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TitleA Water-Bridged H-Bonding Network Contributes to the Catalysis of the SAM-Dependent C-Methyltransferase HcgC.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 56, Page 10806-10809, Year 2017
Publish dateMay 29, 2017 (structure data deposition date)
AuthorsBai, L. / Wagner, T. / Xu, T. / Hu, X. / Ermler, U. / Shima, S.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:28682478
MethodsX-ray diffraction
Resolution1.7 - 2.1 Å
Structure data

PDB-5o4h:
HcgC from Methanococcus maripaludis cocrystallized with SAM and pyridinol
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-5o4j:
HcgC from Methanococcus maripaludis cocrystallized with SAH and pyridinol
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5o4m:
Fresh crystals of HcgC from Methanococcus maripaludis cocrystallized with SAH and pyridinol
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5o4n:
Apo HcgC from Methanococcus maripaludis soaked with SAH and pyridinol
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-SO4:
SULFATE ION

ChemComp-ACT:
ACETATE ION

ChemComp-PE4:
2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL / precipitant*YM

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-PJL:
(3~{E})-3-[(~{E})-3-oxidanylprop-2-enoyl]iminopropanoic acid

ChemComp-LI:
Unknown entry

ChemComp-9KH:
6-carboxy methyl-4-hydroxy-2-pyridinol

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

Source
  • methanococcus maripaludis s2 (archaea)
KeywordsTRANSFERASE / methyltransferases / biosynthesis / protein structures / enzyme catalysis / mutagenesis / [Fe]-hydrogenase / pyridinol / Hmd

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