Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure, dynamics and assembly of the ankyrin complex on human red blood cell membrane.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 29, Issue 7, Page 698-705, Year 2022
Publish date	Jun 2, 2022
Authors	Xian Xia / Shiheng Liu / Z Hong Zhou /
PubMed Abstract	The cytoskeleton of a red blood cell (RBC) is anchored to the cell membrane by the ankyrin complex. This complex is assembled during RBC genesis and comprises primarily band 3, protein 4.2 and ...The cytoskeleton of a red blood cell (RBC) is anchored to the cell membrane by the ankyrin complex. This complex is assembled during RBC genesis and comprises primarily band 3, protein 4.2 and ankyrin, whose mutations contribute to numerous human inherited diseases. High-resolution structures of the ankyrin complex have been long sought-after to understand its assembly and disease-causing mutations. Here, we analyzed native complexes on the human RBC membrane by stepwise fractionation. Cryo-electron microscopy structures of nine band-3-associated complexes reveal that protein 4.2 stabilizes the cytoplasmic domain of band 3 dimer. In turn, the superhelix-shaped ankyrin binds to this protein 4.2 via ankyrin repeats (ARs) 6-13 and to another band 3 dimer via ARs 17-20, bridging two band 3 dimers in the ankyrin complex. Integration of these structures with both prior data and our biochemical data supports a model of ankyrin complex assembly during erythropoiesis and identifies interactions essential for the mechanical stability of RBC.
External links	Nat Struct Mol Biol / PubMed:35655099 / PubMed Central
Methods	EM (single particle)
Resolution	3.1 - 8.5 Å
Structure data	26142 7tvz EMDB-26142, PDB-7tvz: Cryo-EM structure of human band 3-protein 4.2 complex in diagonal conformation Method: EM (single particle) / Resolution: 3.6 Å EMDB-26143: Membrane domain of band3-protein 4.2 complex in diagonal conformation Method: EM (single particle) / Resolution: 3.26 Å EMDB-26144: Cytoplasmic domain of band 3-protein 4.2 complex in diagonal conformation Method: EM (single particle) / Resolution: 3.1 Å EMDB-26145: Cryo-EM structure of human band 3-protein 4.2 complex in a loosely-bound conformation Method: EM (single particle) / Resolution: 4.1 Å 26146 7tw0 EMDB-26146, PDB-7tw0: Cryo-EM structure of human band 3-protein 4.2 complex in vertical conformation Method: EM (single particle) / Resolution: 4.6 Å 26147 7tw1 EMDB-26147, PDB-7tw1: Cryo-EM structure of human band 3-protein 4.2 complex (B2P2vertical) Method: EM (single particle) / Resolution: 4.6 Å 26148 7tw2 EMDB-26148, PDB-7tw2: Cryo-EM structure of human band 3 dimer from red blood cell Method: EM (single particle) / Resolution: 4.8 Å 26149 7tw3 EMDB-26149, PDB-7tw3: Cryo-EM structure of human ankyrin complex (B2P1A1) from red blood cell Method: EM (single particle) / Resolution: 4.4 Å EMDB-26150: Cytoplasmic domain of ankyrin complex (B2P1A1) Method: EM (single particle) / Resolution: 4.1 Å 26151 7tw5 EMDB-26151, PDB-7tw5: Cryo-EM structure of human ankyrin complex (B2P1A2) from red blood cell Method: EM (single particle) / Resolution: 5.7 Å EMDB-26152: Cytoplasmic domain of ankyrin complex (B2P1A2) Method: EM (single particle) / Resolution: 4.6 Å 26153 7tw6 EMDB-26153, PDB-7tw6: Cryo-EM structure of human ankyrin complex (B4P1A1) from red blood cell Method: EM (single particle) / Resolution: 5.6 Å EMDB-26154: Cryo-EM structure of human ankyrin complex (B2P1A1)2 Method: EM (single particle) / Resolution: 8.5 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / detergentYM ChemComp-CLR: CHOLESTEROL ChemComp-HOH*: WATER
Source	homo sapiens (human) human (human)
Keywords	MEMBRANE PROTEIN / Red blood cell / Ankyrin complex / band 3 / protein 4.2