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TitleMalaria parasites use a soluble RhopH complex for erythrocyte invasion and an integral form for nutrient uptake.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateJan 4, 2021
AuthorsMarc A Schureck / Joseph E Darling / Alan Merk / Jinfeng Shao / Geervani Daggupati / Prakash Srinivasan / Paul Dominic B Olinares / Michael P Rout / Brian T Chait / Kurt Wollenberg / Sriram Subramaniam / Sanjay A Desai /
PubMed AbstractMalaria parasites use the RhopH complex for erythrocyte invasion and channel-mediated nutrient uptake. As the member proteins are unique to Plasmodium spp., how they interact and traffic through ...Malaria parasites use the RhopH complex for erythrocyte invasion and channel-mediated nutrient uptake. As the member proteins are unique to Plasmodium spp., how they interact and traffic through subcellular sites to serve these essential functions is unknown. We show that RhopH is synthesized as a soluble complex of CLAG3, RhopH2, and RhopH3 with 1:1:1 stoichiometry. After transfer to a new host cell, the complex crosses a vacuolar membrane surrounding the intracellular parasite and becomes integral to the erythrocyte membrane through a PTEX translocon-dependent process. We present a 2.9 Å single-particle cryo-electron microscopy structure of the trafficking complex, revealing that CLAG3 interacts with the other subunits over large surface areas. This soluble complex is tightly assembled with extensive disulfide bonding and predicted transmembrane helices shielded. We propose a large protein complex stabilized for trafficking but poised for host membrane insertion through large-scale rearrangements, paralleling smaller two-state pore-forming proteins in other organisms.
External linksElife / PubMed:33393463 / PubMed Central
MethodsEM (single particle)
Resolution2.92 Å
Structure data

22890

7kiy

EMDB-22890, PDB-7kiy:
Plasmodium falciparum RhopH complex in soluble form
Method: EM (single particle) / Resolution: 2.92 Å

Source
  • plasmodium falciparum (malaria parasite P. falciparum)
KeywordsMEMBRANE PROTEIN / Plasmodium falciparum / malaria / RhopH complex

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