAGUA ACGACAAUGG GUGUGAAAAA C ACCCACGC CGAAAGACCA AGGGUUCCUG CGCAACGUUA AUCGACGCAG GGUUAGUCGG UUCCUAAGGC GAGGCUGAAA AG CGUAGUC GAUGGGAAAC AGGUUAAUAU UCCUGUACUU CUGGUUACUG CGAUGGAGGG ACGGAGAAGG CUAGGCCAGC UUG GCGUUG GUUGUCCAAG UUUAAGGUGG UAGGCUGAAA UCUUAGGUAA AUCCGGGGUU UCAAGGCCGA GAGCUGAUGA CGAG UCGUC UUUUAGAUGA CGAAGUGGUU GAUGCCAUGC UUCCAAGAAA AGCUUCUAAG CUUCAGGUAA CCAGGAACCG UACCC CAAA CCGACAC(6MZ)GG UGGUCGGGUA GAGAAUACCA AGGCGCUUGA GAGAACUCGG GUGAAGGAAC UAGGCAAAAU GG CACCGUA ACUUCGGGAG AAGGUGCGCC GGCUAGGGUG AAGGAUUUAC UCCGUAAGCU CUGGCUGGUC GAAGAUACCA GGC CGCUGC GACUGUUUAU UAAAAACACA GCACUCUGCA AACACGAAAG UGGACGUAUA GGGUGUGACG CCU(2MG)CCCGGU GCCGGAAGG UUAAUUGAUG GGGUUAGCGC AAGCGAAGCU CUUGAUCGAA GCCCCGGUAA ACGGCGGCCG UAAC(UR3)AU AA CGGUCCUAAG GUAGCGAAA(5MU) UCCUUGUCGG GUAAGUUCCG AC(5MC)UGCACGA AUGGCGUAAC GAUGGCGGCG CUGUCUCCA CCCGAGACUC AGUGAAAUUG AAAUCGCUGU G(6MZ)AGAUGCAG UGUAUCCGCG GCUAGACGGA AAGACCC CG U(7MG)AACCUUUA CUGUAGCUUU GCACUGGACU UUGAGCCUGC UUGUGUAGGA UAGGUGGGAG GCUUUGAAGC GUGG ACGCC AGUUCGCGUG GAGCCAUCCU UGAAAUACCA CCCUGGCAUG CUUGAGGUUC UAACUCUGGU CCGUAAUCCG GAUCG AGGA CAGUGUAUGG UGGGCAGUUU GACUG(OMG)GGCG GUCUCCUCCU AAAGAGUAAC GGAGGAGUAC GAAGGUGCGC UC AGACCGG UCGGAAAUCG GUCGCAGAGU AUAAAGGCAA AAGCGCGCUU GACUGCGAGA CAGACACGUC GAGCAGGUAC GAA AGUAGG UCUUAGUGAU CCGGUGGUUC UGUAUGGAAG GGCCAUCGCU CAACGGAUAA AAGGUACUCC G(2MG)GGAUAACA GGCUGAUAC CGCCCAAGAG UUCAUAUCGA CGGCGGUGUU UGGCA(OMC)CUCG (2MA)UGUCGGCUC AUCACAUCCU GGG GCUGAA GCCGGUCCCA AGGGUAUGGC (OMU)GUUCGCCAU UUAAAGUGGU ACGCGAGCUG GGUUUAGAAC GUCGUGAGAC AGUUCGGUC CCUAUCUGCC GUGGACGUUU GAGAUUUGAG AGGGGCUGCU CCUAGUACGA GAGGACCGGA GUGGACGAAC C UCUGGUGU UCCGGUUGUC ACGCCAGUGG CAUUGCCGGG UAGCUAUGUU CGGAAAAGAU AACCGCUGAA AGCAUCUAAG CG GGAAACU UGCCUCAAGA UGAGAUCUCA CUGGGAACUU GAUUCCCCUG AAGGGCCGUC GAAGACUACG ACGUUGAUAG GCU GGGUGU GUAAGCGUUG UGAGGCGUUG AGCUAACCAG UACUAAUUGC CCGUGAGGCU UGACCAU

GENBANK: GENBANK: AE004091.2

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Macromolecule #26: 5S Ribosomal RNA

