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TitleThe substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 1296, Year 2020
Publish dateMar 10, 2020
AuthorsLucas Kuhlen / Steven Johnson / Andreas Zeitler / Sandra Bäurle / Justin C Deme / Joseph J E Caesar / Rebecca Debo / Joseph Fisher / Samuel Wagner / Susan M Lea /
PubMed AbstractProtein secretion through type-three secretion systems (T3SS) is critical for motility and virulence of many bacteria. Proteins are transported through an export gate containing three proteins ...Protein secretion through type-three secretion systems (T3SS) is critical for motility and virulence of many bacteria. Proteins are transported through an export gate containing three proteins (FliPQR in flagella, SctRST in virulence systems). A fourth essential T3SS protein (FlhB/SctU) functions to "switch" secretion substrate specificity once the growing hook/needle reach their determined length. Here, we present the cryo-electron microscopy structure of an export gate containing the switch protein from a Vibrio flagellar system at 3.2 Å resolution. The structure reveals that FlhB/SctU extends the helical export gate with its four predicted transmembrane helices wrapped around FliPQR/SctRST. The unusual topology of the FlhB/SctU helices creates a loop wrapped around the bottom of the closed export gate. Structure-informed mutagenesis suggests that this loop is critical in gating secretion and we propose that a series of conformational changes in the T3SS trigger opening of the gate through interactions between FlhB/SctU and FliPQR/SctRST.
External linksNat Commun / PubMed:32157081 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 4.1 Å
Structure data

10093

6s3l

EMDB-10093, PDB-6s3l:
Structure of the core of the flagellar export apparatus from Vibrio mimicus, the FliPQR-FlhB complex.
Method: EM (single particle) / Resolution: 3.2 Å

10095

6s3r

EMDB-10095, PDB-6s3r:
Structure of the FliPQR complex from the flagellar type 3 secretion system of Pseudomonas savastanoi.
Method: EM (single particle) / Resolution: 3.5 Å

10096

6s3s

EMDB-10096, PDB-6s3s:
Structure of the FliPQR complex from the flagellar type 3 secretion system of Vibrio mimicus.
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-10653:
Structure of the core of the flagellar export apparatus from Vibrio mimicus, the FliPQR-FlhB, in amphipol A8-35
Method: EM (single particle) / Resolution: 4.0 Å

Source
  • vibrio mimicus caim 602 (bacteria)
  • Pseudomonas savastanoi pv. phaseolicola 1448A (bacteria)
  • pseudomonas savastanoi pv. phaseolicola (strain 1448a / race 6) (bacteria)
  • pseudomonas savastanoi pv. phaseolicola (bacteria)
  • Vibrio mimicus (bacteria)
KeywordsPROTEIN TRANSPORT / flagella / T3SS / export apparatus / export gate

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