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Structure paper

TitleVisualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif.
Journal, issue, pagesBiochemistry, Vol. 55, Page 788-797, Year 2016
Publish dateFeb 7, 2012 (structure data deposition date)
AuthorsBahl, C.D. / Hvorecny, K.L. / Morisseau, C. / Gerber, S.A. / Madden, D.R.
External linksBiochemistry / PubMed:26752215
MethodsX-ray diffraction
Resolution1.65 - 1.95 Å
Structure data

PDB-4dmc:
Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-4dnf:
Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the epibromohydrin hydrolysis intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-4dno:
Crystal structure of the CFTR inhibitory factor Cif with the E153Q mutation adducted with the 1,2-epoxyhexane hydrolysis intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-4ehb:
Crystal structure of the CFTR inhibitory factor Cif with the D129S mutation bound to epoxyhexane
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-4eus:
Crystal structure of the CFTR inhibitory factor Cif bound to 1,2-hexanediol
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-EBH:
(2S)-1-bromopropan-2-ol

ChemComp-7FB:
(2R)-hexane-1,2-diol

ChemComp-0PZ:
(2R)-2-butyloxirane

ChemComp-0RE:
(2S)-hexane-1,2-diol

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsHYDROLASE / Alpha Beta Hydrolase / Epoxide Hydrolase / secreted / covalently adducted with the epibromohydrin hydrolysis intermediate / Epoxide Hydrolase covalently adducted with the 1 / 2-epoxyhexane hydrolysis intermediate

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