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TitleThe structure of the bacterial DNA segregation ATPase filament reveals the conformational plasticity of ParA upon DNA binding.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 5166, Year 2021
Publish dateAug 27, 2021
AuthorsAlexandra V Parker / Daniel Mann / Svetomir B Tzokov / Ling C Hwang / Julien R C Bergeron /
PubMed AbstractThe efficient segregation of replicated genetic material is an essential step for cell division. Bacterial cells use several evolutionarily-distinct genome segregation systems, the most common of ...The efficient segregation of replicated genetic material is an essential step for cell division. Bacterial cells use several evolutionarily-distinct genome segregation systems, the most common of which is the type I Par system. It consists of an adapter protein, ParB, that binds to the DNA cargo via interaction with the parS DNA sequence; and an ATPase, ParA, that binds nonspecific DNA and mediates cargo transport. However, the molecular details of how this system functions are not well understood. Here, we report the cryo-EM structure of the Vibrio cholerae ParA2 filament bound to DNA, as well as the crystal structures of this protein in various nucleotide states. These structures show that ParA forms a left-handed filament on DNA, stabilized by nucleotide binding, and that ParA undergoes profound structural rearrangements upon DNA binding and filament assembly. Collectively, our data suggest the structural basis for ParA's cooperative binding to DNA and the formation of high ParA density regions on the nucleoid.
External linksNat Commun / PubMed:34453062 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution2.6 - 4.5 Å
Structure data

12515

7npf

EMDB-12515, PDB-7npf:
Vibrio cholerae ParA2-ATPyS-DNA filament
Method: EM (helical sym.) / Resolution: 4.5 Å

PDB-7npd:
Vibiro cholerae ParA2
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-MG:
Unknown entry

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

Source
  • vibrio cholerae (bacteria)
  • neoarius leptaspis (salmon catfish)
KeywordsDNA BINDING PROTEIN / ATPase / Chromosome segregation / Bacterial cell division / Hydrolase / filament

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