MacromoleculeName: 5S Ribosomal RNA / type: rna / ID: 26 / Number of copies: 1
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Molecular weightTheoretical: 38.596898 KDa
SequenceString:
UGCUUGACGA UCAUAGAGCG UUGGAACCAC CUGAUCCCUU CCCGAACUCA GAAGUGAAAC GACGCAUCGC CGAUGGUAGU GUGGGGUCU CCCCAUGUGA GAGUAGGUCA UCGUCAAGCU C

GENBANK: GENBANK: CP053028.1

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Macromolecule #2: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 27.376475 KDa
SequenceString: MSQVNMRDML KAGVHFGHQT RYWNPKMGKF IFGARNKIHI INLEKTLPMF NEALTFVERL AAGKNKILFV GTKRSAGKIV REEAARCGM PYVDHRWLGG MLTNYKTIRQ SIKRLRDLET QSQDGTFDKL TKKEALMRSR DLEKLERSLG GIKDMGGLPD A LFVIDVDH ...String:
MSQVNMRDML KAGVHFGHQT RYWNPKMGKF IFGARNKIHI INLEKTLPMF NEALTFVERL AAGKNKILFV GTKRSAGKIV REEAARCGM PYVDHRWLGG MLTNYKTIRQ SIKRLRDLET QSQDGTFDKL TKKEALMRSR DLEKLERSLG GIKDMGGLPD A LFVIDVDH ERIAITEANK LGIPVIGVVD TNSSPEGVDY VIPGNDDAIR AVQLYLNSMA EAVIRGKQGA ATSADEFVEE AP AESAEG

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 25.887152 KDa
SequenceString: MGQKVHPNGI RLGIVKEHTS VWYADRKNYA DYLFADLKVR EYLQDKLKSA SVSRIDIHRP AQTARITIHT ARPGIVIGKK GEDVEKLRQ DLTKQMGVPV HINIEEIRKP ELDAMLVAQS VAQQLERRVM FRRAMKRAVQ NAMRIGAKGI KIQVSGRLGG A EIARTEWY ...String:
MGQKVHPNGI RLGIVKEHTS VWYADRKNYA DYLFADLKVR EYLQDKLKSA SVSRIDIHRP AQTARITIHT ARPGIVIGKK GEDVEKLRQ DLTKQMGVPV HINIEEIRKP ELDAMLVAQS VAQQLERRVM FRRAMKRAVQ NAMRIGAKGI KIQVSGRLGG A EIARTEWY REGRVPLHTL RADIDYATYE AHTTYGVIGV KVWIFKGEVI GGRQEELKPV APAPRKKAAR

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #4: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 23.31867 KDa
SequenceString: MARYIGPKCK LSRREGTDLF LKSGARALDS KCKAENVPGQ HGQRRGRLSD YGLQLREKQK VRRIYGVLER QFRGYYQEAS RRKGSTGEN LLQLLECRLD NVVYRMGFGS TRSESRQLVS HKAITVNGQT VNIPSYQVKA GDVVAVREKS KNQLRIAQAL E LCGQRGRV ...String:
MARYIGPKCK LSRREGTDLF LKSGARALDS KCKAENVPGQ HGQRRGRLSD YGLQLREKQK VRRIYGVLER QFRGYYQEAS RRKGSTGEN LLQLLECRLD NVVYRMGFGS TRSESRQLVS HKAITVNGQT VNIPSYQVKA GDVVAVREKS KNQLRIAQAL E LCGQRGRV EWVEVDLDKK AGTFKSAPAR SDLSADINEN LIVELYSK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #5: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 17.650391 KDa
SequenceString:
MANNEQKRDE GYIEKLVQVN RVAKTVKGGR IFAFTALTVV GDGKGRVGFG RGKAREVPAA IQKAMEAARR NMIQVDLNGT TLQYPTKSA HGASKVYMQP ASEGTGIIAG GAMRAVLEVA GVQNVLAKCY GSTNPVNVVY ATFKGLKNMQ APEAVAAKRG K SVEEIL

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #6: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 16.189689 KDa
SequenceString:
MRHYEIVFLV HPDQSEQVGG MVERYTKAIE EDGGKIHRLE DWGRRQLAYA INNVHKAHYV LMNVECSAKA LAELEDNFRY NDAVIRNLV MRRDEAVTEQ SEMLKAEESR NERRERRERP NDNAEGADGD DNSDSDNADE

