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Structure paper

TitleCatalytic flexibility of rice glycosyltransferase OsUGT91C1 for the production of palatable steviol glycosides.
Journal, issue, pagesNat Commun, Vol. 12, Page 7030-7030, Year 2021
Publish dateMay 8, 2021 (structure data deposition date)
AuthorsZhang, J. / Tang, M. / Chen, Y. / Ke, D. / Zhou, J. / Xu, X. / Yang, W. / He, J. / Dong, H. / Wei, Y. ...Zhang, J. / Tang, M. / Chen, Y. / Ke, D. / Zhou, J. / Xu, X. / Yang, W. / He, J. / Dong, H. / Wei, Y. / Naismith, J.H. / Lin, Y. / Zhu, X. / Cheng, W.
External linksNat Commun / PubMed:34857750
MethodsX-ray diffraction
Resolution1.395 - 2.34 Å
Structure data

PDB-7erx:
Glycosyltransferase in complex with UDP and STB
Method: X-RAY DIFFRACTION / Resolution: 1.92 Å

PDB-7ery:
apo form of the glycosyltransferase
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-7es0:
a rice glycosyltransferase in complex with UDP and REX
Method: X-RAY DIFFRACTION / Resolution: 1.395 Å

PDB-7es1:
glycosyltransferase in complex with UDP and ST
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-7es2:
a mutant of glycosyktransferase in complex with UDP and Reb D
Method: X-RAY DIFFRACTION / Resolution: 2.34 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-JC6:
Steviolbioside

ChemComp-HOH:
WATER

ChemComp-3E6:
Rebaudioside A2

ChemComp-MPO:
3[N-MORPHOLINO]PROPANE SULFONIC ACID / pH buffer*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-JDF:
steviol-19-o-glucoside

ChemComp-JDO:
rebaudioside D

Source
  • oryza sativa subsp. japonica (Japanese rice)
KeywordsTRANSFERASE / glycosyltransferase in steviol glucosylation / glycosyltransferase apo form / steviol glucosylation / a rice glycosyltransferase / H27A mutant / rice glycosyltransferase

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