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Structure paper

TitleStructure and assembly of the S-layer in C. difficile.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 970, Year 2022
Publish dateFeb 25, 2022
AuthorsPaola Lanzoni-Mangutchi / Oishik Banerji / Jason Wilson / Anna Barwinska-Sendra / Joseph A Kirk / Filipa Vaz / Shauna O'Beirne / Arnaud Baslé / Kamel El Omari / Armin Wagner / Neil F Fairweather / Gillian R Douce / Per A Bullough / Robert P Fagan / Paula S Salgado /
PubMed AbstractMany bacteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface and plays crucial roles in cell physiology. Here, we report the crystal structure of SlpA, ...Many bacteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface and plays crucial roles in cell physiology. Here, we report the crystal structure of SlpA, the main S-layer protein of the bacterial pathogen Clostridioides difficile, and use electron microscopy to study S-layer organisation and assembly. The SlpA crystal lattice mimics S-layer assembly in the cell, through tiling of triangular prisms above the cell wall, interlocked by distinct ridges facing the environment. Strikingly, the array is very compact, with pores of only ~10 Å in diameter, compared to other S-layers (30-100 Å). The surface-exposed flexible ridges are partially dispensable for overall structure and assembly, although a mutant lacking this region becomes susceptible to lysozyme, an important molecule in host defence. Thus, our work gives insights into S-layer organisation and provides a basis for development of C. difficile-specific therapeutics.
External linksNat Commun / PubMed:35217634 / PubMed Central
MethodsEM (electron crystallography) / X-ray diffraction
Resolution1.5 - 3.5 Å
Structure data

13957

7qgq

EMDB-13957, PDB-7qgq:
Extended H/L (SLPH/SLPL) complex from C. difficile (CD630 strain) fit into R20291 S-layer negative stain map
Method: EM (electron crystallography)

PDB-7acv:
SLPL/HID (LMW SLP complex with HMW SLP interacting domain - HID) from C. difficile (R7404 strain)
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-7acw:
LID/HID (LMW SLP and HMW SLP interacting domains) from C. difficile (R7404 strain)
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-7acx:
H/L (SLPH/SLPL) complex from C. difficile (R7404 strain)
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-7acy:
H/L (SLPH/SLPL) complex from C. difficile (CD630 strain)
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-7acz:
RdeltaD2 H/L (LMW SLP/HMW SLP) complex from C. difficile SlpA (R20291 strain)
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-SO4:
SULFATE ION / Sulfate

Source
  • clostridioides difficile r20291 (bacteria)
  • clostridioides difficile 630 (bacteria)
  • clostridioides difficile (bacteria)
  • clostridioides difficile (strain 630) (bacteria)
  • clostridioides difficile (strain r20291) (bacteria)
KeywordsSTRUCTURAL PROTEIN / bacterial surface layer protein / SLP LMW / SLP HMW interacting domain (HID) / Bacterial surface / S-layer

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