EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Asymmetric Molecular Architecture of the Human γ-Tubulin Ring Complex.
ジャーナル・号・ページ	Cell, Vol. 180, Issue 1, Page 165-175.e16, Year 2020
掲載日	2020年1月9日
著者	Michal Wieczorek / Linas Urnavicius / Shih-Chieh Ti / Kelly R Molloy / Brian T Chait / Tarun M Kapoor /
PubMed 要旨	The γ-tubulin ring complex (γ-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the ...The γ-tubulin ring complex (γ-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the native human γ-TuRC at ∼3.8 Å resolution, revealing an asymmetric, cone-shaped structure. Pseudo-atomic models indicate that GCP4, GCP5, and GCP6 form distinct Y-shaped assemblies that structurally mimic GCP2/GCP3 subcomplexes distal to the γ-TuRC "seam." We also identify an unanticipated structural bridge that includes an actin-like protein and spans the γ-TuRC lumen. Despite its asymmetric architecture, the γ-TuRC arranges γ-tubulins into a helical geometry poised to nucleate microtubules. Diversity in the γ-TuRC subunits introduces large (>100,000 Å) surfaces in the complex that allow for interactions with different regulatory factors. The observed compositional complexity of the γ-TuRC could self-regulate its assembly into a cone-shaped structure to control microtubule formation across diverse contexts, e.g., within biological condensates or alongside existing filaments.
リンク	Cell / PubMed:31862189 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 6.6 Å
構造データ	21054 6v5v EMDB-21054, PDB-6v5v: Structure of gamma-tubulin in the native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 3.8 Å 21060 6v69 EMDB-21060, PDB-6v69: Structures of GCP4 and GCP5 in the native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-21063: Density map surrounding actin-like protein in reconstruction of the native gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 4.1 Å 21067 6v6b EMDB-21067, PDB-6v6b: Structures of GCP2 and GCP3 in the native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 3.8 Å 21068 6v6c EMDB-21068, PDB-6v6c: Structure of GCP6 in the native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-21069: Desnity map of lumenal bridge in reconstruction of the native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-21070: Density map for overlap region in reconstruction of native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 6.6 Å 21073 6v6s EMDB-21073, PDB-6v6s: Structure of the native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-21074: Overall density map of the native human gamma-tubulin ring complex 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN / Tubulin / gamma-tubulin / gamma-tubulin ring complex / gTuRC / g-TuRC / microtubule / microtubule nucleation / single particle cryo-EM structure / GCP / GCP4 / GCP5 / GCP2 / GCP3 / gamma-TuSC / gTuSC / GCP6