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #7: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 17.541514 KDa
SequenceString:
MPRRRVAAKR EVLADPKYGS QILAKFMNHV MESGKKAVAE RIVYGALDKV KERGKADPLE TFEKALDAIA PLVEVKSRRV GGATYQVPV EVRPSRRNAL AMRWLVDFAR KRGEKSMALR LAGELLDAAE GKGAAVKKRE DVHRMAEANK AFSHYRF

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #8: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 14.190609 KDa
SequenceString:
MSMQDPLADM LTRIRNAQMA EKTVVSMPSS KLKAAVAKVL KDEGYIADFQ ISSEVKPQLS IELKYFEGKP VIEEVKRISR PGLRQYKSV EQLPKVRGGL GVSIVSTNKG VMTDRAARAA GVGGEVLCTV F

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #9: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 14.643844 KDa
SequenceString:
MSATQNYGTG RRKTATARVF LRPGTGKISI NNRGLDQFFG RETARMVVRQ PLELTETVEK FDIFVTVVGG GVSGQAGAIR HGITRALIE YDETLRSSLR KAGYVTRDAR EVERKKVGLR KARKRPQYSK R

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #10: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 11.786605 KDa
SequenceString:
MQNQQIRIRL KAFDHRLIDQ STQEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD ARDQYEIRTH KRVLDIVQPT DKTVDALMK LDLAAGVEVQ ISLG

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #11: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 13.654687 KDa
SequenceString:
MAKPAARPRK KVKKTVVDGI AHIHASFNNT IVTITDRQGN ALSWATSGGS GFRGSRKSTP FAAQVAAERA GQAALEYGLK NLDVNVKGP GPGRESAVRA LNACGYKIAS ITDVTPIPHN GCRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #12: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 13.830104 KDa
SequenceString:
MATINQLVRK PRKRMVDKSD VPALQNCPQR RGVCTRVYTT TPKKPNSALR KVCRVRLTNG FEVSSYIGGE GHNLQEHSVV LIRGGRVKD LPGVRYHTVR GSLDTSGVKD RKQGRSKYGA KRPK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #13: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 13.292486 KDa
SequenceString:
MARIAGVNIP DNKHTVISLT YIYGVGRTTA QSICAATGVN PAAKIKDLSD EQIDQLRNEV AKITTEGDLR REINMNIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKPIRK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #14: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 11.591508 KDa
SequenceString:
MAKESMKNRE LKRQLTVAKY AKKRAELKAI IANPNSSAEE RWNAQVALQK QPRDASASRL RNRCRLTGRP HGFYRKFGLS RNKLREAAM RGDVPGLVKA SW

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #15: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 10.135661 KDa
SequenceString:
MALSVEEKAQ IVNEYKQAEG DTGSPEVQVA LLSANINKLQ DHFKANGKDH HSRRGLIRMV NQRRKLLDYL KGKDVSRYTA LIGRLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #16: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 9.219454 KDa
SequenceString:
MVTIRLARGG SKKRPFYHLT VTNSRNARDG RFVERIGFFN PVATGGEVRL SVDQERATYW LGQGAQPSER VAQLLKDAAK ANA

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #17: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 10.10682 KDa
SequenceString:
MAEAQKTVRT LTGRVVSDKM DKTVTVLIER RVKHPIYGKY VKRSTKLHAH DESNQCRIGD LVTIRETRPL AKTKAWTLVD IVERAVEV

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #18: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 8.892394 KDa
SequenceString:
MARFFRRRKF CRFTAEGVKE IDYKDLNTLK AYVSETGKIV PSRITGTKAK YQRQLATAIK RARYLALLPY TDSHGR

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #19: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 10.382331 KDa
SequenceString:
MPRSLKKGPF IDLHLLKKVE VAVEKNDRKP IKTWSRRSMI LPHMVGLTIA VHNGRQHVPV LVNEDMVGHK LGEFAATRTY RGHAADKKA KR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #20: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 9.942673 KDa
SequenceString:
MANTPSAKKR AKQAEKRRSH NASLRSMVRT YIKNVVKAID AKDLEKAQAA FTAAVPVIDR MADKGIIHKN KAARHKSRLS GHIKALSTA AA

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #21: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 8.508979 KDa
SequenceString:
MPAVKVKENE PFDVALRRFK RSCEKAGVLA EVRSREFYEK PTAERKRKAA AAVKRHAKKV QREQRRRERL Y

UniProtKB: Small ribosomal subunit protein bS21

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Macromolecule #24: Translation initiation factor IF-2

