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TitleSegmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.
Journal, issue, pagesAngew Chem Int Ed Engl, Vol. 56, Issue 32, Page 9322-9325, Year 2017
Publish dateAug 1, 2017
AuthorsMiriam Sonntag / Pravin Kumar Ankush Jagtap / Bernd Simon / Marie-Sousai Appavou / Arie Geerlof / Ralf Stehle / Frank Gabel / Janosch Hennig / Michael Sattler /
PubMed AbstractMulti-domain proteins play critical roles in fine-tuning essential processes in cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers ...Multi-domain proteins play critical roles in fine-tuning essential processes in cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers undergo dynamic rearrangements upon binding to protein, DNA or RNA ligands. RNA binding proteins (RBPs) represent an important class of multi-domain proteins, which regulate gene expression by recognizing linear or structured RNA sequence motifs. Here, we employ segmental perdeuteration of the three RNA recognition motif (RRM) domains in the RBP TIA-1 using Sortase A mediated protein ligation. We show that domain-selective perdeuteration combined with contrast-matched small-angle neutron scattering (SANS), SAXS and computational modeling provides valuable information to precisely define relative domain arrangements. The approach is generally applicable to study conformational arrangements of individual domains in multi-domain proteins and changes induced by ligand binding.
External linksAngew Chem Int Ed Engl / PubMed:28636238
MethodsSAS (X-ray in house) / NMR (solution) / X-ray diffraction
Resolution2.97 Å
Structure data

SASDCC3: Nucleolysin TIA-1 isoform p40 in complex with U15 RNA
Method: SAXS/SANS

SASDCD3: Nucleolysin TIA-1 isoform p40 (LPQTG containing construct for sortase mediated protein ligation)
Method: SAXS/SANS

SASDCE3: Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)
Method: SAXS/SANS

SASDCF3: Nucleolysin TIA-1 isoform p40 (TIA-1 wild type)
Method: SAXS/SANS

SASDCG3: Nucleolysin TIA-1 isoform p40 (LPATG containing construct for sortase mediated protein ligation)
Method: SAXS/SANS

SASDCH3: Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPATG containing construct for sortase mediated protein ligation)
Method: SAXS/SANS

PDB-5o2v:
NMR structure of TIA-1 RRM1 domain
Method: SOLUTION NMR

PDB-5o3j:
Crystal structure of TIA-1 RRM2 in complex with RNA
Method: X-RAY DIFFRACTION / Resolution: 2.97 Å

Source
  • homo sapiens (human)
KeywordsRNA BINDING PROTEIN / RRM / TIA-1

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