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Structure paper

TitleCryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end.
Journal, issue, pagesEMBO J, Vol. 40, Issue 8, Page e106164, Year 2021
Publish dateApr 15, 2021
AuthorsClinton K Lau / Francis J O'Reilly / Balaji Santhanam / Samuel E Lacey / Juri Rappsilber / Andrew P Carter /
PubMed AbstractDynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In order to do this, it forms a tripartite complex with dynein and a coiled- ...Dynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In order to do this, it forms a tripartite complex with dynein and a coiled-coil adaptor. Dynactin consists of an actin-related filament whose length is defined by its flexible shoulder domain. Despite previous cryo-EM structures, the molecular architecture of the shoulder and pointed end of the filament is still poorly understood due to the lack of high-resolution information in these regions. Here we combine multiple cryo-EM datasets and define precise masking strategies for particle signal subtraction and 3D classification. This overcomes domain flexibility and results in high-resolution maps into which we can build the shoulder and pointed end. The unique architecture of the shoulder securely houses the p150 subunit and positions the four identical p50 subunits in different conformations to bind dynactin's filament. The pointed end map allows us to build the first structure of p62 and reveals the molecular basis for cargo adaptor binding to different sites at the pointed end.
External linksEMBO J / PubMed:33734450 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 8.2 Å
Structure data

11313

6znl

EMDB-11313: Cryo-EM structure of the dynactin complex at 3.8 Angstrom resolution
PDB-6znl: Cryo-EM structure of the dynactin complex
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-11314:
The shoulder domain of dynactin with underlying Arp1 filament subnits
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-11315:
The pointed end complex of dynactin, with accompanying Arp1/actin filament subunits
Method: EM (single particle) / Resolution: 4.05 Å

EMDB-11316:
Map of the shoulder domain from dynactin to resolve the upper paddle and hook regions
Method: EM (single particle) / Resolution: 4.63 Å

11317

6znm

EMDB-11317, PDB-6znm:
The pointed end complex of dynactin bound to BICDR1
Method: EM (single particle) / Resolution: 4.1 Å

11318

6znn

EMDB-11318, PDB-6znn:
The pointed end complex of dynactin bound to Hook3
Method: EM (single particle) / Resolution: 4.5 Å

11319

6zno

EMDB-11319, PDB-6zno:
The pointed end complex of dynactin with the p150 projection docked
Method: EM (single particle) / Resolution: 6.8 Å

PDB-6zo4:
The pointed end complex of dynactin bound to BICD2
Method: ELECTRON MICROSCOPY / Resolution: 8.2 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-ZN:
Unknown entry

Source
  • sus scrofa (pig)
  • Pig (pig)
  • mus musculus (house mouse)
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / Dynactin / Complex / Scaffold / Cytoskeleton

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