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Structure paper

TitleCryo-EM structure of the human MLL1 core complex bound to the nucleosome.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 5540, Year 2019
Publish dateDec 5, 2019
AuthorsSang Ho Park / Alex Ayoub / Young-Tae Lee / Jing Xu / Hanseong Kim / Wei Zheng / Biao Zhang / Liang Sha / Sojin An / Yang Zhang / Michael A Cianfrocco / Min Su / Yali Dou / Uhn-Soo Cho /
PubMed AbstractMixed lineage leukemia (MLL) family histone methyltransferases are enzymes that deposit histone H3 Lys4 (K4) mono-/di-/tri-methylation and regulate gene expression in mammals. Despite extensive ...Mixed lineage leukemia (MLL) family histone methyltransferases are enzymes that deposit histone H3 Lys4 (K4) mono-/di-/tri-methylation and regulate gene expression in mammals. Despite extensive structural and biochemical studies, the molecular mechanisms whereby the MLL complexes recognize histone H3K4 within nucleosome core particles (NCPs) remain unclear. Here we report the single-particle cryo-electron microscopy (cryo-EM) structure of the NCP-bound human MLL1 core complex. We show that the MLL1 core complex anchors to the NCP via the conserved RbBP5 and ASH2L, which interact extensively with nucleosomal DNA and the surface close to the N-terminal tail of histone H4. Concurrent interactions of RbBP5 and ASH2L with the NCP uniquely align the catalytic MLL1 domain at the nucleosome dyad, thereby facilitating symmetrical access to both H3K4 substrates within the NCP. Our study sheds light on how the MLL1 complex engages chromatin and how chromatin binding promotes MLL1 tri-methylation activity.
External linksNat Commun / PubMed:31804488 / PubMed Central
MethodsEM (single particle)
Resolution4.2 - 6.2 Å
Structure data

20512

6pwv

EMDB-20512: Cryo-EM structure of MLL1 in complex with RbBP5 and WDR5 bound to the nucleosome
PDB-6pwv: Cryo-EM structure of MLL1 core complex bound to the nucleosome
Method: EM (single particle) / Resolution: 6.2 Å

20513

6pww

EMDB-20513, PDB-6pww:
Cryo-EM structure of MLL1 in complex with RbBP5 and WDR5 bound to the nucleosome
Method: EM (single particle) / Resolution: 4.4 Å

20514

6pwx

EMDB-20514, PDB-6pwx:
Cryo-EM structure of RbBP5 bound to the nucleosome
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-ZN:
Unknown entry

Source
  • xenopus laevis (African clawed frog)
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsHISTONE BINDING/DNA BINDING/DNA / Mixed-Lineage Leukemia / MLL1 / nucleosome / histone H3 Lys4 methyltransferase / RbBP5 / WDR5 / HISTONE BINDING-DNA BINDING-DNA complex / ASH2L / DPY30

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