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Open data
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Basic information
Entry | Database: PDB / ID: 7pgg | ||||||||||||
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Title | NaVAb1p detergent (DM) | ||||||||||||
![]() | Ion transport protein | ||||||||||||
![]() | MEMBRANE PROTEIN / ion channel membrane protein transport protein antibody complex / folding | ||||||||||||
Function / homology | ![]() monoatomic cation channel activity / membrane => GO:0016020 / identical protein binding / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Lolicato, M. / Arrigoni, C. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Quaternary structure independent folding of voltage-gated ion channel pore domain subunits. Authors: Arrigoni, C. / Lolicato, M. / Shaya, D. / Rohaim, A. / Findeisen, F. / Fong, L.K. / Colleran, C.M. / Dominik, P. / Kim, S.S. / Schuermann, J.P. / DeGrado, W.F. / Grabe, M. / Kossiakoff, A.A. / Minor Jr., D.L. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 80.8 KB | Display | ![]() |
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PDB format | ![]() | 56.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 613 KB | Display | ![]() |
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Full document | ![]() | 621.4 KB | Display | |
Data in XML | ![]() | 13.5 KB | Display | |
Data in CIF | ![]() | 17 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7pg8C ![]() 7pgbC ![]() 7pgfC ![]() 7pghC ![]() 7pgiC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper: (Code: givenMatrix: (0.499178619406, 0.499744636522, 0.707867222151), (0.497959927409, 0.503107491957, -0.706341816851), (-0.709123837297, 0.705080243522, 0.00228770032254)Vector: 0. ...NCS oper: (Code: given Matrix: (0.499178619406, 0.499744636522, 0.707867222151), Vector: |
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Components
#1: Protein | Mass: 17148.291 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-VVA / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: SeMet labelled NavAb1 purified in DM was concentrated to 13 mg mL-1 and crystallized in 3% PEG300, 0.75M ammonium sulfate. Temp details: RT |
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-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: MAR CCD 130 mm / Detector: CCD / Date: Sep 19, 2012 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.85→50 Å / Num. obs: 31433 / % possible obs: 98.99 % / Redundancy: 16.2 % / CC1/2: 0.999 / Rmerge(I) obs: 0.169 / Net I/σ(I): 15.4 | |||||||||||||||
Reflection shell | Resolution: 2.852→2.953 Å / Num. unique obs: 3087 / CC1/2: 0.473 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 98.84 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.85→14.96 Å
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Refine LS restraints |
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LS refinement shell |
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