Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Architecture of the mycobacterial type VII secretion system.
Journal, issue, pages	Nature, Vol. 576, Issue 7786, Page 321-325, Year 2019
Publish date	Oct 9, 2019
Authors	Nikolaos Famelis / Angel Rivera-Calzada / Gianluca Degliesposti / Maria Wingender / Nicole Mietrach / J Mark Skehel / Rafael Fernandez-Leiro / Bettina Böttcher / Andreas Schlosser / Oscar Llorca / Sebastian Geibel /
PubMed Abstract	Host infection by pathogenic mycobacteria, such as Mycobacterium tuberculosis, is facilitated by virulence factors that are secreted by type VII secretion systems. A molecular understanding of the ...Host infection by pathogenic mycobacteria, such as Mycobacterium tuberculosis, is facilitated by virulence factors that are secreted by type VII secretion systems. A molecular understanding of the type VII secretion mechanism has been hampered owing to a lack of three-dimensional structures of the fully assembled secretion apparatus. Here we report the cryo-electron microscopy structure of a membrane-embedded core complex of the ESX-3/type VII secretion system from Mycobacterium smegmatis. The core of the ESX-3 secretion machine consists of four protein components-EccB3, EccC3, EccD3 and EccE3, in a 1:1:2:1 stoichiometry-which form two identical protomers. The EccC3 coupling protein comprises a flexible array of four ATPase domains, which are linked to the membrane through a stalk domain. The domain of unknown function (DUF) adjacent to the stalk is identified as an ATPase domain that is essential for secretion. EccB3 is predominantly periplasmatic, but a small segment crosses the membrane and contacts the stalk domain. This suggests that conformational changes in the stalk domain-triggered by substrate binding at the distal end of EccC3 and subsequent ATP hydrolysis in the DUF-could be coupled to substrate secretion to the periplasm. Our results reveal that the architecture of type VII secretion systems differs markedly from that of other known secretion machines, and provide a structural understanding of these systems that will be useful for the design of antimicrobial strategies that target bacterial virulence.
External links	Nature / PubMed:31597161 / PubMed Central
Methods	EM (single particle)
Resolution	3.7 - 5.4 Å
Structure data	10186 6sgw EMDB-10186, PDB-6sgw: Structure of the ESX-3 core complex Method: EM (single particle) / Resolution: 3.8 Å 10187 6sgx EMDB-10187, PDB-6sgx: Structure of protomer 1 of the ESX-3 core complex Method: EM (single particle) / Resolution: 3.7 Å 10188 6sgy EMDB-10188, PDB-6sgy: Structure of EccB3 dimer from the ESX-3 core complex Method: EM (single particle) / Resolution: 4.6 Å EMDB-10189: ESX-3 core complex centred at the cytoplasmic region, conformation 1. Method: EM (single particle) / Resolution: 5.4 Å EMDB-10190: ESX-3 core complex centred at the cytoplasmic region, conformation 2. Method: EM (single particle) / Resolution: 5.3 Å 10191 6sgz EMDB-10191, PDB-6sgz: Structure of protomer 2 of the ESX-3 core complex Method: EM (single particle) / Resolution: 3.9 Å
Source	Mycolicibacterium smegmatis MC2 155 (bacteria) mycobacterium smegmatis (strain atcc 700084 / mc(2)155) (bacteria)
Keywords	MEMBRANE PROTEIN / Type VII Secretion System ESX-3 secretion system T7SS ESX-3 Mycobacterium smegmatis