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TitleThe modular structure of the inner-membrane ring component PrgK facilitates assembly of the type III secretion system basal body.
Journal, issue, pagesStructure, Vol. 23, Issue 1, Page 161-172, Year 2015
Publish dateJan 6, 2015
AuthorsJulien R C Bergeron / Liam J Worrall / Soumya De / Nikolaos G Sgourakis / Adrienne H Cheung / Emilie Lameignere / Mark Okon / Gregory A Wasney / David Baker / Lawrence P McIntosh / Natalie C J Strynadka /
PubMed AbstractThe type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject toxic "effector" proteins into the cells ...The type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject toxic "effector" proteins into the cells of infected organisms. The molecular details of the assembly of this large, multimembrane-spanning complex remain poorly understood. Here, we report structural, biochemical, and functional analyses of PrgK, an inner-membrane component of the prototypical Salmonella typhimurium T3SS. We have obtained the atomic structures of the two ring building globular domains and show that the C-terminal transmembrane helix is not essential for assembly and secretion. We also demonstrate that structural rearrangement of the two PrgK globular domains, driven by an interconnecting linker region, may promote oligomerization into ring structures. Finally, we used electron microscopy-guided symmetry modeling to propose a structural model for the intimately associated PrgH-PrgK ring interaction within the assembled basal body.
External linksStructure / PubMed:25533490
MethodsNMR (solution) / EM (single particle) / X-ray diffraction
Resolution2.6 - 11.7 Å
Structure data

PDB-2mky:
Structure of the PrgK first periplasmic domain
Method: SOLUTION NMR

PDB-3j6d:
Model of the PrgH-PrgK periplasmic rings
Method: ELECTRON MICROSCOPY / Resolution: 11.7 Å

PDB-4oyc:
Crystal structure of the PrgK periplasmic domain 2
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-4w4m:
Crystal structure of PrgK 19-92
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • salmonella enterica (bacteria)
  • salmonella enterica subsp. enterica serovar typhimurium str. lt2 (bacteria)
  • salmonella typhimurium (bacteria)
KeywordsCELL INVASION / Secretion systems / macromolecular assemblies / STRUCTURAL PROTEIN / Bacterial secression macromolecular assemblies / PROTEIN TRANSPORT / T3SS / macromolecular assembly / inner-membrane / Salmonella

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