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Structure paper

TitleQuaternary structure independent folding of voltage-gated ion channel pore domain subunits.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 29, Page 537-548, Year 2022
Publish dateAug 13, 2021 (structure data deposition date)
AuthorsArrigoni, C. / Lolicato, M. / Shaya, D. / Rohaim, A. / Findeisen, F. / Fong, L.K. / Colleran, C.M. / Dominik, P. / Kim, S.S. / Schuermann, J.P. ...Arrigoni, C. / Lolicato, M. / Shaya, D. / Rohaim, A. / Findeisen, F. / Fong, L.K. / Colleran, C.M. / Dominik, P. / Kim, S.S. / Schuermann, J.P. / DeGrado, W.F. / Grabe, M. / Kossiakoff, A.A. / Minor Jr., D.L.
External linksNat. Struct. Mol. Biol. / PubMed:35655098
MethodsX-ray diffraction
Resolution2.85 - 4.5 Å
Structure data

PDB-7pg8:
NaV_Ae1/Sp1CTD_pore-ANT05 complex
Method: X-RAY DIFFRACTION / Resolution: 4.5 Å

PDB-7pgb:
NaV_Ae1/Sp1CTD_pore-SAT09 complex
Method: X-RAY DIFFRACTION / Resolution: 3.6 Å

PDB-7pgf:
Calcium-selective Sp1 channel pore domain only
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

PDB-7pgg:
NaVAb1p detergent (DM)
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

PDB-7pgh:
NaVAe1/Sp1CTDp (DDM)
Method: X-RAY DIFFRACTION / Resolution: 4.194 Å

PDB-7pgi:
NaVAb1p (bicelles)
Method: X-RAY DIFFRACTION / Resolution: 3.638 Å

Chemicals

ChemComp-4NB:
4-NITROBENZOIC ACID

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-OCT:
N-OCTANE

ChemComp-PE4:
2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL / precipitant*YM

ChemComp-LNK:
PENTANE

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-D12:
DODECANE

ChemComp-R16:
HEXADECANE

ChemComp-BHC:
BENZENE HEXACARBOXYLIC ACID

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-VVA:
2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}ethyl heptadecanoate

ChemComp-12P:
DODECAETHYLENE GLYCOL / precipitant*YM

ChemComp-ACT:
ACETATE ION

ChemComp-NA:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
  • alkalilimnicola ehrlichii mlhe-1 (bacteria)
  • ruegeria pomeroyi dss-3 (bacteria)
  • alkalilimnicola ehrlichii (strain atcc baa-1101 / dsm 17681 / mlhe-1) (bacteria)
  • ruegeria pomeroyi (strain atcc 700808 / dsm 15171 / dss-3) (bacteria)
  • ruegeria pomeroyi (bacteria)
  • alcanivorax borkumensis (bacteria)
  • alcanivorax borkumensis (strain atcc 700651 / dsm 11573 / ncimb 13689 / sk2) (bacteria)
KeywordsMEMBRANE PROTEIN / ion channel membrane protein transport protein antibody complex / folding

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