MacromoleculeName: Translation initiation factor IF-2 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Molecular weightTheoretical: 91.049641 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MTQVTVKELA QVVDTPVERL LLQMRDAGLP HTSAEQVVTD SEKQALLTHL KGSHGDRASE PRKITLQRKT TTTLKVGGSK TVSVEVRKK KTYVKRSPDE IEAERQRELE EQRAAEEAER LKAEEAAARQ RAEEEARKAE EAARAKAAQE AAATAGAEPA V VADVAVAE ...String:
MTQVTVKELA QVVDTPVERL LLQMRDAGLP HTSAEQVVTD SEKQALLTHL KGSHGDRASE PRKITLQRKT TTTLKVGGSK TVSVEVRKK KTYVKRSPDE IEAERQRELE EQRAAEEAER LKAEEAAARQ RAEEEARKAE EAARAKAAQE AAATAGAEPA V VADVAVAE PVAKPAAVEE RKKEEPRRVP KRDEDDDRRD RKHTQHRPSV KEKEKVPAPR VAPRSTDEES DGYRRGGRGG KS KLKKRNQ HGFQNPTGPI VREVNIGETI TVAELAAQMS VKGAEVVKFM FKMGSPVTIN QVLDQETAQL VAEELGHKVK LVS ENALEE QLAESLKFEG EAVTRAPVVT VMGHVDHGKT SLLDYIRRAK VAAGEAGGIT QHIGAYHVET ERGMVTFLDT PGHA AFTAM RARGAQATDI VILVVAADDG VMPQTQEAVQ HAKAAGVPIV VAVNKIDKPE ANPDNIKNGL AALDVIPEEW GGDAP FVPV SAKLGTGVDE LLEAVLLQAE VLELKATPSA PGRGVVVESR LDKGRGPVAT VLVQDGTLRQ GDMVLVGINY GRVRAM LDE NGKPIKEAGP SIPVEILGLD GTPDAGDEMT VVADEKKARE VALFRQGKFR EVKLARAHAG KLENIFENMG QEEKKTL NI VLKADVRGSL EALQGSLSGL GNDEVQVRVV GGGVGGITES DANLALASNA VLFGFNVRAD AGARKIVEAE GLDMRYYN V IYDIIEDVKK ALTGMLGSDL RENILGIAEV RDVFRSPKFG AIAGCMVTEG MVHRNRPIRV LRDDVVIFEG ELESLRRFK DDVAEVRAGM ECGIGVKSYN DVKVGDKIEV FEKVEVARSL

UniProtKB: Translation initiation factor IF-2

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Macromolecule #27: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 29.641256 KDa
SequenceString: MAIVKCKPTS AGRRFVVKVV NQELHKGAPY APLLEKKSKS GGRNNNGRIT TRHIGGGHKQ HYRLVDFRRN KDGIPAIVER VEYDPNRTA HIALLKYADG ERRYIIAPKG VAAGDQLISG IGAPIKAGNS MPLRNIPVGS TVHGIELKPG KGAQIARSAG A SAQLVARE ...String:
MAIVKCKPTS AGRRFVVKVV NQELHKGAPY APLLEKKSKS GGRNNNGRIT TRHIGGGHKQ HYRLVDFRRN KDGIPAIVER VEYDPNRTA HIALLKYADG ERRYIIAPKG VAAGDQLISG IGAPIKAGNS MPLRNIPVGS TVHGIELKPG KGAQIARSAG A SAQLVARE GAYVTLRLRS GEMRKVLAEC RATLGEVSNS EHSLRSLGKA GATRWRGVRP TVRGVAMNPV DHPHGGGEGR TS AGRHPVS PWGLQTKGKK TRSNKRTDNM IVRRRK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #28: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 22.625695 KDa
SequenceString: MTIGVVGRKC GMTRIFTEEG VSIPVTVIEV EPNRVTQFKT EETDGYRAVQ VTAGERRASR VTKAQAGHFA KANVAAGRGV WEFRLGEEQ YAAGDQITVD LFQAGQMVDV TGESKGKGFA GTIKRWNFRG QDNTHGNSVS HRVPGSIGQC QTPGRVFKGK K MSGHLGAE ...String:
MTIGVVGRKC GMTRIFTEEG VSIPVTVIEV EPNRVTQFKT EETDGYRAVQ VTAGERRASR VTKAQAGHFA KANVAAGRGV WEFRLGEEQ YAAGDQITVD LFQAGQMVDV TGESKGKGFA GTIKRWNFRG QDNTHGNSVS HRVPGSIGQC QTPGRVFKGK K MSGHLGAE RVTVQSLEIV RVDAERNLLL VKGAVPGATG GDVIVRPAAK ARG

