Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape.
Journal, issue, pages	Nat Commun, Vol. 13, Issue 1, Page 4198, Year 2022
Publish date	Jul 20, 2022
Authors	Byron Hunter / Matthieu P M H Benoit / Ana B Asenjo / Caitlin Doubleday / Daria Trofimova / Corey Frazer / Irsa Shoukat / Hernando Sosa / John S Allingham /
PubMed Abstract	Kinesin-8s are dual-activity motor proteins that can move processively on microtubules and depolymerize microtubule plus-ends, but their mechanism of combining these distinct activities remains ...Kinesin-8s are dual-activity motor proteins that can move processively on microtubules and depolymerize microtubule plus-ends, but their mechanism of combining these distinct activities remains unclear. We addressed this by obtaining cryo-EM structures (2.6-3.9 Å) of Candida albicans Kip3 in different catalytic states on the microtubule lattice and on a curved microtubule end mimic. We also determined a crystal structure of microtubule-unbound CaKip3-ADP (2.0 Å) and analyzed the biochemical activity of CaKip3 and kinesin-1 mutants. These data reveal that the microtubule depolymerization activity of kinesin-8 originates from conformational changes of its motor core that are amplified by dynamic contacts between its extended loop-2 and tubulin. On curved microtubule ends, loop-1 inserts into preceding motor domains, forming head-to-tail arrays of kinesin-8s that complement loop-2 contacts with curved tubulin and assist depolymerization. On straight tubulin protofilaments in the microtubule lattice, loop-2-tubulin contacts inhibit conformational changes in the motor core, but in the ADP-Pi state these contacts are relaxed, allowing neck-linker docking for motility. We propose that these tubulin shape-induced alternations between pro-microtubule-depolymerization and pro-motility kinesin states, regulated by loop-2, are the key to the dual activity of kinesin-8 motors.
External links	Nat Commun / PubMed:35859148 / PubMed Central
Methods	EM (helical sym.) / EM (single particle) / X-ray diffraction
Resolution	2.01 - 3.9 Å
Structure data	26074 7tqx EMDB-26074, PDB-7tqx: CaKip3[2-482] - AMP-PNP in complex with a microtubule Method: EM (helical sym.) / Resolution: 2.8 Å 26075 7tqy EMDB-26075, PDB-7tqy: CaKip3[2-482] - ADP-AlFx in complex with a microtubule Method: EM (helical sym.) / Resolution: 2.6 Å 26076 7tqz EMDB-26076, PDB-7tqz: Apo CaKip3[2-482] in complex with a microtubule Method: EM (helical sym.) / Resolution: 2.7 Å 26077 7tr0 EMDB-26077, PDB-7tr0: CaKip3[2-436] - AMP-PNP in complex with a microtubule Method: EM (helical sym.) / Resolution: 2.7 Å 26078 7tr1 EMDB-26078, PDB-7tr1: CaKip3[2-436]-L2-mutant(HsKHC) - AMP-PNP in complex with a microtubule Method: EM (helical sym.) / Resolution: 3.1 Å 26079 7tr2 EMDB-26079, PDB-7tr2: Apo CaKip3[2-436]-L2-mutant(HsKHC) in complex with a microtubule Method: EM (helical sym.) / Resolution: 3.0 Å 26080 7tr3 EMDB-26080, PDB-7tr3: CaKip3[2-482] - AMP-PNP in complex with a dolastatin-10-stabilized tubulin ring Method: EM (single particle) / Resolution: 3.9 Å PDB-7lff: Crystal structure of the Candida albicans kinesin-8 motor domain Method: X-RAY DIFFRACTION / Resolution: 2.01 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-HOH: WATER ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-TA1: TAXOL / medication, chemotherapyYM ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogueYM ChemComp-AF3: ALUMINUM FLUORIDE ChemComp-SR6*: dolastatin-10
Source	candida albicans (yeast) sus scrofa (pig) candida albicans (strain sc5314 / atcc mya-2876) (yeast) homo sapiens (human)
Keywords	MOTOR PROTEIN / kinesin / microtubule / depolymerase / motility / cytoskeleton / Kip3 / tubulin / dolastatin-10