In the brain, AMPA-type glutamate receptors (AMPARs) form complexes with their auxiliary subunits and mediate the majority of fast excitatory neurotransmission. Signals transduced by these complexes ...In the brain, AMPA-type glutamate receptors (AMPARs) form complexes with their auxiliary subunits and mediate the majority of fast excitatory neurotransmission. Signals transduced by these complexes are critical for synaptic plasticity, learning, and memory. The two major categories of AMPAR auxiliary subunits are transmembrane AMPAR regulatory proteins (TARPs) and cornichon homologs (CNIHs); these subunits share little homology and play distinct roles in controlling ion channel gating and trafficking of AMPAR. Here, I report high-resolution cryo-electron microscopy structures of AMPAR in complex with CNIH3. Contrary to its predicted membrane topology, CNIH3 lacks an extracellular domain and instead contains four membrane-spanning helices. The protein-protein interaction interface that dictates channel modulation and the lipids surrounding the complex are revealed. These structures provide insights into the molecular mechanism for ion channel modulation and assembly of AMPAR/CNIH3 complexes.
EMDB-20330, PDB-6peq: GluA2 in complex with its auxiliary subunit CNIH3 - map LBD-TMD-C3 - with antagonist ZK200775 -without NTD Method: EM (single particle) / Resolution: 2.97 Å
EMDB-20332: GluA2 full length in complex with its auxiliary subunit CNIH3 in PS - with antagonist ZK200775 Method: EM (single particle) / Resolution: 4.4 Å
EMDB-20654, PDB-6u5s: NTD of GluA2 in complex with CNIH3 - with antagonist ZK200775 - in pseudo-symmetric global conformation Method: EM (single particle) / Resolution: 3.07 Å
EMDB-20666, PDB-6u6i: NTD of GluA2 in complex with CNIH3 - with antagonist ZK200775 - in asymmetric global conformation Method: EM (single particle) / Resolution: 3.12 Å
EMDB-20717: GluA2 in complex with complex full-length in AS, bound to antagonist ZK200775 Method: EM (single particle) / Resolution: 4.4 Å
EMDB-20727, PDB-6ucb: GluA2 in complex with its auxiliary subunit CNIH3 - with antagonist ZK200775, LBD, TMD, CNIH3, and lipids Method: EM (single particle) / Resolution: 3.28 Å
EMDB-20732: GluA2 in complex with its auxiliary subunit CNIH3 in AS map I - (LBD-TMD-C3(AS) I) - with antagonist ZK200775, without NTD Method: EM (single particle) / Resolution: 3.5 Å
EMDB-20733, PDB-6ud4: GluA2 in complex with its auxiliary subunit CNIH3 in AS map II - (LBD-TMD-C3(AS) II)- with antagonist ZK200775, without NTD Method: EM (single particle) / Resolution: 3.3 Å
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