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #29: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 21.673939 KDa
SequenceString: MQLNVNGAQA IEVSERTFGG EFNETLVHQA VVAYMAGGRQ GSKAQKTRSE VSGGGKKPWR QKGTGRARAG TIRSPIWRGG GTTFAAKPR SHEQKLNKKM YRAALRSILA ELVRLDRLVV VADFAVDAPK TKGLVAKLDT LGLKDVLIVT DGVDENLYLA A RNLAHVDV ...String:
MQLNVNGAQA IEVSERTFGG EFNETLVHQA VVAYMAGGRQ GSKAQKTRSE VSGGGKKPWR QKGTGRARAG TIRSPIWRGG GTTFAAKPR SHEQKLNKKM YRAALRSILA ELVRLDRLVV VADFAVDAPK TKGLVAKLDT LGLKDVLIVT DGVDENLYLA A RNLAHVDV RDVQGSDPVS LIAYDKVLVT VSAVKKFEEL LG

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #30: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 20.42483 KDa
SequenceString:
MARLKEIYRK EIAPKLKEEL QLANVMEVPR VTKITLNMGL GEAVGDKKII ENAVADLEKI TGQKPVVTYA RKSIAGFKIR EGWPIGVKV TLRSDRMYEF LDRLLSISLP RVRDFRGLNA KSFDGRGNYS MGVKEQIIFP EIDYDKIDAL RGLDITLTTT A RTDDEGRA LLRAFKFPFR N

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #31: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 19.129145 KDa
SequenceString:
MSRVAKNPVK LPAGVEIKLA GQQLSIKGAK GALELKVHPS VEVIQDSGEL RFAARNGDQQ TRAMAGTTRA LVNNMVVGVS QGFERKLQL VGVGYKAQAK GQVLSLSLGF SHPVDYELPA GIVAETPSQT DILIKGIDKQ LVGQVAAEIR DFRPPEPYKG K GVRYADEV VRRKEAKKK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #32: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 15.551678 KDa
SequenceString:
MEVILLEKVA NLGNLGDKVN IKGGYARNFL LPQGKATVAT AENVAAFEAR RAELEKAAAE KKAAAEARAA QLSELVVTLG AHAGDEGKL FGSIGTRDIA EAVSAAGYPL EKAEVRLPNG ALRNTGEFDV AVHLHTDVET TLKLIIVAE

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #33: 50S ribosomal protein L10

MacromoleculeName: 50S ribosomal protein L10 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 17.657279 KDa
SequenceString:
MAIKLEDKKA IVAEVNEAAK AALSAVVADA RGVTVGAMTG LRKEAREAGV YVKVVRNTLL KRAVEGTQFD VLNDVFKGPT LIAFSNEHP GAAARIFREF AKGQDKFEIK AAAFEGQFLA ANQIDVLASL PTYDEAVSQL MSVIQGATSK LARTLAAIRD Q KEAAAA

UniProtKB: Large ribosomal subunit protein uL10

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Macromolecule #34: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 14.925123 KDa
SequenceString:
MAKKIQAYIK LQVKAGQANP SPPVGPALGQ HGVNIMEFCK AFNAKTQGQE PGLPTPVIIT VYSDRSFTFE TKSTPAAVLL KKAAGITSG SARPNSQKVG TVTRAQLEEI AKTKQADLTA ADLDAAVRTI AGSARSMGLN VEGV

UniProtKB: Large ribosomal subunit protein uL11

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Macromolecule #35: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 16.058577 KDa
SequenceString:
MKTYTAKPET VQRDWFVVDA AGQTLGRLAT EIARRLRGKH KPEYTPHVDT GDYIVVINAE QVRVTGAKTT DKMYYHHSGF PGGIKSINF EKLIAKAPER VIETAVKGML PKNPLGRDMY RKLKVYKGAS HPHTAQQPQE LKI

