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- SASDCD3: Nucleolysin TIA-1 isoform p40 (LPQTG containing construct for sor... -

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Basic information

Entry
Database: SASBDB / ID: SASDCD3
SampleNucleolysin TIA-1 isoform p40 (LPQTG containing construct for sortase mediated protein ligation)
  • Nucleolysin TIA-1 isoform p40 (protein), TIA-1 C-LPQTG, Homo sapiens
Function / homologyIsoform Short of Cytotoxic granule associated RNA binding protein TIA1
Function and homology information
Biological speciesHomo sapiens (human)
CitationJournal: Angew Chem Int Ed Engl / Year: 2017
Title: Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins.
Authors: Miriam Sonntag / Pravin Kumar Ankush Jagtap / Bernd Simon / Marie-Sousai Appavou / Arie Geerlof / Ralf Stehle / Frank Gabel / Janosch Hennig / Michael Sattler /
Abstract: Multi-domain proteins play critical roles in fine-tuning essential processes in cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers ...Multi-domain proteins play critical roles in fine-tuning essential processes in cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers undergo dynamic rearrangements upon binding to protein, DNA or RNA ligands. RNA binding proteins (RBPs) represent an important class of multi-domain proteins, which regulate gene expression by recognizing linear or structured RNA sequence motifs. Here, we employ segmental perdeuteration of the three RNA recognition motif (RRM) domains in the RBP TIA-1 using Sortase A mediated protein ligation. We show that domain-selective perdeuteration combined with contrast-matched small-angle neutron scattering (SANS), SAXS and computational modeling provides valuable information to precisely define relative domain arrangements. The approach is generally applicable to study conformational arrangements of individual domains in multi-domain proteins and changes induced by ligand binding.
Contact author
  • Miriam Sonntag (Technical University Munich)

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Structure visualization

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Models

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Sample

SampleName: Nucleolysin TIA-1 isoform p40 (LPQTG containing construct for sortase mediated protein ligation)
BufferName: 10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT / pH: 6
Entity #661Name: TIA-1 C-LPQTG / Type: protein / Description: Nucleolysin TIA-1 isoform p40 / Formula weight: 30.522 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P31483-2
Sequence: MEDEMPKTLY VGNLSRDVTE ALILQLFSQI GPCKNCKMIM DTAGNDPYCF VEFHEHRHAA AALAAMNGRK IMGKEVKVNW ATTPSSQKLP QTGNHFHVFV GDLSPEITTE DIKAAFAPFG RISDARVVKD MATGKSKGYG FVSFFNKWDA ENAIQQMGGQ WLGGRQIRTN ...Sequence:
MEDEMPKTLY VGNLSRDVTE ALILQLFSQI GPCKNCKMIM DTAGNDPYCF VEFHEHRHAA AALAAMNGRK IMGKEVKVNW ATTPSSQKLP QTGNHFHVFV GDLSPEITTE DIKAAFAPFG RISDARVVKD MATGKSKGYG FVSFFNKWDA ENAIQQMGGQ WLGGRQIRTN WATRKPPAPK STYESNTKQL SYDEVVNQSS PSNCTVYCGG VTSGLTEQLM RQTFSPFGQI MEIRVFPDKG YSFVRFNSHE SAAHAIVSVN GTTIEGHVVK CYWGK

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Experimental information

BeamInstrument name: Rigaku BioSAXS-1000 / City: Munich / 国: Germany / Type of source: X-ray in house
DetectorName: Pilatus 100K / Pixsize x: 172 mm
Scan
Title: Nucleolysin TIA-1 isoform p40 (LPQTG containing construct for sortase mediated protein ligation)
Measurement date: Dec 1, 2015 / Unit: 1/A /
MinMax
Q0.0166 0.6853
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 145 /
MinMax
Q0.023718 0.229466
P(R) point1 145
R0 103
Result
Type of curve: merged
ExperimentalStandardPorod
MW29.9 kDa29.9 kDa30.7 kDa
Volume--38000 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.023 0.0002 0.02288 -
Radius of gyration, Rg2.78 nm0.04 2.71 nm0.03

MinMax
D-10.3
Guinier point5 22

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