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TitleStructural and biochemical interrogation on transketolase from Pichia stipitis for new functionality
Journal, issue, pagesProtein Eng. Des. Sel., Year 2016
Publish dateJan 9, 2016 (structure data deposition date)
AuthorsHsu, L.J. / Hsu, N.S. / Wang, Y.L. / Wu, C.J. / Li, T.L.
External linksProtein Eng. Des. Sel. / PubMed:27578891
MethodsX-ray diffraction
Resolution1.031 - 1.95 Å
Structure data

PDB-5hgx:
Crystal Structure of Transketolase mutant - H261F from Pichia Stipitis
Method: X-RAY DIFFRACTION / Resolution: 1.09 Å

PDB-5hje:
Crystal Structure of Transketolase complex with sedoheptulose-7-phoaphate from Pichia Stipitis
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5hyv:
Crystal Structure of Transketolase with ThDP from Pichia Stipitis
Method: X-RAY DIFFRACTION / Resolution: 1.031 Å

PDB-5i4i:
Crystal Structure of Transketolase from Pichia Stipitis
Method: X-RAY DIFFRACTION / Resolution: 1.06 Å

PDB-5i51:
Crystal Structure of Transketolase mutant-R356L complex with fructose-6-phoaphate from Pichia Stipitis
Method: X-RAY DIFFRACTION / Resolution: 1.54 Å

PDB-5i5e:
Crystal Structure of Transketolase mutants-H66/261C complex with xylulose-5-phoaphate from Pichia Stipitis
Method: X-RAY DIFFRACTION / Resolution: 1.62 Å

PDB-5i5g:
Crystal Structure of Transketolase mutant-R525L from Pichia Stipitis
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-TPP:
THIAMINE DIPHOSPHATE

ChemComp-CA:
Unknown entry

ChemComp-I22:
D-ALTRO-HEPT-2-ULOSE 7-PHOSPHATE

ChemComp-F6R:
FRUCTOSE -6-PHOSPHATE

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-5SP:
5-O-phosphono-D-xylulose

ChemComp-POP:
PYROPHOSPHATE 2-

Source
  • scheffersomyces stipitis cbs 6054 (fungus)
KeywordsTRANSFERASE / transketolase / TKL / fructose-6-phosphate / thiamine diphosphate / xylulose-5-phosphate

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