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #36: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 13.43591 KDa
SequenceString:
MIQTQSMLDV ADNSGARRVM CIKVLGGSHR RYAGIGDIIK VTVKEAIPRG KVKKGQVMTA VVVRTKHGVR RTDGSIIRFD GNAAVLLNN KQEPIGTRIF GPVTRELRTE KFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #37: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 15.204488 KDa
SequenceString:
MQLNDLRSAP GARREKHRPG RGIGSGLGKT GGRGHKGLTS RSGGKVAPGF EGGQQPLHRR LPKFGFVSLK AMDRAEVRTS ELAKVEGDV VSLQTLKDAN LINQHVQRVK VMLSGEVGRA VTLKGIAATK GARAAIEAAG GKFED

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #38: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 15.434229 KDa
SequenceString:
MLQPKRTKFR KQMTGHNRGL AHRGSKVSFG EYALKATSRG RLTARQIESA RRALTRHVKR GGKIWIRVFP DKPVTKKPLE VRMGKGKGG VEYWVAQIQP GKVLYEIEGV SEELAREAFA LAAAKLPLAT SFVKRTVM

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #39: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 14.5339 KDa
SequenceString:
MRHRKSGRHL SRTSAHRKAM FQNMAVSLFE HELIKTTLPK AKELRRVAEP LITLAKEDSV ANRRLAFDRT RSKAAVGKLF NDLGKRYAN RPGGYLRILK CGFRAGDNAP MAYVELVDRP VGGEVVEAAE

UniProtKB: Large ribosomal subunit protein bL17

+
Macromolecule #40: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 12.687666 KDa
SequenceString:
MSVKKETRLR RARKARLKMR ELETVRLCVY RSSQHIYAQV IAADGGKVLA SASTLDKDLR EGATGNIDAA KKVGQLVAER AKAAGVTQV AFDRSGFKYH GRVKALADAA REGGLEF

UniProtKB: Large ribosomal subunit protein uL18

+
Macromolecule #41: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 13.055227 KDa
SequenceString:
MTNKIIQQIE AEQMNKEIPA FAPGDTVIVQ VKVKEGDRQR LQAFEGVVIA KRNRGLNSAF TVRKISNGVG VERTFQTYSP IVDSLSVKR RGDVRKAKLY YLRALSGKAA RIKEKLV

UniProtKB: Large ribosomal subunit protein bL19

+
Macromolecule #42: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 13.397972 KDa
SequenceString:
MARVKRGVIA RRRHKKILKL AKGYYGARSR VFRVAKQAVI KAGQYAYRDR RQRKRQFRAL WIARINAGAR QNGLSYSRLI AGLKKAAIE IDRKVLADLA VNEKAAFTAI VEKAKASLA

UniProtKB: Large ribosomal subunit protein bL20

+
Macromolecule #43: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 11.678621 KDa
SequenceString:
MYAVIVTGGK QHKVTEGEFL KVEKLDVATG EAIDFDRVLL VANGEDVKIG LPVVEGAKVT AEVVSHGRHD KVRIIKFRRR KHHMKRQGH RQWFTEIKIT GIQA

UniProtKB: Large ribosomal subunit protein bL21

+
Macromolecule #44: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Molecular weightTheoretical: 11.935996 KDa
SequenceString:
MEVAAKLSGA RISAQKARLV ADQIRGKKVG EALNLLAFSS KKAAEIMKKV LESAVANAEH NEGADVDDLK VSTVFVNEGR SLKRIMPRA KGRADRIVKR SCHITVKVAD K

UniProtKB: Large ribosomal subunit protein uL22

+
Macromolecule #45: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 10.969714 KDa
SequenceString:
MNQERVFKVL LGPHISEKAT GLADGKSQFV FKVATDATKL EIKKAVESLF SVKVQRVTTL NVKGKTKRTA RGLGKRNDWK KAYIALQPG QDLDFATSAE

UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #46: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 11.494491 KDa
SequenceString:
MQKIRRDDEV IVIAGKDKGK RGKVLKVLAD DRLVVGGVNL IKRHTKPNPM LGQQGGIVEK EAPLHVSNVA IFNTETSKAD RVGFKVEDG KKIRVFKSTQ KPVQA

UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #47: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 21.994242 KDa
SequenceString: MVDFILNAQV RSDLGKGASR RLRRNAGLVP AVVYGGDKEP QSVTLELREI AKLLENEAAF SHVIALNVGG AKETVLIKAL QRHPAKGFV MHADFLRVVA DHKLTAHVPL HFINEEVAVG VKQAGGEISH TISEVEVSCL PKDLPEFIEV DMAKVELGQI V HLSDLKAP ...String:
MVDFILNAQV RSDLGKGASR RLRRNAGLVP AVVYGGDKEP QSVTLELREI AKLLENEAAF SHVIALNVGG AKETVLIKAL QRHPAKGFV MHADFLRVVA DHKLTAHVPL HFINEEVAVG VKQAGGEISH TISEVEVSCL PKDLPEFIEV DMAKVELGQI V HLSDLKAP KGVELVQLAH GNDLAVANIH ASRVVKEEGS EEGAAE

UniProtKB: Large ribosomal subunit protein bL25

+
Macromolecule #48: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 9.01045 KDa
SequenceString:
MAHKKAGGST RNGRDSESKR LGVKLFGGQA VKAGNILVRQ RGTKFHAGYG VGLGKDHTLF AKVDGVVKFE TKGAFGRKYV SIVAA

UniProtKB: Large ribosomal subunit protein bL27

+
Macromolecule #49: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 9.087633 KDa
SequenceString:
MSRVCQVTGK GPVTGNNISH AHNKTRRRFL PNLQHHRFWV ESEKRFVRLR VSAKGMRIID KRGIEAVLAD LRARGEKF

UniProtKB: Large ribosomal subunit protein bL28

+
Macromolecule #50: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 7.213352 KDa
SequenceString:
MKANELREKS VEQLNEQLLG LLRDQFNLRM QKATGQLGQS HLLSQVKRDI ARVKTVLNQQ AGK

UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #51: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 6.489644 KDa
SequenceString:
MATVKVTLVK SLNGRLANHK ACVKGLGLRR INHTVEVQDT PENRGMINKA YYLLRVEG

UniProtKB: Large ribosomal subunit protein uL30

+
Macromolecule #52: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 7.934208 KDa
SequenceString:
MKADIHPTYE AIEATCSCGN VIKTRSTLCK PIHLDVCSEC HPFYTGKQKV LDTGGRIDRF KQRFGVFGAT K

UniProtKB: Large ribosomal subunit protein bL31

+
Macromolecule #53: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 6.807571 KDa
SequenceString:
MAVQQNKKSR SARDMRRSHD ALESNALSVE KSTGEVHLRH HVSPDGFYRG RKVVDKGSDE

UniProtKB: Large ribosomal subunit protein bL32

+
Macromolecule #54: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 6.060142 KDa
SequenceString:
MRELIRLVSS AGTGHFYTTD KNKRTKPEKI EIKKYDPVVR QHVIYKEAKI K

UniProtKB: Large ribosomal subunit protein bL33

+
Macromolecule #55: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 5.226309 KDa
SequenceString:
MKRTFQPSTL KRARVHGFRA RMATKNGRQV LSRRRAKGRK RLTV

UniProtKB: Large ribosomal subunit protein bL34

+
Macromolecule #56: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 7.387996 KDa
SequenceString:
MPKMKTKSGA AKRFKKTAGG LKHKHAFKSH ILTKMTTKRK RQLRGTSMLN KSDVARVERS LRLR

UniProtKB: Large ribosomal subunit protein bL35

+
Macromolecule #57: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 4.447445 KDa
SequenceString:
MKVRASVKKL CRNCKIIRRD GIVRVICSAE PRHKQRQG

UniProtKB: Large ribosomal subunit protein bL36A

+
Macromolecule #58: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 595 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #59: N-FORMYLMETHIONINE

MacromoleculeName: N-FORMYLMETHIONINE / type: ligand / ID: 59 / Number of copies: 1 / Formula: FME
Molecular weightTheoretical: 177.221 Da
Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE

+
Macromolecule #60: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 60 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

+
Macromolecule #61: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 61 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #62: water

MacromoleculeName: water / type: ligand / ID: 62 / Number of copies: 295 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R2/1 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 295 K / Instrument: LEICA EM GP

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 8056 / Average exposure time: 1.0 sec. / Average electron dose: 31.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.1 µm / Nominal defocus min: 0.2 µm / Nominal magnification: 105000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 878576
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.1.0) / Number images used: 73337
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.1.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.1.0)
Final 3D classificationSoftware - Name: cryoSPARC (ver. 3.1.0)

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Atomic model buiding 1

Initial model
PDB IDChain

6spg

source_name: PDB, initial_model_type: experimental model

3jcj

source_name: PDB, initial_model_type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-7unv:
Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)

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